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The map label for this gene is prfC

Identifier: 182680701

GI number: 182680701

Start: 182131

End: 183735

Strand: Direct

Name: prfC

Synonym: XfasM23_0130

Alternate gene names: 182680701

Gene position: 182131-183735 (Clockwise)

Preceding gene: 182680699

Following gene: 182680702

Centisome position: 7.18

GC content: 49.78

Gene sequence:

>1605_bases
ATGTCTGAAGTTGTTGCTGAAACAGCGCGCCGCCGTACCTTTGCCATTATTTCCCACCCTGATGCTGGGAAAACCACACT
CACCGAGAAGTTATTGTTGTTTGGTGGTGCAATTCAGATGGCTGGCTCGGTTAAAAGCCGAAAGGCTGTCCGACACGCTA
CCTCTGACTGGATGACGTTGGAAAAGGAGCGTGGTATCTCGGTTACGTCTTCAGTGATGCAGTTTCCGTATGAGGGAAAG
ATTATTAATCTGCTCGACACTCCAGGCCATGCTGACTTTGGTGAGGACACTTACCGTGTATTGACCGCAGTGGATTCAGC
TTTGATGGTGATTGATGTTGCTAAGGGTGTGGAGGAGCGGACTATTAAGTTGATGGAGGTATGTCGGTTGCGTGATACCC
CCATTATGACTTTTATCAATAAGCTTGACCGCGAAGGTAAGAACCCAATCGAGCTGCTGGATGAGGTTGAACGCGTGCTC
GGTATCCAGTGTGCGCCGGTGACTTGGCCTATTGGTATGGGGAAACGCTTGAGGGGTGTGGTTAATTTACTGACGAATGA
GGTTCATCTGTACGAGCCTGGCCGTAATTTCACTCGTCAGGACTCGATCATTTTTACGTCGCTGGAAGCTCCCGGTTTAG
CGGAACGTATTGGTGAGCAAATGCTGGCTGATTTGCATGAAGAGTTGGAGCTTATTCAGGGTGCTAGTGCATGTTTTGAT
CCAACAGAGTATCTTGGGGGCCGCCAAACTCCAGTTTTCTTTGGATCTGGTGTGAATAACTTTGGTGTGCAGCCACTGCT
GGATTTTTTCGTCGAACATGCACCTTCACCGCAACAGCGTGATACTACCAGTCGCGTCGTACTACCGACCGAAGAAAAAT
TGACCGGATTTGTATTCAAAATCCAAGCCAATATGGATCCGCAACACCGTGACCGCGTGGCGTTTATGCGGGTATGTTCC
GGACGTTTCACCGCAGGCATGAAGGCTTTTCACGTGCGTAGTAGTAAAGATCTCAAGCTTGCTAATGCATTGACCTTCAT
GGCTTCGGATCGCGAGAGCGTTGCAGAAGCTTTCCCTGGTGACGTGATTGGAATTCACAACCACGGTAGGGTTTCAATTG
GTGATACTTTCACCGAAGGGGAGGTGCTGTCGTTTACTGGCATTCCGAGTTTTGCTCCTGAACTGTTTCGCCGTGCTTGT
TTGGGTGATCCGCTGAAGCTCAAACAACTTCAAAAAGGGCTGACACAACTTTCAGAGGAAGGAGCCACACAGTTCTTTCG
CCCCCTGATGAGCAACGATCTGATTTTGGGTGCGGTAGGGATGTTGCAGTTCGATGTGGTTGCCTATCGACTTAAAAACG
AGTATGGGGTGGATGCCACTTTTGAACCAGTCAGCATTACCACCGCGCGTTGGGTGTACTGCGATAACTCAAAGACGTTG
GAAGAATTTCGCGAAAAGAACGTAACGAATTTGGCTGTGGACGCTTCAGGAGAGTTAGTTTACTTGGCGCCGACTCGAGT
CAACTTGCAGTTAGCACAGGAGCGTGCGCCAGAGATACACTTCTTCGCTACGCGCGAGCACGCCTACGCTGTAGGCGTCG
ATTGA

Upstream 100 bases:

>100_bases
TTCGGAGAGTTTTGCGTTTGGGGAGGGCGTCTGCCTGCACATAAGGCTATAATCGTAAACCTTTTTATTATTTCTTGACG
CCCAACTGGGTGCGTTTCCC

Downstream 100 bases:

>100_bases
TTGTGATTTGAGTCATAAAATAAACTCAATGATACCAATATAAGAAAATCCGAATTATTCGCGGTTATTGTAGCCTATCC
TCATGAATGCGAATGCTCAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSEVVAETARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKSRKAVRHATSDWMTLEKERGISVTSSVMQFPYEGK
IINLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIELLDEVERVL
GIQCAPVTWPIGMGKRLRGVVNLLTNEVHLYEPGRNFTRQDSIIFTSLEAPGLAERIGEQMLADLHEELELIQGASACFD
PTEYLGGRQTPVFFGSGVNNFGVQPLLDFFVEHAPSPQQRDTTSRVVLPTEEKLTGFVFKIQANMDPQHRDRVAFMRVCS
GRFTAGMKAFHVRSSKDLKLANALTFMASDRESVAEAFPGDVIGIHNHGRVSIGDTFTEGEVLSFTGIPSFAPELFRRAC
LGDPLKLKQLQKGLTQLSEEGATQFFRPLMSNDLILGAVGMLQFDVVAYRLKNEYGVDATFEPVSITTARWVYCDNSKTL
EEFREKNVTNLAVDASGELVYLAPTRVNLQLAQERAPEIHFFATREHAYAVGVD

Sequences:

>Translated_534_residues
MSEVVAETARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKSRKAVRHATSDWMTLEKERGISVTSSVMQFPYEGK
IINLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIELLDEVERVL
GIQCAPVTWPIGMGKRLRGVVNLLTNEVHLYEPGRNFTRQDSIIFTSLEAPGLAERIGEQMLADLHEELELIQGASACFD
PTEYLGGRQTPVFFGSGVNNFGVQPLLDFFVEHAPSPQQRDTTSRVVLPTEEKLTGFVFKIQANMDPQHRDRVAFMRVCS
GRFTAGMKAFHVRSSKDLKLANALTFMASDRESVAEAFPGDVIGIHNHGRVSIGDTFTEGEVLSFTGIPSFAPELFRRAC
LGDPLKLKQLQKGLTQLSEEGATQFFRPLMSNDLILGAVGMLQFDVVAYRLKNEYGVDATFEPVSITTARWVYCDNSKTL
EEFREKNVTNLAVDASGELVYLAPTRVNLQLAQERAPEIHFFATREHAYAVGVD
>Mature_533_residues
SEVVAETARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKSRKAVRHATSDWMTLEKERGISVTSSVMQFPYEGKI
INLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIELLDEVERVLG
IQCAPVTWPIGMGKRLRGVVNLLTNEVHLYEPGRNFTRQDSIIFTSLEAPGLAERIGEQMLADLHEELELIQGASACFDP
TEYLGGRQTPVFFGSGVNNFGVQPLLDFFVEHAPSPQQRDTTSRVVLPTEEKLTGFVFKIQANMDPQHRDRVAFMRVCSG
RFTAGMKAFHVRSSKDLKLANALTFMASDRESVAEAFPGDVIGIHNHGRVSIGDTFTEGEVLSFTGIPSFAPELFRRACL
GDPLKLKQLQKGLTQLSEEGATQFFRPLMSNDLILGAVGMLQFDVVAYRLKNEYGVDATFEPVSITTARWVYCDNSKTLE
EFREKNVTNLAVDASGELVYLAPTRVNLQLAQERAPEIHFFATREHAYAVGVD

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=492, Percent_Identity=26.6260162601626, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=25.6862745098039, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI25306283, Length=392, Percent_Identity=27.2959183673469, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI25306287, Length=487, Percent_Identity=26.8993839835729, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=32.1428571428571, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=31.7567567567568, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=31.0126582278481, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=61.4800759013283, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=504, Percent_Identity=26.7857142857143, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=35.4285714285714, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=527, Percent_Identity=27.134724857685, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=225, Percent_Identity=34.2222222222222, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=32.1167883211679, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=485, Percent_Identity=29.0721649484536, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=416, Percent_Identity=27.1634615384615, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324707, Length=161, Percent_Identity=31.055900621118, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=161, Percent_Identity=31.055900621118, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=27.536231884058, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=487, Percent_Identity=27.3100616016427, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI221458488, Length=507, Percent_Identity=24.0631163708087, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=32.484076433121, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_XYLF2 (B2I6P5)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828861.1
- ProteinModelPortal:   B2I6P5
- SMR:   B2I6P5
- GeneID:   6202509
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0130
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59328; Mature: 59196

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVVAETARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKSRKAVRHATSDWMTL
CCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCHHHHEE
EKERGISVTSSVMQFPYEGKIINLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEER
HHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
TIKLMEVCRLRDTPIMTFINKLDREGKNPIELLDEVERVLGIQCAPVTWPIGMGKRLRGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
VNLLTNEVHLYEPGRNFTRQDSIIFTSLEAPGLAERIGEQMLADLHEELELIQGASACFD
HHHHHCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PTEYLGGRQTPVFFGSGVNNFGVQPLLDFFVEHAPSPQQRDTTSRVVLPTEEKLTGFVFK
CHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHCEEEEE
IQANMDPQHRDRVAFMRVCSGRFTAGMKAFHVRSSKDLKLANALTFMASDRESVAEAFPG
EEECCCHHHHHHHHHHHHHCCCHHCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHCCC
DVIGIHNHGRVSIGDTFTEGEVLSFTGIPSFAPELFRRACLGDPLKLKQLQKGLTQLSEE
CEEEECCCCEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GATQFFRPLMSNDLILGAVGMLQFDVVAYRLKNEYGVDATFEPVSITTARWVYCDNSKTL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCHHH
EEFREKNVTNLAVDASGELVYLAPTRVNLQLAQERAPEIHFFATREHAYAVGVD
HHHHHCCCCEEEEECCCCEEEEECCEEEEEEHHHCCCCEEEEEECCCEEEECCC
>Mature Secondary Structure 
SEVVAETARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKSRKAVRHATSDWMTL
CHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCHHHHEE
EKERGISVTSSVMQFPYEGKIINLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEER
HHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
TIKLMEVCRLRDTPIMTFINKLDREGKNPIELLDEVERVLGIQCAPVTWPIGMGKRLRGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
VNLLTNEVHLYEPGRNFTRQDSIIFTSLEAPGLAERIGEQMLADLHEELELIQGASACFD
HHHHHCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PTEYLGGRQTPVFFGSGVNNFGVQPLLDFFVEHAPSPQQRDTTSRVVLPTEEKLTGFVFK
CHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHCEEEEE
IQANMDPQHRDRVAFMRVCSGRFTAGMKAFHVRSSKDLKLANALTFMASDRESVAEAFPG
EEECCCHHHHHHHHHHHHHCCCHHCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHCCC
DVIGIHNHGRVSIGDTFTEGEVLSFTGIPSFAPELFRRACLGDPLKLKQLQKGLTQLSEE
CEEEECCCCEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GATQFFRPLMSNDLILGAVGMLQFDVVAYRLKNEYGVDATFEPVSITTARWVYCDNSKTL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCHHH
EEFREKNVTNLAVDASGELVYLAPTRVNLQLAQERAPEIHFFATREHAYAVGVD
HHHHHCCCCEEEEECCCCEEEEECCEEEEEEHHHCCCCEEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA