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The map label for this gene is dapE

Identifier: 182680652

GI number: 182680652

Start: 109990

End: 111123

Strand: Direct

Name: dapE

Synonym: XfasM23_0081

Alternate gene names: 182680652

Gene position: 109990-111123 (Clockwise)

Preceding gene: 182680651

Following gene: 182680653

Centisome position: 4.34

GC content: 56.61

Gene sequence:

>1134_bases
ATGAGCGATGTCCTTGATCTTGCCTGCGATCTGATTTCCAGACCCTCAATGACACCGGATGATGCCGGTTGCCAGGAAAT
GATTGCAAAGCGCTTGGAGCGTGCAGGCTTCATCTGTGAACACCTGCGCTATGCTGCGGTCAGCAACCTGTGGGCTACGC
ATGGTCGAGGTGCGCCAGTGCTGGTGTTGCTTGGTCACACTGATGTGGTACCTCCCGGTCCAGTTGAAGCATGGACCTCC
GACCCGTTCATGCCAGATATGCGCAACGGCATCCTGTACGGACGCGGTGCTGCTGACATGAAAGGAAGTGTAGCCGCATT
CGTCATTGCCGCCGAGCGTTTCCTGGCTGCGTATCCCCAGCATCCAGGCACATTGGCGATCTTACTGACCTCTGATGAAG
AGGGCCAAGCAATAGATGGTGTCCGTAAGGTGGCCGAGACGTTACGCCAGCGTGGTCAGGGTATTGATTGGTGCTTGACT
GGTGAACCGTCTTCGAGCAAACGACTGGGTGACCTGCTGCGGGTCGGCCGTCGTGGCAGTTTGTCGGCAACGCTTCACGT
GAAGGGCGTCCAGGGACATGTTGCCTATCCCCATCAAGCACGCAACCCCATTCACCTCGCCTTACCTGCGTTCGCTGCAT
TGACGGCGCGGCATTGGGATGACGGTTATGAATCCTTCCCGTCAACCAGCTTGCAGATCAGCAATATCCACGCTGGAACC
GGCGCCAACAACGTGATTCCTGGTGCACTGGAGGTTGCCTTCAATCTGCGTTACAACCCGCATTGGATCGCCCCGCGCTT
GGAGAGTGAGATCGTCGCATTACTCGATCAACACGGGCTGGACTATACCTTGCACTGGCATCGTAGCGGTGAGCCGTTCT
ATACCCCGGAAGGAAAATTACGTCGCATCGCACGTGAAGTATTAGAACGTTTTTCCGGCGCCCCTCCGGAAGAAAGCACG
GGTGGCGGCACGTCCGATGCGCGTTTTATTGCGCCACTGGGTGCGCAATGTATTGAGGTTGGACCGGTCAACGCCAGCAT
TCACCAGGTTGATGAGCATGTGTGCCTGTCCGACTTGGAGGCCCTGCCAGACCTGTACCAGTTGCTAATTGAGCGGTTGC
TTGCTGAACATTGA

Upstream 100 bases:

>100_bases
GGAATTTTTTTGATTCAGTCAGGGGTTTCACAAATATCCCAAAAGAAATTTCATGTTTAAAGCGTAAGCTATCCGTTTGC
CACTCCAACTTCTTGTTCCC

Downstream 100 bases:

>100_bases
TCCTCAAATCGCTCAGGTATTCTATCGGCGAGCGTTCTTTCAGAAAAAAGAATGGTGTAAGCCAAACAGAGGCAAGCAGA
GTATCTTTGCCCAGCTTCTC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSDVLDLACDLISRPSMTPDDAGCQEMIAKRLERAGFICEHLRYAAVSNLWATHGRGAPVLVLLGHTDVVPPGPVEAWTS
DPFMPDMRNGILYGRGAADMKGSVAAFVIAAERFLAAYPQHPGTLAILLTSDEEGQAIDGVRKVAETLRQRGQGIDWCLT
GEPSSSKRLGDLLRVGRRGSLSATLHVKGVQGHVAYPHQARNPIHLALPAFAALTARHWDDGYESFPSTSLQISNIHAGT
GANNVIPGALEVAFNLRYNPHWIAPRLESEIVALLDQHGLDYTLHWHRSGEPFYTPEGKLRRIAREVLERFSGAPPEEST
GGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVCLSDLEALPDLYQLLIERLLAEH

Sequences:

>Translated_377_residues
MSDVLDLACDLISRPSMTPDDAGCQEMIAKRLERAGFICEHLRYAAVSNLWATHGRGAPVLVLLGHTDVVPPGPVEAWTS
DPFMPDMRNGILYGRGAADMKGSVAAFVIAAERFLAAYPQHPGTLAILLTSDEEGQAIDGVRKVAETLRQRGQGIDWCLT
GEPSSSKRLGDLLRVGRRGSLSATLHVKGVQGHVAYPHQARNPIHLALPAFAALTARHWDDGYESFPSTSLQISNIHAGT
GANNVIPGALEVAFNLRYNPHWIAPRLESEIVALLDQHGLDYTLHWHRSGEPFYTPEGKLRRIAREVLERFSGAPPEEST
GGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVCLSDLEALPDLYQLLIERLLAEH
>Mature_376_residues
SDVLDLACDLISRPSMTPDDAGCQEMIAKRLERAGFICEHLRYAAVSNLWATHGRGAPVLVLLGHTDVVPPGPVEAWTSD
PFMPDMRNGILYGRGAADMKGSVAAFVIAAERFLAAYPQHPGTLAILLTSDEEGQAIDGVRKVAETLRQRGQGIDWCLTG
EPSSSKRLGDLLRVGRRGSLSATLHVKGVQGHVAYPHQARNPIHLALPAFAALTARHWDDGYESFPSTSLQISNIHAGTG
ANNVIPGALEVAFNLRYNPHWIAPRLESEIVALLDQHGLDYTLHWHRSGEPFYTPEGKLRRIAREVLERFSGAPPEESTG
GGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVCLSDLEALPDLYQLLIERLLAEH

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=372, Percent_Identity=51.0752688172043, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1790395, Length=221, Percent_Identity=33.9366515837104, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789236, Length=257, Percent_Identity=22.568093385214, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_XYLF2 (B2I6B4)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828812.1
- ProteinModelPortal:   B2I6B4
- SMR:   B2I6B4
- MEROPS:   M20.010
- GeneID:   6202703
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0081
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40993; Mature: 40862

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 68-68 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVLDLACDLISRPSMTPDDAGCQEMIAKRLERAGFICEHLRYAAVSNLWATHGRGAPV
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LVLLGHTDVVPPGPVEAWTSDPFMPDMRNGILYGRGAADMKGSVAAFVIAAERFLAAYPQ
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
HPGTLAILLTSDEEGQAIDGVRKVAETLRQRGQGIDWCLTGEPSSSKRLGDLLRVGRRGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCC
LSATLHVKGVQGHVAYPHQARNPIHLALPAFAALTARHWDDGYESFPSTSLQISNIHAGT
CEEEEEEEECCCCEECCCCCCCCEEEEHHHHHHHHHHHCCCHHHHCCCCCEEEEEEECCC
GANNVIPGALEVAFNLRYNPHWIAPRLESEIVALLDQHGLDYTLHWHRSGEPFYTPEGKL
CCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RRIAREVLERFSGAPPEESTGGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVCLSDLE
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHH
ALPDLYQLLIERLLAEH
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVLDLACDLISRPSMTPDDAGCQEMIAKRLERAGFICEHLRYAAVSNLWATHGRGAPV
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LVLLGHTDVVPPGPVEAWTSDPFMPDMRNGILYGRGAADMKGSVAAFVIAAERFLAAYPQ
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
HPGTLAILLTSDEEGQAIDGVRKVAETLRQRGQGIDWCLTGEPSSSKRLGDLLRVGRRGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCC
LSATLHVKGVQGHVAYPHQARNPIHLALPAFAALTARHWDDGYESFPSTSLQISNIHAGT
CEEEEEEEECCCCEECCCCCCCCEEEEHHHHHHHHHHHCCCHHHHCCCCCEEEEEEECCC
GANNVIPGALEVAFNLRYNPHWIAPRLESEIVALLDQHGLDYTLHWHRSGEPFYTPEGKL
CCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RRIAREVLERFSGAPPEESTGGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVCLSDLE
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHH
ALPDLYQLLIERLLAEH
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA