Click here to switch to the map view.

The map label for this gene is asnB

Identifier: 182680653

GI number: 182680653

Start: 111525

End: 113216

Strand: Direct

Name: asnB

Synonym: XfasM23_0082

Alternate gene names: 182680653

Gene position: 111525-113216 (Clockwise)

Preceding gene: 182680652

Following gene: 182680655

Centisome position: 4.4

GC content: 54.96

Gene sequence:

>1692_bases
ATGTGTTCCATTTTTGGAATCTTCAACCTGCAACCCAGTGACAACCTACAGATACTGCGTCACCAAGCATTGGAGTGCTC
GCAACGGCAACGGCATCGCGGACCCGATTGGAGCGGCGTTTACGTTGATGTGGGTGCGATTCTGGTGCACGAACGTCTTG
CTATCGTCGATCCAGCCGGCGGTGCCCAGCCACTGTTCTCCGAGGATGGCACCCTGGCGTTGGCGGTCAACGGCGAAATC
TATAACCACGCTGTACTCAAAGGAACATTGCAGCAGCCCTATGCGTTCCAAACTCATTCTGATTGCGAAGTGATTAATGC
GCTTTACCGCGAAGAAACTCCAACCTCGTTTCTGAATCGCTTAAATGGAATTTTTGCATTCGCATTATGGGACAAGACCA
CCGGACGTGGCCTCATCGCACGCGATCCAATGGGCGTGGTGCCGCTGTACTGGGGGCACGATCAGGATGGTCGCTTACGC
GTCGCGTCAGAGATGAAAGCATTGGTTGAGCATTGCCCCGATGTTGCACAATTCCCACCCGGCCATTGGTACGACACCAC
AACGGGCACGCTGGTGAAGTATTACGAACGCCCCTGGAAAAACTATTCTGCAGTGGAAGGAGTGCAGGTCTCACTACAGG
AACTGCGTGAAGCCTTCGAGCGGGCCGTCCATCGTCAACTCATGACCGATGTTCCATACGGTGTACTGCTGTCTGGTGGA
TTGGATTCTTCCCTGGTGGCTGCGGTGGCCGCACGCTACGCACGCCATCGCATCGAAACCAATGATCAGAGCGAAGCATG
GTGGCCACGTCTGCACTCATTTGCGATTGGACTGAAAGACTCGCCAGATTTGAGTGCGGCGAACGTGGCTGCTGAGGCGT
TGAATACCGTCCACCATGGTTTCGAGTACACCTTCCAGGAGGGGCTGGATGTTTTACCTGAAGTGATTCGTCACATTGAA
ACTTACGATGTCACGACGATTCGCGCATCCACACCAATGTTCCTGCTGGCACGTCGCATTAAGGCGATGGGAGTGAAGAT
GGTGTTGTCAGGTGAAGGCAGCGATGAAATTTTTGGCGGTTACCTGTACTTCCACAAAGCGCCGAACGCACGCGAGTTCC
ACGAAGAATTGGTCCGTAAGCTCAACGCCCTGTATTACTACGACTGCTTGCGCGCCAACAAAGCGATGATGGCGTGGGGT
GTCGAGCCGCGCGTGCCGTTTTTGGACCGTGAATTTCTCGATGTGGCTATGCGGATGGATGCGCAGCATAAGATGGTCGA
CAAGACCAGCAACGGCCCACAACGGATGGAGAAAGGCATCCTGCGTGCAGCGTTTGACGGCTATTTGCCGCCATCAATCC
TGTGGCGACAGAAGGAGCAGTTCAGTGATGGTGTTGGCTACGGTTGGATCGACGGACTGAAAGCACACGCTGAAACGCAA
GTGTCTGACCATGCGCTGGCAACTGCCGAGACACGTTTCCAGGTGAATCCTCCCCAGACCAAAGAAGCCTATTACTACCG
CAGCATATTTGAGCGCTTCTTCCCAAGCCCGGCGGCGGCTGAGACGGTACCGGGCGGTAAATCAATCGCCTGTTCTTCAC
CGGCGGCGATTGCCTGGGATGCCAGCTTTGCCACAATGGCTGACCCATCCGGTCGTGCAGTCAGCGGGGTTCATCAACAA
GCACTGCTGTAG

Upstream 100 bases:

>100_bases
TGTTGTTTTTGTGATCTAGAGTTTCTCAGTTCCACCACCTCGCACTTGGCGATTCATCATTGCAAATCGCCAAACAAACG
CATATTCAGGAGTGCTTTCG

Downstream 100 bases:

>100_bases
TGAACGCGGTAGATTGGAGCCTGTAGTACCTCACCCAGCCTTGGGGTCACGCTGCTTCTATTGCGTCTGTACACAGGTGC
CGTAAACATCGGGTGGCGGG

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MCSIFGIFNLQPSDNLQILRHQALECSQRQRHRGPDWSGVYVDVGAILVHERLAIVDPAGGAQPLFSEDGTLALAVNGEI
YNHAVLKGTLQQPYAFQTHSDCEVINALYREETPTSFLNRLNGIFAFALWDKTTGRGLIARDPMGVVPLYWGHDQDGRLR
VASEMKALVEHCPDVAQFPPGHWYDTTTGTLVKYYERPWKNYSAVEGVQVSLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRIETNDQSEAWWPRLHSFAIGLKDSPDLSAANVAAEALNTVHHGFEYTFQEGLDVLPEVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLNALYYYDCLRANKAMMAWG
VEPRVPFLDREFLDVAMRMDAQHKMVDKTSNGPQRMEKGILRAAFDGYLPPSILWRQKEQFSDGVGYGWIDGLKAHAETQ
VSDHALATAETRFQVNPPQTKEAYYYRSIFERFFPSPAAAETVPGGKSIACSSPAAIAWDASFATMADPSGRAVSGVHQQ
ALL

Sequences:

>Translated_563_residues
MCSIFGIFNLQPSDNLQILRHQALECSQRQRHRGPDWSGVYVDVGAILVHERLAIVDPAGGAQPLFSEDGTLALAVNGEI
YNHAVLKGTLQQPYAFQTHSDCEVINALYREETPTSFLNRLNGIFAFALWDKTTGRGLIARDPMGVVPLYWGHDQDGRLR
VASEMKALVEHCPDVAQFPPGHWYDTTTGTLVKYYERPWKNYSAVEGVQVSLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRIETNDQSEAWWPRLHSFAIGLKDSPDLSAANVAAEALNTVHHGFEYTFQEGLDVLPEVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLNALYYYDCLRANKAMMAWG
VEPRVPFLDREFLDVAMRMDAQHKMVDKTSNGPQRMEKGILRAAFDGYLPPSILWRQKEQFSDGVGYGWIDGLKAHAETQ
VSDHALATAETRFQVNPPQTKEAYYYRSIFERFFPSPAAAETVPGGKSIACSSPAAIAWDASFATMADPSGRAVSGVHQQ
ALL
>Mature_563_residues
MCSIFGIFNLQPSDNLQILRHQALECSQRQRHRGPDWSGVYVDVGAILVHERLAIVDPAGGAQPLFSEDGTLALAVNGEI
YNHAVLKGTLQQPYAFQTHSDCEVINALYREETPTSFLNRLNGIFAFALWDKTTGRGLIARDPMGVVPLYWGHDQDGRLR
VASEMKALVEHCPDVAQFPPGHWYDTTTGTLVKYYERPWKNYSAVEGVQVSLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRIETNDQSEAWWPRLHSFAIGLKDSPDLSAANVAAEALNTVHHGFEYTFQEGLDVLPEVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLNALYYYDCLRANKAMMAWG
VEPRVPFLDREFLDVAMRMDAQHKMVDKTSNGPQRMEKGILRAAFDGYLPPSILWRQKEQFSDGVGYGWIDGLKAHAETQ
VSDHALATAETRFQVNPPQTKEAYYYRSIFERFFPSPAAAETVPGGKSIACSSPAAIAWDASFATMADPSGRAVSGVHQQ
ALL

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=553, Percent_Identity=37.0705244122966, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI168229252, Length=553, Percent_Identity=37.0705244122966, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI168229250, Length=553, Percent_Identity=37.0705244122966, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI296010848, Length=522, Percent_Identity=38.1226053639847, Blast_Score=293, Evalue=4e-79,
Organism=Homo sapiens, GI296010852, Length=462, Percent_Identity=38.5281385281385, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI296010850, Length=462, Percent_Identity=38.5281385281385, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1786889, Length=561, Percent_Identity=60.7843137254902, Blast_Score=688, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=571, Percent_Identity=43.9579684763573, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71993933, Length=533, Percent_Identity=45.2157598499062, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17560178, Length=528, Percent_Identity=34.0909090909091, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI25147560, Length=343, Percent_Identity=39.3586005830904, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321563, Length=590, Percent_Identity=48.4745762711864, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325403, Length=593, Percent_Identity=47.2175379426644, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45553209, Length=579, Percent_Identity=44.9050086355786, Blast_Score=455, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62931; Mature: 62931

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGIFNLQPSDNLQILRHQALECSQRQRHRGPDWSGVYVDVGAILVHERLAIVDPAG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHEEECCCC
GAQPLFSEDGTLALAVNGEIYNHAVLKGTLQQPYAFQTHSDCEVINALYREETPTSFLNR
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
LNGIFAFALWDKTTGRGLIARDPMGVVPLYWGHDQDGRLRVASEMKALVEHCPDVAQFPP
HHHEEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCHHCCCC
GHWYDTTTGTLVKYYERPWKNYSAVEGVQVSLQELREAFERAVHRQLMTDVPYGVLLSGG
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
LDSSLVAAVAARYARHRIETNDQSEAWWPRLHSFAIGLKDSPDLSAANVAAEALNTVHHG
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHC
FEYTFQEGLDVLPEVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGG
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCEEEE
YLYFHKAPNAREFHEELVRKLNALYYYDCLRANKAMMAWGVEPRVPFLDREFLDVAMRMD
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHC
AQHKMVDKTSNGPQRMEKGILRAAFDGYLPPSILWRQKEQFSDGVGYGWIDGLKAHAETQ
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VSDHALATAETRFQVNPPQTKEAYYYRSIFERFFPSPAAAETVPGGKSIACSSPAAIAWD
HHHHHHEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEC
ASFATMADPSGRAVSGVHQQALL
CCHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MCSIFGIFNLQPSDNLQILRHQALECSQRQRHRGPDWSGVYVDVGAILVHERLAIVDPAG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHEEECCCC
GAQPLFSEDGTLALAVNGEIYNHAVLKGTLQQPYAFQTHSDCEVINALYREETPTSFLNR
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
LNGIFAFALWDKTTGRGLIARDPMGVVPLYWGHDQDGRLRVASEMKALVEHCPDVAQFPP
HHHEEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCHHCCCC
GHWYDTTTGTLVKYYERPWKNYSAVEGVQVSLQELREAFERAVHRQLMTDVPYGVLLSGG
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
LDSSLVAAVAARYARHRIETNDQSEAWWPRLHSFAIGLKDSPDLSAANVAAEALNTVHHG
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHC
FEYTFQEGLDVLPEVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGG
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCEEEE
YLYFHKAPNAREFHEELVRKLNALYYYDCLRANKAMMAWGVEPRVPFLDREFLDVAMRMD
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHC
AQHKMVDKTSNGPQRMEKGILRAAFDGYLPPSILWRQKEQFSDGVGYGWIDGLKAHAETQ
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VSDHALATAETRFQVNPPQTKEAYYYRSIFERFFPSPAAAETVPGGKSIACSSPAAIAWD
HHHHHHEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEC
ASFATMADPSGRAVSGVHQQALL
CCHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]