Click here to switch to the map view.

The map label for this gene is rlmE

Identifier: 182680634

GI number: 182680634

Start: 92460

End: 93101

Strand: Reverse

Name: rlmE

Synonym: XfasM23_0063

Alternate gene names: 182680634

Gene position: 93101-92460 (Counterclockwise)

Preceding gene: 182680637

Following gene: 182680633

Centisome position: 3.67

GC content: 49.69

Gene sequence:

>642_bases
ATGTCACATAGCAAGAGCAGTCAGCGTTGGCTTAAGGAGCATTTCTCCGATCCCTTCGTGAAAAAAGCTCAAGCCGAGGG
TATGCGCTCGCGAGCCGCATACAAGTTAGAAGAAATACTTAAACGTGATCGAATCCTCAGACCGAACATGGTTGTGATCG
ATCTTGGTGCTGCACCTGGAGGATGGTCGCAGCAAATACGCAAGCAGATGGGTGATAGCGGCAGGGTCATCGCTTTGGAT
ATCGTGAAGATGGCGCCGCTGGTTGGAATAGAGTTCCTTCAAGGTGACTTCAGAGATAAAGCGGTCTTATCTCAGCTCGA
GATAATGCTCAAAGGCCAACCCGTTGACCTTGTGCTTTCAGATATGGCCCCCAATAAGAGTGGAATAGACGTAATGGACC
AACCGCGGATGATGTACTTAGCTGAGCTGGCGATGGATTTTGCGGACATCCATGTCAAACCGGGCGGTTCGTTCCTAATC
AAACTGTTCCATGGAGTCGGATCAGATGGCTACATCCGTCAATTACGACACAGGTATAAAAAAGTTGCGATCCGCAAACC
ACTTGCTTCCCGCAAGCGCTCCCCAGAGGTTTATATCCTTGGTGACGGCAAGCTCACTCAGAATGAAGTAAGCTGCTCGT
GA

Upstream 100 bases:

>100_bases
GATTTTGAGCGGAAGTGAGCGCGATGGTCATGGTTGATTTAACGGAATGCCAAATTAGATATTTAGAGTATCATGGTGGT
TCTTTTTCCTCGGTTTTCAG

Downstream 100 bases:

>100_bases
GTGGTCGCATTCGAATATTGAGAGTAAATGAGAGGCACTGGAACCAATGAGGATGAACGACTTAACCAAAAATGTGTTGC
TGTGGGTGGTCGTCGCCGTC

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSHSKSSQRWLKEHFSDPFVKKAQAEGMRSRAAYKLEEILKRDRILRPNMVVIDLGAAPGGWSQQIRKQMGDSGRVIALD
IVKMAPLVGIEFLQGDFRDKAVLSQLEIMLKGQPVDLVLSDMAPNKSGIDVMDQPRMMYLAELAMDFADIHVKPGGSFLI
KLFHGVGSDGYIRQLRHRYKKVAIRKPLASRKRSPEVYILGDGKLTQNEVSCS

Sequences:

>Translated_213_residues
MSHSKSSQRWLKEHFSDPFVKKAQAEGMRSRAAYKLEEILKRDRILRPNMVVIDLGAAPGGWSQQIRKQMGDSGRVIALD
IVKMAPLVGIEFLQGDFRDKAVLSQLEIMLKGQPVDLVLSDMAPNKSGIDVMDQPRMMYLAELAMDFADIHVKPGGSFLI
KLFHGVGSDGYIRQLRHRYKKVAIRKPLASRKRSPEVYILGDGKLTQNEVSCS
>Mature_212_residues
SHSKSSQRWLKEHFSDPFVKKAQAEGMRSRAAYKLEEILKRDRILRPNMVVIDLGAAPGGWSQQIRKQMGDSGRVIALDI
VKMAPLVGIEFLQGDFRDKAVLSQLEIMLKGQPVDLVLSDMAPNKSGIDVMDQPRMMYLAELAMDFADIHVKPGGSFLIK
LFHGVGSDGYIRQLRHRYKKVAIRKPLASRKRSPEVYILGDGKLTQNEVSCS

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=201, Percent_Identity=38.8059701492537, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI29029591, Length=189, Percent_Identity=33.8624338624339, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI29029589, Length=189, Percent_Identity=33.8624338624339, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI7110661, Length=189, Percent_Identity=33.8624338624339, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI194097365, Length=194, Percent_Identity=32.9896907216495, Blast_Score=100, Evalue=8e-22,
Organism=Escherichia coli, GI1789569, Length=202, Percent_Identity=51.980198019802, Blast_Score=224, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17553474, Length=209, Percent_Identity=32.0574162679426, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17554650, Length=187, Percent_Identity=32.620320855615, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71987550, Length=184, Percent_Identity=33.1521739130435, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=184, Percent_Identity=33.1521739130435, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17553860, Length=184, Percent_Identity=33.1521739130435, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319535, Length=197, Percent_Identity=29.9492385786802, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319796, Length=188, Percent_Identity=31.3829787234043, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321302, Length=97, Percent_Identity=35.0515463917526, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356387, Length=207, Percent_Identity=32.8502415458937, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24647580, Length=194, Percent_Identity=31.4432989690722, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18859957, Length=194, Percent_Identity=31.9587628865979, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=28.4974093264249, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_XYLF2 (B2I696)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828794.1
- ProteinModelPortal:   B2I696
- SMR:   B2I696
- GeneID:   6202396
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0063
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK445916
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23951; Mature: 23820

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 60-60 BINDING 62-62 BINDING 80-80 BINDING 96-96 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSKSSQRWLKEHFSDPFVKKAQAEGMRSRAAYKLEEILKRDRILRPNMVVIDLGAAPG
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
GWSQQIRKQMGDSGRVIALDIVKMAPLVGIEFLQGDFRDKAVLSQLEIMLKGQPVDLVLS
CHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHEEEE
DMAPNKSGIDVMDQPRMMYLAELAMDFADIHVKPGGSFLIKLFHGVGSDGYIRQLRHRYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCCHHHHHHHHHHH
KVAIRKPLASRKRSPEVYILGDGKLTQNEVSCS
HHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SHSKSSQRWLKEHFSDPFVKKAQAEGMRSRAAYKLEEILKRDRILRPNMVVIDLGAAPG
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
GWSQQIRKQMGDSGRVIALDIVKMAPLVGIEFLQGDFRDKAVLSQLEIMLKGQPVDLVLS
CHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHEEEE
DMAPNKSGIDVMDQPRMMYLAELAMDFADIHVKPGGSFLIKLFHGVGSDGYIRQLRHRYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCCHHHHHHHHHHH
KVAIRKPLASRKRSPEVYILGDGKLTQNEVSCS
HHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA