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The map label for this gene is dnaN [H]

Identifier: 182680574

GI number: 182680574

Start: 1638

End: 2738

Strand: Direct

Name: dnaN [H]

Synonym: XfasM23_0002

Alternate gene names: 182680574

Gene position: 1638-2738 (Clockwise)

Preceding gene: 182680573

Following gene: 182680575

Centisome position: 0.06

GC content: 37.42

Gene sequence:

>1101_bases
ATGCGTTTCAGATTACAGCGCGAAACTTTCCTAAAACCATTAGCTCACGTTGTAAACGTAGTAGAACGCCGTCAAACACG
TTCTATCTTGGCTAATCTCCTAATAAAAGTTAATGAAGATCAATTATCCTTAACTGGTACAGATCTTGAAGTGGAGATGA
TTTCAAAAACAAAAATTGAAGATCCGGAAAGTGGGGAGATTACTATCCCTGCACGTAAAATTTATGAAATCGTTAGGGCT
TTACCGGATGCTAGTCAACTAAGTGTATATCAATCCGATGATAAAATTACCCTCCAAGCTGGTCGAAGCAGGTTTACCTT
AGCTACATTACCAGCTAATGATTTTCCATCTATAGATAAAATCGAAGTAACGGAACGTATTATTATTGCTGAAGTTTTAT
TAAAGGAACTCATTGAGCGTACTGCTTTTGCAATGGCCCAACAAGATGTACGTTATTATTTAAATGGTTTATTGTTTGAT
TTACGAGACACTAAATTACGTTGTGTAGCTACCGATGGCCATCGTTTAGCATTGTGTGAAACTGAATTAGAACAAGCTAA
AGATCTTAAACGCCAGATTATTTTGCCGCGTAAAGGCGTAATGGAATTACAACGTTTATTAGAAGGGAGTGATCGCCAAA
TTGAGTTGGAAATTGCTCGTAACCACATTAGAATGAAATCCTTTGATGTAACGTTTACTTCAAAATTGATTGATGGTTCT
TTCCCAGATTATGAGGGAGTGATACCAATCGGTGCTGATCGTGAGGTAAAGGTTGCTCGTGAGGTTTTACGTGATGCTCT
TCAACGGGCGGCAATTTTATCTAACGAGAAATATCGTGGTGTGCGTATTGAAGTTTCACCTGGGCAGTTAAAAATTAATG
CACATAACCCAGAGCAAGAAGAAGCACAGGAAGAAATTGAGGCCCAAACTATTGTGGATGGTTTGGCGATTGGTTTTAAT
GTTAATTATTTGTTAGATGCTTTATCTTCCTTGCGTGGTGATTTTGTTAATATTCAGCTACGTGATTCAAATTCTTCTGC
TTTAATCCGTGAATCTAATAGTGAGAAGTCACTTCAAGTAGTGATGCCATTAAGATTATAA

Upstream 100 bases:

>100_bases
ATTAAACAACATCTTAGAGTACTTCTCCACAAAAAAAATTTCTACCACCACCACCATAAGTTTTAAATATTAACTAAAAA
GCATAGAGGTAAGGAACCAA

Downstream 100 bases:

>100_bases
TTTTTATTCAACTTTGATAATAAAGTACGCCTGATAATAATCGGGCGTTTTTTTGTGGAACTTAAATTTTATATTTAATT
TAATTGTTATAATAATTTGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRFRLQRETFLKPLAHVVNVVERRQTRSILANLLIKVNEDQLSLTGTDLEVEMISKTKIEDPESGEITIPARKIYEIVRA
LPDASQLSVYQSDDKITLQAGRSRFTLATLPANDFPSIDKIEVTERIIIAEVLLKELIERTAFAMAQQDVRYYLNGLLFD
LRDTKLRCVATDGHRLALCETELEQAKDLKRQIILPRKGVMELQRLLEGSDRQIELEIARNHIRMKSFDVTFTSKLIDGS
FPDYEGVIPIGADREVKVAREVLRDALQRAAILSNEKYRGVRIEVSPGQLKINAHNPEQEEAQEEIEAQTIVDGLAIGFN
VNYLLDALSSLRGDFVNIQLRDSNSSALIRESNSEKSLQVVMPLRL

Sequences:

>Translated_366_residues
MRFRLQRETFLKPLAHVVNVVERRQTRSILANLLIKVNEDQLSLTGTDLEVEMISKTKIEDPESGEITIPARKIYEIVRA
LPDASQLSVYQSDDKITLQAGRSRFTLATLPANDFPSIDKIEVTERIIIAEVLLKELIERTAFAMAQQDVRYYLNGLLFD
LRDTKLRCVATDGHRLALCETELEQAKDLKRQIILPRKGVMELQRLLEGSDRQIELEIARNHIRMKSFDVTFTSKLIDGS
FPDYEGVIPIGADREVKVAREVLRDALQRAAILSNEKYRGVRIEVSPGQLKINAHNPEQEEAQEEIEAQTIVDGLAIGFN
VNYLLDALSSLRGDFVNIQLRDSNSSALIRESNSEKSLQVVMPLRL
>Mature_366_residues
MRFRLQRETFLKPLAHVVNVVERRQTRSILANLLIKVNEDQLSLTGTDLEVEMISKTKIEDPESGEITIPARKIYEIVRA
LPDASQLSVYQSDDKITLQAGRSRFTLATLPANDFPSIDKIEVTERIIIAEVLLKELIERTAFAMAQQDVRYYLNGLLFD
LRDTKLRCVATDGHRLALCETELEQAKDLKRQIILPRKGVMELQRLLEGSDRQIELEIARNHIRMKSFDVTFTSKLIDGS
FPDYEGVIPIGADREVKVAREVLRDALQRAAILSNEKYRGVRIEVSPGQLKINAHNPEQEEAQEEIEAQTIVDGLAIGFN
VNYLLDALSSLRGDFVNIQLRDSNSSALIRESNSEKSLQVVMPLRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=46.866485013624, Blast_Score=345, Evalue=2e-96,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41549; Mature: 41549

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFRLQRETFLKPLAHVVNVVERRQTRSILANLLIKVNEDQLSLTGTDLEVEMISKTKIE
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEECCCCCC
DPESGEITIPARKIYEIVRALPDASQLSVYQSDDKITLQAGRSRFTLATLPANDFPSIDK
CCCCCEEEECHHHHHHHHHHCCCCHHEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCC
IEVTERIIIAEVLLKELIERTAFAMAQQDVRYYLNGLLFDLRDTKLRCVATDGHRLALCE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCEEEEEH
TELEQAKDLKRQIILPRKGVMELQRLLEGSDRQIELEIARNHIRMKSFDVTFTSKLIDGS
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCEEEEEECEEEEHHHHCCC
FPDYEGVIPIGADREVKVAREVLRDALQRAAILSNEKYRGVRIEVSPGQLKINAHNPEQE
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCHH
EAQEEIEAQTIVDGLAIGFNVNYLLDALSSLRGDFVNIQLRDSNSSALIRESNSEKSLQV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCEEE
VMPLRL
EEECCC
>Mature Secondary Structure
MRFRLQRETFLKPLAHVVNVVERRQTRSILANLLIKVNEDQLSLTGTDLEVEMISKTKIE
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEECCCCCC
DPESGEITIPARKIYEIVRALPDASQLSVYQSDDKITLQAGRSRFTLATLPANDFPSIDK
CCCCCEEEECHHHHHHHHHHCCCCHHEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCC
IEVTERIIIAEVLLKELIERTAFAMAQQDVRYYLNGLLFDLRDTKLRCVATDGHRLALCE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCEEEEEH
TELEQAKDLKRQIILPRKGVMELQRLLEGSDRQIELEIARNHIRMKSFDVTFTSKLIDGS
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCEEEEEECEEEEHHHHCCC
FPDYEGVIPIGADREVKVAREVLRDALQRAAILSNEKYRGVRIEVSPGQLKINAHNPEQE
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCHH
EAQEEIEAQTIVDGLAIGFNVNYLLDALSSLRGDFVNIQLRDSNSSALIRESNSEKSLQV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCEEE
VMPLRL
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]