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The map label for this gene is recF

Identifier: 182680575

GI number: 182680575

Start: 3044

End: 4138

Strand: Direct

Name: recF

Synonym: XfasM23_0003

Alternate gene names: 182680575

Gene position: 3044-4138 (Clockwise)

Preceding gene: 182680574

Following gene: 182680576

Centisome position: 0.12

GC content: 41.0

Gene sequence:

>1095_bases
ATGCACATCACTCAGCTCGTCTTACGTCATTTTCGTTGTTTTGATGTCGTTGATTTCTTTCCATTACCTGGACTGAATTT
TTTCATTGGTGAAAATGGTTCAGGTAAGACTAGTTTACTTGAAGCCGTACATTTAATGGGTTACGGGCGGAGCTTTCGCG
GCAGGGTCCGTGATGGTTTGATCCGACATGGCTCTGAAAATCTAGAAATTTTTGTGGATTGGCAAGAAACTGCTTTAATT
AATGCACGTCGACGTCGTGCAGGTTTAAGTCACTATGGGCAAGAATGGATAGGACGTTTAGATGGTCAAAAGATAATGCA
TTTGGCTAGTTTATGCGCTGCATTAGCAGTGATTACTTTTGAATCAAGTAGTTACCAACTCATCAATAGTAACGCCGAAT
TACGACGTCGGTTCTTGGATTGGGGATTATTCCATGTTGAACCAGATTTTCTGGATTTATGGCGTCGCTATACACATGTG
CTTAAACAACGTAATTCTTTACTTAAGCAGAAAGAAGAATTAGCCATGTTAGAAGCTTGGGATCAAAAATTGAGTGAAGT
TGGTGAACAATTGACTTTCCGTCGGTTTCAATATTTGGAGAGATTGAAACAACGAGTGATCCCTTTAATTTCAAGAATAA
CACCTAATTTAAAAATCCATGGTTTTAATTTTAATCATGGATGGCGTCGTCACGAATTGCCGTTGATTGATGCTTTATTT
ATTTCGCGTGAACGTGATTATCAGTATGGTTATACTTCATTAGGACCGCATCGTTCGGATTGGACTCCGCAATTTTCCAG
TATTCCTGGAGTTCATTTTTTATCTCGTGGACAAGGAAAACTTATAACATTGATGTGCCTACTAGCTCAGGCGCAGGACT
TTTTTGATCAGCGTGGGGAGTGGCCTATTCTTGCTTTAGATGACTTGGCTTCAGAATTGGATCAGAAACATCAATGGCGC
GTGTTAGAGATGCTAGCTGAAATTCCAGCCCAGGTCTTGATCACGGGGACAGAAATTCCCCAAGGACTCAAGCCTTTTTT
CTCAGTGGGTGCTATGTTTCACGTGGAACATGGCGCTATCACGCGAATGTTTTGA

Upstream 100 bases:

>100_bases
ATGGAATCTTAGTTGGGTATGTTATTATTTTTTATATTCTCATCATTCTTCCTAATTTCATTAGGTATGTTATCTATTGT
GCCAGTGAATTTTGAATCTG

Downstream 100 bases:

>100_bases
AGTGATTTTTAGATTGCATCGGTTACAGGCATTTAATTATTTTAAAATCCCCTTAACTTGAGGTGTTGATATTGAGGTGC
ATCTTCTCTGTTGATGTGTT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MHITQLVLRHFRCFDVVDFFPLPGLNFFIGENGSGKTSLLEAVHLMGYGRSFRGRVRDGLIRHGSENLEIFVDWQETALI
NARRRRAGLSHYGQEWIGRLDGQKIMHLASLCAALAVITFESSSYQLINSNAELRRRFLDWGLFHVEPDFLDLWRRYTHV
LKQRNSLLKQKEELAMLEAWDQKLSEVGEQLTFRRFQYLERLKQRVIPLISRITPNLKIHGFNFNHGWRRHELPLIDALF
ISRERDYQYGYTSLGPHRSDWTPQFSSIPGVHFLSRGQGKLITLMCLLAQAQDFFDQRGEWPILALDDLASELDQKHQWR
VLEMLAEIPAQVLITGTEIPQGLKPFFSVGAMFHVEHGAITRMF

Sequences:

>Translated_364_residues
MHITQLVLRHFRCFDVVDFFPLPGLNFFIGENGSGKTSLLEAVHLMGYGRSFRGRVRDGLIRHGSENLEIFVDWQETALI
NARRRRAGLSHYGQEWIGRLDGQKIMHLASLCAALAVITFESSSYQLINSNAELRRRFLDWGLFHVEPDFLDLWRRYTHV
LKQRNSLLKQKEELAMLEAWDQKLSEVGEQLTFRRFQYLERLKQRVIPLISRITPNLKIHGFNFNHGWRRHELPLIDALF
ISRERDYQYGYTSLGPHRSDWTPQFSSIPGVHFLSRGQGKLITLMCLLAQAQDFFDQRGEWPILALDDLASELDQKHQWR
VLEMLAEIPAQVLITGTEIPQGLKPFFSVGAMFHVEHGAITRMF
>Mature_364_residues
MHITQLVLRHFRCFDVVDFFPLPGLNFFIGENGSGKTSLLEAVHLMGYGRSFRGRVRDGLIRHGSENLEIFVDWQETALI
NARRRRAGLSHYGQEWIGRLDGQKIMHLASLCAALAVITFESSSYQLINSNAELRRRFLDWGLFHVEPDFLDLWRRYTHV
LKQRNSLLKQKEELAMLEAWDQKLSEVGEQLTFRRFQYLERLKQRVIPLISRITPNLKIHGFNFNHGWRRHELPLIDALF
ISRERDYQYGYTSLGPHRSDWTPQFSSIPGVHFLSRGQGKLITLMCLLAQAQDFFDQRGEWPILALDDLASELDQKHQWR
VLEMLAEIPAQVLITGTEIPQGLKPFFSVGAMFHVEHGAITRMF

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=365, Percent_Identity=33.972602739726, Blast_Score=184, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_XYLF2 (B2I5U9)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828735.1
- ProteinModelPortal:   B2I5U9
- SMR:   B2I5U9
- GeneID:   6204034
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0003
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42386; Mature: 42386

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITQLVLRHFRCFDVVDFFPLPGLNFFIGENGSGKTSLLEAVHLMGYGRSFRGRVRDGL
CCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IRHGSENLEIFVDWQETALINARRRRAGLSHYGQEWIGRLDGQKIMHLASLCAALAVITF
HHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEE
ESSSYQLINSNAELRRRFLDWGLFHVEPDFLDLWRRYTHVLKQRNSLLKQKEELAMLEAW
CCCCCEEECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQKLSEVGEQLTFRRFQYLERLKQRVIPLISRITPNLKIHGFNFNHGWRRHELPLIDALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
ISRERDYQYGYTSLGPHRSDWTPQFSSIPGVHFLSRGQGKLITLMCLLAQAQDFFDQRGE
HHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
WPILALDDLASELDQKHQWRVLEMLAEIPAQVLITGTEIPQGLKPFFSVGAMFHVEHGAI
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHCCCEEECCCCC
TRMF
CCCC
>Mature Secondary Structure
MHITQLVLRHFRCFDVVDFFPLPGLNFFIGENGSGKTSLLEAVHLMGYGRSFRGRVRDGL
CCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IRHGSENLEIFVDWQETALINARRRRAGLSHYGQEWIGRLDGQKIMHLASLCAALAVITF
HHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEE
ESSSYQLINSNAELRRRFLDWGLFHVEPDFLDLWRRYTHVLKQRNSLLKQKEELAMLEAW
CCCCCEEECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQKLSEVGEQLTFRRFQYLERLKQRVIPLISRITPNLKIHGFNFNHGWRRHELPLIDALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
ISRERDYQYGYTSLGPHRSDWTPQFSSIPGVHFLSRGQGKLITLMCLLAQAQDFFDQRGE
HHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
WPILALDDLASELDQKHQWRVLEMLAEIPAQVLITGTEIPQGLKPFFSVGAMFHVEHGAI
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHCCCEEECCCCC
TRMF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA