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The map label for this gene is pykA

Identifier: 170757030

GI number: 170757030

Start: 3676188

End: 3677945

Strand: Reverse

Name: pykA

Synonym: CLD_1136

Alternate gene names: 170757030

Gene position: 3677945-3676188 (Counterclockwise)

Preceding gene: 170757584

Following gene: 170756892

Centisome position: 92.92

GC content: 32.54

Gene sequence:

>1758_bases
ATGCAAAAGACTAAAATGATATTTACAATAGGCCCTGCAAGCTCGACAGAAGAAATTGTATCAAAATTAATAGAAGCTGG
AATGAGTGTATCAAGACACAATTTTTCTCACGGATCACATCCAGAACACAAAGAAAGAATGATGATGATTAAGAAATTAA
GAGAAAAACATAACAAACACATAGCTATTATGTTAGACACCAAAGGACCAGAAATAAGAACAGGTAATTTTTCAGTGGAT
AAAGTGGAATTAAAAGAAGGGGCAGAGTTTACAATATATTGTAGAGAAGACATAGTAGGTGATGAAACAAAATGTTCTAT
TACTTATGATGAACTATATAAAGATGTAGTTAAAGGAAATAAAATACTGATAGATGATGGATTAGTAGAGCTAGAGGTTC
AGTCAGTAGAAGATAATAAAATCCATACAGTAGTTAAAAATTCAGGAACTGTTTCAAATCACAAGGGAGTAAATGTTCCA
GGAGTTTCAGTATCACTTCCAGCAGTTACTGAAAAAGATATAGAAGATTTAAAATTTGGATGTGAAGTAGGAGTAGATTT
AATAAGTGCATCTTTTATAAGAAAAGCCTCTGATGTATTAGCTATAAGAAAAATATTGGAAGAAAATGGTGGAAATGAAA
TACAGATAATCTCTAAGATAGAAAACCAAGAGGGCGTTGACAATATAGATGAGATAATAAAATTTTCTGATGGAATAATG
GTAGCTAGAGGAGATATGGGAGTCGAAATTCCTATAGAAGAAGTTCCAATAGTACAAAAAAGAATAATAGAAAAATGTAA
TAAGGCAGGTAAGCCTGTAATTACAGCAACTCAAATGTTAGATTCTATGATGAGAAATCCTAGACCAACAAGAGCTGAAG
CCTCAGACATAGCCAATGCAATATTTGATGGAACAGATGCCATAATGTTAAGTGGAGAATCAGCAAATGGAAAATATCCA
GTAGAAGCTGCAAGAACTATGTCTAGAATAGCTAAAACAGCAGAAGCAAAATTAAATTATGATGCTATATTAAATAAAAT
GAGAGAAAGTCATATATTAAATGTTCCTAATGCTATAAGCTTATCAGCGTGTACTACAGCATCAGAATTAAATGCTACAG
CTATAATCACAGCAACTCAAAGTGGGCATACTGCAAAAATGGTATCAAAATATAGACCACAATGCCCTATTATAGCAGTA
ACACCAAATGAAATAGTTGCTAGAAAGTTAGCATTAAATTGTGGAGTAGTACCACTATTAACGGAAACATTTAATTCTAC
AGATGAGCTTATAGATAAATCAGTTAATAAATCTTTAGAAGAAGGATATGTTAAAAATGGCGACTTAGTTGTCATAGCAG
GAGGAATACCAGTGTCTTACTCAGGAACAACTAATATGCTAAAAGTTCACATAGTAGGAGATATCTTAGCCCAAGGAAGA
GGTTCAGGAACTAGACCAACTTATGGGAATGTAAAGATAGTAAACAATCCTTCAGAAGCAGAAGATATAGTTGAAAGAGA
TGACATATTAGTAGTTAAAAATTTAGATAGAAGATATATGACTATTTTAGATAGAGTATCAGGCATAGTGGCTGAAGAAG
GTGGAATAACATCACACTTAGCTATAGAATGCATAGCAAGGGATATTCCATTTATATGTAATGCAGGTGGAGCTATGGAT
GTTTTAAAAACAGGAACATATGTAACACTTGATACAGTAAGAGGAATAGTATACAACGGAAGAGCTAATATAGTTTAA

Upstream 100 bases:

>100_bases
AGCTTTAGCAGTAGAAAGAAGTTTTGACCAAGAATTATATGACATAGCAACTATTTTATCCAAATAATGTAAAAGTTTAG
ATTTTGAGGAGGCGTTTTAT

Downstream 100 bases:

>100_bases
ATATAATAAAAATTATATACTAAAATAAAGAGTCCATTTAGAAAAATCAAACTTTCTATTCGGGCTCTTAAATTTTATTT
TCTTGACACCAAAGTATTAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKHIAIMLDTKGPEIRTGNFSVD
KVELKEGAEFTIYCREDIVGDETKCSITYDELYKDVVKGNKILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVP
GVSVSLPAVTEKDIEDLKFGCEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANAIFDGTDAIMLSGESANGKYP
VEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAISLSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAV
TPNEIVARKLALNCGVVPLLTETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILAQGR
GSGTRPTYGNVKIVNNPSEAEDIVERDDILVVKNLDRRYMTILDRVSGIVAEEGGITSHLAIECIARDIPFICNAGGAMD
VLKTGTYVTLDTVRGIVYNGRANIV

Sequences:

>Translated_585_residues
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKHIAIMLDTKGPEIRTGNFSVD
KVELKEGAEFTIYCREDIVGDETKCSITYDELYKDVVKGNKILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVP
GVSVSLPAVTEKDIEDLKFGCEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANAIFDGTDAIMLSGESANGKYP
VEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAISLSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAV
TPNEIVARKLALNCGVVPLLTETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILAQGR
GSGTRPTYGNVKIVNNPSEAEDIVERDDILVVKNLDRRYMTILDRVSGIVAEEGGITSHLAIECIARDIPFICNAGGAMD
VLKTGTYVTLDTVRGIVYNGRANIV
>Mature_585_residues
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKHIAIMLDTKGPEIRTGNFSVD
KVELKEGAEFTIYCREDIVGDETKCSITYDELYKDVVKGNKILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVP
GVSVSLPAVTEKDIEDLKFGCEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANAIFDGTDAIMLSGESANGKYP
VEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAISLSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAV
TPNEIVARKLALNCGVVPLLTETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILAQGR
GSGTRPTYGNVKIVNNPSEAEDIVERDDILVVKNLDRRYMTILDRVSGIVAEEGGITSHLAIECIARDIPFICNAGGAMD
VLKTGTYVTLDTVRGIVYNGRANIV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=45.5852156057495, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=45.5852156057495, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=45.9958932238193, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=43.558282208589, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=43.558282208589, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=49.3055555555556, Blast_Score=249, Evalue=7e-66,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=49.3055555555556, Blast_Score=249, Evalue=7e-66,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=46.6101694915254, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=46.6101694915254, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=46.9767441860465, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=49.8942917547569, Blast_Score=457, Evalue=1e-129,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=39.622641509434, Blast_Score=314, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=44.7422680412371, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=42.3868312757202, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=42.3868312757202, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=42.3868312757202, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=42.3868312757202, Blast_Score=342, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6319279, Length=489, Percent_Identity=43.9672801635992, Blast_Score=353, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6324923, Length=494, Percent_Identity=40.8906882591093, Blast_Score=346, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24648964, Length=482, Percent_Identity=44.8132780082988, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28571814, Length=482, Percent_Identity=44.8132780082988, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24648966, Length=420, Percent_Identity=47.8571428571429, Blast_Score=354, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=35.1351351351351, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24646914, Length=286, Percent_Identity=47.5524475524476, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 63650; Mature: 63650

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKH
CCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCE
IAIMLDTKGPEIRTGNFSVDKVELKEGAEFTIYCREDIVGDETKCSITYDELYKDVVKGN
EEEEEECCCCEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCC
KILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVPGVSVSLPAVTEKDIEDLKFG
EEEEECCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHH
CEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEE
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANA
EEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHH
IFDGTDAIMLSGESANGKYPVEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAIS
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEE
LSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAVTPNEIVARKLALNCGVVPLL
EHHHCCHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCHHHH
TETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILAQGR
HHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCEEEEEEEEHHHHCCC
GSGTRPTYGNVKIVNNPSEAEDIVERDDILVVKNLDRRYMTILDRVSGIVAEEGGITSHL
CCCCCCCCCCEEEECCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHEECCCCCCHHH
AIECIARDIPFICNAGGAMDVLKTGTYVTLDTVRGIVYNGRANIV
HHHHHHHCCCEEECCCCCHHHHHCCCEEEEECCCCEEECCCCCCC
>Mature Secondary Structure
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKH
CCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCE
IAIMLDTKGPEIRTGNFSVDKVELKEGAEFTIYCREDIVGDETKCSITYDELYKDVVKGN
EEEEEECCCCEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCC
KILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVPGVSVSLPAVTEKDIEDLKFG
EEEEECCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHH
CEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEE
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANA
EEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHH
IFDGTDAIMLSGESANGKYPVEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAIS
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEE
LSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAVTPNEIVARKLALNCGVVPLL
EHHHCCHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCHHHH
TETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILAQGR
HHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCEEEEEEEEHHHHCCC
GSGTRPTYGNVKIVNNPSEAEDIVERDDILVVKNLDRRYMTILDRVSGIVAEEGGITSHL
CCCCCCCCCCEEEECCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHEECCCCCCHHH
AIECIARDIPFICNAGGAMDVLKTGTYVTLDTVRGIVYNGRANIV
HHHHHHHCCCEEECCCCCHHHHHCCCEEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286; 9625784 [H]