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The map label for this gene is pfkA

Identifier: 170757584

GI number: 170757584

Start: 3677979

End: 3678938

Strand: Reverse

Name: pfkA

Synonym: CLD_1135

Alternate gene names: 170757584

Gene position: 3678938-3677979 (Counterclockwise)

Preceding gene: 170756612

Following gene: 170757030

Centisome position: 92.94

GC content: 32.5

Gene sequence:

>960_bases
ATGAGAACTATAGCTGTATTAACAAGTGGTGGAGATGCACCAGGAATGAATGCTGCTATCAGAGCAGTAGTTAGAACAGG
TTTAGAAAAAGGTTTAAAAGTAATGGGAATTCAAAGAGGATATAACGGACTTATAAATGGAGAAATATTTGAAATGGATA
CTCATAGCGTATCTGATATAATACAAAGAGGCGGAACTATATTAAGAACAGCTCGTTGTGAAGAATTTAGAACAGAGCAG
GGAAGAGAAAAAGCTGCTAAAATATTAAAAGCCTTTGGTATTGATGGATTAGTTGTAATTGGTGGAGACGGTTCTTTTCA
TGGGGCCCAATTATTATCTAAGTTAGGAATAAATACTGTTGGACTACCAGGTACTATAGATAATGATTTAGCTTATACAG
ATTATACAATTGGCTTTGATACATCTATAAATACAGTTTTAGATGCTATAAATAAGCTTAGAGATACATCCACTTCTCAT
GAAAGAGTAAGTGTAGTAGAAGTTATGGGAAGAAATTGTGGAGATATAGCATTATATACAGGAGTAGCTGGTGGAGCAGA
AAGTATTATAATACCAGAAAAAGAGTATAATGCAGATAAATTATGCAAACAAATACTACAAGGAAAATTAAAAGGGAAAA
TGCATAACTTAGTTTTATTAGCAGAAGGTGTTGGAGGAGCTAATGAACTAGCAAAATATATAGAAGAGGTTACAGGGATA
GAAACAAGATCTACAATATTAGGACATATACAAAGGGGTGGAAGTCCAACTTGTATGGATAGAATATTAGCTTCAAGAAT
GGCTTATAAGGCAGTAGAATTATTGATTAGTGGTAAATCTTCAAGAGTAGTTGGTATAAAAAATGGAAAAATAATTGATA
TGGATATAGATGAAGCTTTAGCAGTAGAAAGAAGTTTTGACCAAGAATTATATGACATAGCAACTATTTTATCCAAATAA

Upstream 100 bases:

>100_bases
AGTTATGTTATAATTGAAAATATACAAAGCAGAATATAATTAGGTTTATAATATAAGTGGGACTACAAATGTCCCAGTGG
TCGTAGGAGGTATTTATCAT

Downstream 100 bases:

>100_bases
TGTAAAAGTTTAGATTTTGAGGAGGCGTTTTATATGCAAAAGACTAAAATGATATTTACAATAGGCCCTGCAAGCTCGAC
AGAAGAAATTGTATCAAAAT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRTIAVLTSGGDAPGMNAAIRAVVRTGLEKGLKVMGIQRGYNGLINGEIFEMDTHSVSDIIQRGGTILRTARCEEFRTEQ
GREKAAKILKAFGIDGLVVIGGDGSFHGAQLLSKLGINTVGLPGTIDNDLAYTDYTIGFDTSINTVLDAINKLRDTSTSH
ERVSVVEVMGRNCGDIALYTGVAGGAESIIIPEKEYNADKLCKQILQGKLKGKMHNLVLLAEGVGGANELAKYIEEVTGI
ETRSTILGHIQRGGSPTCMDRILASRMAYKAVELLISGKSSRVVGIKNGKIIDMDIDEALAVERSFDQELYDIATILSK

Sequences:

>Translated_319_residues
MRTIAVLTSGGDAPGMNAAIRAVVRTGLEKGLKVMGIQRGYNGLINGEIFEMDTHSVSDIIQRGGTILRTARCEEFRTEQ
GREKAAKILKAFGIDGLVVIGGDGSFHGAQLLSKLGINTVGLPGTIDNDLAYTDYTIGFDTSINTVLDAINKLRDTSTSH
ERVSVVEVMGRNCGDIALYTGVAGGAESIIIPEKEYNADKLCKQILQGKLKGKMHNLVLLAEGVGGANELAKYIEEVTGI
ETRSTILGHIQRGGSPTCMDRILASRMAYKAVELLISGKSSRVVGIKNGKIIDMDIDEALAVERSFDQELYDIATILSK
>Mature_319_residues
MRTIAVLTSGGDAPGMNAAIRAVVRTGLEKGLKVMGIQRGYNGLINGEIFEMDTHSVSDIIQRGGTILRTARCEEFRTEQ
GREKAAKILKAFGIDGLVVIGGDGSFHGAQLLSKLGINTVGLPGTIDNDLAYTDYTIGFDTSINTVLDAINKLRDTSTSH
ERVSVVEVMGRNCGDIALYTGVAGGAESIIIPEKEYNADKLCKQILQGKLKGKMHNLVLLAEGVGGANELAKYIEEVTGI
ETRSTILGHIQRGGSPTCMDRILASRMAYKAVELLISGKSSRVVGIKNGKIIDMDIDEALAVERSFDQELYDIATILSK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453619, Length=356, Percent_Identity=38.7640449438202, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI266453768, Length=356, Percent_Identity=38.7640449438202, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI266453748, Length=356, Percent_Identity=38.7640449438202, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI4505749, Length=356, Percent_Identity=38.7640449438202, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI11321601, Length=358, Percent_Identity=39.1061452513966, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI48762920, Length=358, Percent_Identity=37.9888268156425, Blast_Score=221, Evalue=5e-58,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=53.7735849056604, Blast_Score=332, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=39.4230769230769, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25147584, Length=310, Percent_Identity=41.2903225806452, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI72003601, Length=339, Percent_Identity=27.1386430678466, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321679, Length=343, Percent_Identity=39.067055393586, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=344, Percent_Identity=35.4651162790698, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24652337, Length=334, Percent_Identity=41.6167664670659, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17647809, Length=334, Percent_Identity=41.6167664670659, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI28573326, Length=334, Percent_Identity=39.8203592814371, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_CLOB1 (A7FYW7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385690.1
- ProteinModelPortal:   A7FYW7
- SMR:   A7FYW7
- STRING:   A7FYW7
- GeneID:   5395214
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3429
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- ProtClustDB:   PRK03202
- BioCyc:   CBOT441770:CLB_3429-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34204; Mature: 34204

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 243-243 BINDING 249-249 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIAVLTSGGDAPGMNAAIRAVVRTGLEKGLKVMGIQRGYNGLINGEIFEMDTHSVSDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCEEEECCCHHHHHH
IQRGGTILRTARCEEFRTEQGREKAAKILKAFGIDGLVVIGGDGSFHGAQLLSKLGINTV
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCC
GLPGTIDNDLAYTDYTIGFDTSINTVLDAINKLRDTSTSHERVSVVEVMGRNCGDIALYT
CCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEE
GVAGGAESIIIPEKEYNADKLCKQILQGKLKGKMHNLVLLAEGVGGANELAKYIEEVTGI
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCC
ETRSTILGHIQRGGSPTCMDRILASRMAYKAVELLISGKSSRVVGIKNGKIIDMDIDEAL
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHH
AVERSFDQELYDIATILSK
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTIAVLTSGGDAPGMNAAIRAVVRTGLEKGLKVMGIQRGYNGLINGEIFEMDTHSVSDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCEEEECCCHHHHHH
IQRGGTILRTARCEEFRTEQGREKAAKILKAFGIDGLVVIGGDGSFHGAQLLSKLGINTV
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCC
GLPGTIDNDLAYTDYTIGFDTSINTVLDAINKLRDTSTSHERVSVVEVMGRNCGDIALYT
CCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEE
GVAGGAESIIIPEKEYNADKLCKQILQGKLKGKMHNLVLLAEGVGGANELAKYIEEVTGI
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCC
ETRSTILGHIQRGGSPTCMDRILASRMAYKAVELLISGKSSRVVGIKNGKIIDMDIDEAL
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHH
AVERSFDQELYDIATILSK
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA