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The map label for this gene is citA [H]

Identifier: 170751471

GI number: 170751471

Start: 5410648

End: 5411955

Strand: Direct

Name: citA [H]

Synonym: Mrad2831_5091

Alternate gene names: 170751471

Gene position: 5410648-5411955 (Clockwise)

Preceding gene: 170751470

Following gene: 170751473

Centisome position: 89.02

GC content: 67.28

Gene sequence:

>1308_bases
ATGGCCGGTGATCCCGGGGCCGTCGGGGCCACGCTCCAGTCGAGGATCGGCGCGATCCTCCGCTCGACGAGCGGCAACTT
CCTGGAGATGTTCGACTTCTTTCTGTTCGGGTTCTACGCCACCTACATCGCGCGGGCGTTCTTCCCCTCGGGCAACGAGA
CCACCGAGCTGCTCCTAACCTTCGGCACGTTCTGGCTCGGGGCGCTGATGCGGCCGCTCGGGGCGATCGTGCTGGGCGCC
TATATCGACCGGATCGGTCGCCGGAAGGGCCTGATCATCACGCTCGCCATCATGGCGAGCGGCACGGTGCTCATCGCCTT
CTGCCCGAGCTACGACACGATCGGCGTCGCGGCCCCGCTGATCGTCCTCGCCGGGCGCCTGCTGCAGGGCTTCTCGGCGG
GCGTCGAGATCGGCGGCGTCTCGATCTACCTGTTCGAGATCGCCACGCCCGGTCGGCGCGGCTTCTACACGTCGTTCCAG
TCGGCGAGCCAGCAGGTGGCGATCATGTTCGCCGCCCTGATCGGCTACGGGCTGAACGCCTTCCTGACCAAGCAGCAGAT
CGGCGATTTCGGCTGGCGCATCCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCATCTTCTTCATCCGGCGCTCGCTCC
AAGAGACGCAGGACTTCGCCAAGCGGACCCACCATCCGAGCACCCGCGAGATCCTGACCACGGTGGCGGCCAACTGGTGG
ATCGTCCTGCTCGGCATGATGCTGACCACGATGACGACGGTGACCTTCTACATGATCACCGTCTACACCCCGACCTTCGG
CAGGAACGTCCTCAAGCTCACCGAGACCGACAGCCTGATCGTCACGCTGTTCGTGGCGATCACGAACTTCATCTGGCTGC
CCGTGGGCGGGGCCCTGTCGGACGCCGTCGGCCGGAAGCCGGTGCTGCTGACGATCTCGACCCTGTCGCTGCTGACGACC
TACCCGGCCCTGCACTGGCTCGTGGCCGACCCGACCTTCGGCAAGATGCTCGCCGTCGAGCTGTGGTTCTCGTTCTTCTT
CGGCGTCTACAACGGCGCGATGGTGGTCTCCCTCTCCGAGGTGGTGCCGCCGCACGTCCGCGCGAGCGGCTTCTCGCTGG
CCTACAGCCTCGCCACCGCGCTGTTCGGCACGGCCACGCCGATGGTCTCGACCTTCCTGATCGAGAAGACCGGCGACCGG
GCGGCGCCGAGCTACTGGCTGATGGCGGCGGCCGCCTGCGGCATCGTCGCGACGCTCGCCCTGTTCCGGGGCCGGGCGGG
ACTGGAGCGCGTGCCAGCCCACGCCTGA

Upstream 100 bases:

>100_bases
GCAGATCGCCACCCGCATCGGCGGCGCGCTGACCGTGACCGGCACGATCGTCGCCATGAAGTGGATCGGTGCCTGATGGG
CACCCGGAAAGGACGTCGTC

Downstream 100 bases:

>100_bases
GGGCCGGGGCCGCTCAGCTCTCCTGCCAGATCCGGATCCGCGCCTTGAAGGCGGCGCGGATATGGGCCCGGGCGGCGGCC
TCGGCGGCATCCCCGTCGTG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAGDPGAVGATLQSRIGAILRSTSGNFLEMFDFFLFGFYATYIARAFFPSGNETTELLLTFGTFWLGALMRPLGAIVLGA
YIDRIGRRKGLIITLAIMASGTVLIAFCPSYDTIGVAAPLIVLAGRLLQGFSAGVEIGGVSIYLFEIATPGRRGFYTSFQ
SASQQVAIMFAALIGYGLNAFLTKQQIGDFGWRIPFLIGCLIVPFIFFIRRSLQETQDFAKRTHHPSTREILTTVAANWW
IVLLGMMLTTMTTVTFYMITVYTPTFGRNVLKLTETDSLIVTLFVAITNFIWLPVGGALSDAVGRKPVLLTISTLSLLTT
YPALHWLVADPTFGKMLAVELWFSFFFGVYNGAMVVSLSEVVPPHVRASGFSLAYSLATALFGTATPMVSTFLIEKTGDR
AAPSYWLMAAAACGIVATLALFRGRAGLERVPAHA

Sequences:

>Translated_435_residues
MAGDPGAVGATLQSRIGAILRSTSGNFLEMFDFFLFGFYATYIARAFFPSGNETTELLLTFGTFWLGALMRPLGAIVLGA
YIDRIGRRKGLIITLAIMASGTVLIAFCPSYDTIGVAAPLIVLAGRLLQGFSAGVEIGGVSIYLFEIATPGRRGFYTSFQ
SASQQVAIMFAALIGYGLNAFLTKQQIGDFGWRIPFLIGCLIVPFIFFIRRSLQETQDFAKRTHHPSTREILTTVAANWW
IVLLGMMLTTMTTVTFYMITVYTPTFGRNVLKLTETDSLIVTLFVAITNFIWLPVGGALSDAVGRKPVLLTISTLSLLTT
YPALHWLVADPTFGKMLAVELWFSFFFGVYNGAMVVSLSEVVPPHVRASGFSLAYSLATALFGTATPMVSTFLIEKTGDR
AAPSYWLMAAAACGIVATLALFRGRAGLERVPAHA
>Mature_434_residues
AGDPGAVGATLQSRIGAILRSTSGNFLEMFDFFLFGFYATYIARAFFPSGNETTELLLTFGTFWLGALMRPLGAIVLGAY
IDRIGRRKGLIITLAIMASGTVLIAFCPSYDTIGVAAPLIVLAGRLLQGFSAGVEIGGVSIYLFEIATPGRRGFYTSFQS
ASQQVAIMFAALIGYGLNAFLTKQQIGDFGWRIPFLIGCLIVPFIFFIRRSLQETQDFAKRTHHPSTREILTTVAANWWI
VLLGMMLTTMTTVTFYMITVYTPTFGRNVLKLTETDSLIVTLFVAITNFIWLPVGGALSDAVGRKPVLLTISTLSLLTTY
PALHWLVADPTFGKMLAVELWFSFFFGVYNGAMVVSLSEVVPPHVRASGFSLAYSLATALFGTATPMVSTFLIEKTGDRA
APSYWLMAAAACGIVATLALFRGRAGLERVPAHA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=410, Percent_Identity=30.4878048780488, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=32.4825986078886, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1788292, Length=429, Percent_Identity=27.2727272727273, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=30.4038004750594, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082231, Length=212, Percent_Identity=26.8867924528302, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47196; Mature: 47065

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGDPGAVGATLQSRIGAILRSTSGNFLEMFDFFLFGFYATYIARAFFPSGNETTELLLT
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FGTFWLGALMRPLGAIVLGAYIDRIGRRKGLIITLAIMASGTVLIAFCPSYDTIGVAAPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCEEEEECCCCCCHHHHHHH
IVLAGRLLQGFSAGVEIGGVSIYLFEIATPGRRGFYTSFQSASQQVAIMFAALIGYGLNA
HHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
FLTKQQIGDFGWRIPFLIGCLIVPFIFFIRRSLQETQDFAKRTHHPSTREILTTVAANWW
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IVLLGMMLTTMTTVTFYMITVYTPTFGRNVLKLTETDSLIVTLFVAITNFIWLPVGGALS
HHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHH
DAVGRKPVLLTISTLSLLTTYPALHWLVADPTFGKMLAVELWFSFFFGVYNGAMVVSLSE
HHCCCCCEEEEHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCHHEEEEHH
VVPPHVRASGFSLAYSLATALFGTATPMVSTFLIEKTGDRAAPSYWLMAAAACGIVATLA
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LFRGRAGLERVPAHA
HHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGDPGAVGATLQSRIGAILRSTSGNFLEMFDFFLFGFYATYIARAFFPSGNETTELLLT
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FGTFWLGALMRPLGAIVLGAYIDRIGRRKGLIITLAIMASGTVLIAFCPSYDTIGVAAPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCEEEEECCCCCCHHHHHHH
IVLAGRLLQGFSAGVEIGGVSIYLFEIATPGRRGFYTSFQSASQQVAIMFAALIGYGLNA
HHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
FLTKQQIGDFGWRIPFLIGCLIVPFIFFIRRSLQETQDFAKRTHHPSTREILTTVAANWW
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IVLLGMMLTTMTTVTFYMITVYTPTFGRNVLKLTETDSLIVTLFVAITNFIWLPVGGALS
HHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHH
DAVGRKPVLLTISTLSLLTTYPALHWLVADPTFGKMLAVELWFSFFFGVYNGAMVVSLSE
HHCCCCCEEEEHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCHHEEEEHH
VVPPHVRASGFSLAYSLATALFGTATPMVSTFLIEKTGDRAAPSYWLMAAAACGIVATLA
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LFRGRAGLERVPAHA
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]