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The map label for this gene is fumC [H]

Identifier: 170751248

GI number: 170751248

Start: 5195145

End: 5196602

Strand: Reverse

Name: fumC [H]

Synonym: Mrad2831_4865

Alternate gene names: 170751248

Gene position: 5196602-5195145 (Counterclockwise)

Preceding gene: 170751249

Following gene: 170751247

Centisome position: 85.5

GC content: 69.89

Gene sequence:

>1458_bases
ATGAACGCGCCCGTCCCGGACCATCCCGTCCTGCGCGATGTCCCGATCGGCATCTCCGCCACGGGGATGCGGCGGGAGAC
GGATTCGATGGGCGGGATCGACGTTCCGGCGGACCGCTACTGGGGCGCGCAGACCCAGCGCTCGCTCCAGCATTTCTCGA
TCGGCAACGATCGCATGCCGAAGCGGGTCTACCACGCCTACGGCTACGTCAAGAAGGCCGCCGCCCTGGTCAACGCCGCC
GCCGGGCGGCTGGAGGATTGGCGCGCGCAGGCCATCGTCCAGGCCGCCGACGAGGCGATCGCGGGGAAGCTCGACGCGAA
CTTCCCGCTCTACGTCTGGCAGACCGGTTCCGGCACCCAGTCCAACATGAACGTCAACGAGGTGCTGTCGAACCGCGCGA
TCCAGCTCCTCGGCGGTGAACTCGGCTCCAAGAGCCCGGTCCATCCGAACGACCACGTCAACATGGGCCAGTCGTCGAAC
GACACCTTCCCGACGGCGATGCACATCGCCACGGTGCTCGAACTCGACGAGGTCCTGCTCCCGCAGGCGCGGGCGCTGGC
CGACGCGATCGACGCCAAGGCGCGGGCCTGGATGGACGTGGTCAAGACCGGAAGGACCCACCTCCAGGACGCGACGCCCC
TCACGGTCGGCCAGGAATGGTCGGGCTACGTGGAGCAGATGCGCGACGCGCTGGCCCTGGTCGAGGCGTCGCGCGCCGGG
CTGTACAAGCTCGCGGCCGGCGGCACCGCGGTCGGCACCGGCCTCAACGCCCCGGAGGGCTTCAGCGCCGAGATCGCCGC
CAAGATCGCCGACCTCACCGGCAGGCCGTTCGTCACGGCGCCGAACAAGTTCGCCGCGCAGGGGTCGCTGGACGCCATGG
TCGCCGCCATGGCGGCGGTGCGCGGGCTCGCCGTCGCGCTGATGAAGATCGCCAACGACATGCGCTGGCTCGCCTCGGGG
CCGCGCTGCGGGCTCGGCGAGCTGCTGCTGCCGGCCAACGAGCCGGGCTCCTCGATCATGCCCGGCAAGGTCAACCCGAC
CCAGTGCGAGGCCATGGTGATGGTCTGCATCCAGGTGATCGGCGAGGACAACGCGGTCGCCTTCGCGGGGGCGCAGGGCA
ATTTCGAGCTGAACGCGATGCGCCCGATCATCATCAACAACACGCTGCACGCGATCCGCGTGCTGGGCGATGCCTGCGAG
AAGTTCCGGACCTACTCGGTCGCGGGCACGGAGCTCAACCGGAAGCGGATCGACGAGACCGTGAACCAGTCCCTGATGCT
GGTCACGGCCCTGTCGCCGGTGATCGGCTACGACCTCGCCTCGAAGATCGCCCACCGCGCCAACGACGAGGGCACGACGC
TGCGCGCGGCCGCCCTGGCCGAGGGCGTTCCGGCCGAGCGCTTCGACGCGGTGTGCGATCCCAAGACCATGATCGGCCAC
GGGCTGGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
AGAAAGCAGAGAAAACCGCCACCGTTTACGTCAAAGCGCACCCGGTTCGCCGACCCCGTCTTGCACTGTGCCACACGGTT
CGATGCCGAGAAGGATGACG

Downstream 100 bases:

>100_bases
CCCGCCGGGACCGTCCGTCCTGCCCCCTCAGCTGAGCGAGACACCATGAGCACGAAGGTCGCCGATCTGTTCGTCCGGGC
GCTGGCGCAGGCCGGCGTGA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNAPVPDHPVLRDVPIGISATGMRRETDSMGGIDVPADRYWGAQTQRSLQHFSIGNDRMPKRVYHAYGYVKKAAALVNAA
AGRLEDWRAQAIVQAADEAIAGKLDANFPLYVWQTGSGTQSNMNVNEVLSNRAIQLLGGELGSKSPVHPNDHVNMGQSSN
DTFPTAMHIATVLELDEVLLPQARALADAIDAKARAWMDVVKTGRTHLQDATPLTVGQEWSGYVEQMRDALALVEASRAG
LYKLAAGGTAVGTGLNAPEGFSAEIAAKIADLTGRPFVTAPNKFAAQGSLDAMVAAMAAVRGLAVALMKIANDMRWLASG
PRCGLGELLLPANEPGSSIMPGKVNPTQCEAMVMVCIQVIGEDNAVAFAGAQGNFELNAMRPIIINNTLHAIRVLGDACE
KFRTYSVAGTELNRKRIDETVNQSLMLVTALSPVIGYDLASKIAHRANDEGTTLRAAALAEGVPAERFDAVCDPKTMIGH
GLAGA

Sequences:

>Translated_485_residues
MNAPVPDHPVLRDVPIGISATGMRRETDSMGGIDVPADRYWGAQTQRSLQHFSIGNDRMPKRVYHAYGYVKKAAALVNAA
AGRLEDWRAQAIVQAADEAIAGKLDANFPLYVWQTGSGTQSNMNVNEVLSNRAIQLLGGELGSKSPVHPNDHVNMGQSSN
DTFPTAMHIATVLELDEVLLPQARALADAIDAKARAWMDVVKTGRTHLQDATPLTVGQEWSGYVEQMRDALALVEASRAG
LYKLAAGGTAVGTGLNAPEGFSAEIAAKIADLTGRPFVTAPNKFAAQGSLDAMVAAMAAVRGLAVALMKIANDMRWLASG
PRCGLGELLLPANEPGSSIMPGKVNPTQCEAMVMVCIQVIGEDNAVAFAGAQGNFELNAMRPIIINNTLHAIRVLGDACE
KFRTYSVAGTELNRKRIDETVNQSLMLVTALSPVIGYDLASKIAHRANDEGTTLRAAALAEGVPAERFDAVCDPKTMIGH
GLAGA
>Mature_485_residues
MNAPVPDHPVLRDVPIGISATGMRRETDSMGGIDVPADRYWGAQTQRSLQHFSIGNDRMPKRVYHAYGYVKKAAALVNAA
AGRLEDWRAQAIVQAADEAIAGKLDANFPLYVWQTGSGTQSNMNVNEVLSNRAIQLLGGELGSKSPVHPNDHVNMGQSSN
DTFPTAMHIATVLELDEVLLPQARALADAIDAKARAWMDVVKTGRTHLQDATPLTVGQEWSGYVEQMRDALALVEASRAG
LYKLAAGGTAVGTGLNAPEGFSAEIAAKIADLTGRPFVTAPNKFAAQGSLDAMVAAMAAVRGLAVALMKIANDMRWLASG
PRCGLGELLLPANEPGSSIMPGKVNPTQCEAMVMVCIQVIGEDNAVAFAGAQGNFELNAMRPIIINNTLHAIRVLGDACE
KFRTYSVAGTELNRKRIDETVNQSLMLVTALSPVIGYDLASKIAHRANDEGTTLRAAALAEGVPAERFDAVCDPKTMIGH
GLAGA

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=464, Percent_Identity=57.9741379310345, Blast_Score=523, Evalue=1e-148,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=57.0194384449244, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI87082375, Length=450, Percent_Identity=36.6666666666667, Blast_Score=288, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=57.1739130434783, Blast_Score=528, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=53.680981595092, Blast_Score=351, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=55.8441558441558, Blast_Score=522, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=56.3991323210412, Blast_Score=515, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24640177, Length=466, Percent_Identity=55.793991416309, Blast_Score=514, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24662684, Length=460, Percent_Identity=53.9130434782609, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=53.5791757049892, Blast_Score=506, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24583245, Length=460, Percent_Identity=49.3478260869565, Blast_Score=460, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 51427; Mature: 51427

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVPDHPVLRDVPIGISATGMRRETDSMGGIDVPADRYWGAQTQRSLQHFSIGNDRMP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCH
KRVYHAYGYVKKAAALVNAAAGRLEDWRAQAIVQAADEAIAGKLDANFPLYVWQTGSGTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
SNMNVNEVLSNRAIQLLGGELGSKSPVHPNDHVNMGQSSNDTFPTAMHIATVLELDEVLL
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PQARALADAIDAKARAWMDVVKTGRTHLQDATPLTVGQEWSGYVEQMRDALALVEASRAG
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LYKLAAGGTAVGTGLNAPEGFSAEIAAKIADLTGRPFVTAPNKFAAQGSLDAMVAAMAAV
EEEEECCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHH
RGLAVALMKIANDMRWLASGPRCGLGELLLPANEPGSSIMPGKVNPTQCEAMVMVCIQVI
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GEDNAVAFAGAQGNFELNAMRPIIINNTLHAIRVLGDACEKFRTYSVAGTELNRKRIDET
CCCCEEEEECCCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHH
VNQSLMLVTALSPVIGYDLASKIAHRANDEGTTLRAAALAEGVPAERFDAVCDPKTMIGH
HHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCHHHHHHHCCCHHHHCC
GLAGA
CCCCC
>Mature Secondary Structure
MNAPVPDHPVLRDVPIGISATGMRRETDSMGGIDVPADRYWGAQTQRSLQHFSIGNDRMP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCH
KRVYHAYGYVKKAAALVNAAAGRLEDWRAQAIVQAADEAIAGKLDANFPLYVWQTGSGTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
SNMNVNEVLSNRAIQLLGGELGSKSPVHPNDHVNMGQSSNDTFPTAMHIATVLELDEVLL
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PQARALADAIDAKARAWMDVVKTGRTHLQDATPLTVGQEWSGYVEQMRDALALVEASRAG
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LYKLAAGGTAVGTGLNAPEGFSAEIAAKIADLTGRPFVTAPNKFAAQGSLDAMVAAMAAV
EEEEECCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHH
RGLAVALMKIANDMRWLASGPRCGLGELLLPANEPGSSIMPGKVNPTQCEAMVMVCIQVI
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GEDNAVAFAGAQGNFELNAMRPIIINNTLHAIRVLGDACEKFRTYSVAGTELNRKRIDET
CCCCEEEEECCCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHH
VNQSLMLVTALSPVIGYDLASKIAHRANDEGTTLRAAALAEGVPAERFDAVCDPKTMIGH
HHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCHHHHHHHCCCHHHHCC
GLAGA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]