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The map label for this gene is poxB [H]

Identifier: 170751247

GI number: 170751247

Start: 5193372

End: 5195099

Strand: Reverse

Name: poxB [H]

Synonym: Mrad2831_4864

Alternate gene names: 170751247

Gene position: 5195099-5193372 (Counterclockwise)

Preceding gene: 170751248

Following gene: 170751246

Centisome position: 85.48

GC content: 72.34

Gene sequence:

>1728_bases
ATGAGCACGAAGGTCGCCGATCTGTTCGTCCGGGCGCTGGCGCAGGCCGGCGTGAAGCGCATCTACGGCGTGGTCGGCGA
CAGCCTCAACGGCCTGACCGAGGCCCTGCGCCAGGACGGCTCGATTGCCTGGATCCACACCCGCCACGAGGAGAGCGCCG
CCTTCGCGGCCGGCGCCGAGGCGCACCTGACCGGCGCGCTCGCGGTCTGCGCCGGTTCCTGCGGGCCCGGCAACCTGCAC
CTGATCAACGGCCTGTTCGACGCGCACCGCTCCCGCGTGCCCGTGCTGGCCATCGCGGCCCAGATCCCCTCGGCGGAGAT
CGGCCGCGCCTACTTCCAGGAGACGCACCCGCAGAACCTGTTCCAGGAATGCAGCCACTACTGCGAGTTGGTCTCCTCGC
CGGCCCAGCTGCCCGGCGTGATCGAGACCGCGATCCGCGTCGCCGTCGCCGAGCGCGGGGTCGCCGTGGTGGTGATCCCC
GGCGATGTCGGCCTCATGGCCATGCCGGGCGGGGCGCCCGACCTCTCGGCCGCCCTGGTGCCGCCCCCGCCCGTGGTGCG
CCCGCGCGACGCCGAGCTGGACGCCCTCGCCGGGATGCTCAACGCGGCCGGGCGCGTGACCCTGTTCTGCGGCCGCGGCT
GCGCCGGCGCCCACGCGGAGCTGCTGCAGCTCGCCGAGGCGCTGAAGGCCCCGGTCGTCCACGCGCTCGGCGGCAAGGAG
CACGTCGAGTGGGACAATCCCTACGACGTCGGCATGACCGGCCTGATCGGATTCTCGTCGGGCTACCGGGCGATGCTCGA
CTGCGACACCCTGCTGCTGCTCGGCACCGGCTTCCCCTACCGCCAGTTCTACCCGGAGGACGCCAAGGTCGCGCAGGTCG
ACCTGCGGGCCGGGAGCCTCGGGCGCCACTGCCGGATCGACCTCGGCCTGATCGGCGACGTCGGCGCGACCATCACGGCG
CTGCTGCCGCGGCTGCAGGCGAAGACCGACCGGGCGCATCTCGACGACAGCCTCGCCCATTACCGCAAGGCCCGCGCCGG
GCTCGACGCGCTGGCGGTCGGCAAGCCCGGCGGCAAGATCCACCCGCAATACGTCGCCCGGACGCTGAGCGAGGCGGCCT
CCGAGGACGCGGTCTTCACCTTCGACGTCGGCACGCCGACGATCTGGGCGGCGCGCTACCTCGCCATGAACGGCAAGCGC
CGGCTGATCGGCTCGCTCAACCACGGCTCCATGGCCAACGCCCTGCCGCAGGCGATCGGCGCGCAGGCGGCCTATCCCGG
GCGGCAGGTCATCTCGTTCTCGGGCGACGGCGGCTTCGCCATGCTGATGGGCGACCTGCTGACCCTCAACCAGCACAGGC
TGCCGGTGAAGATCGTGGTGCTGAACAACGGCACGCTGGGCTTCGTCGAGATGGAGATGAAGGCCGCGGGCTTCCTCGAG
ACCGGCGTCGCGCTGGTGAACCCGGACTTCGCCGCCATGGCGAGCGGGGCCGGCATCTTCGCCCGGCGCGTGGAGGATCC
GGCCGCGCTGCCGGACGCGATGCGCGCGATGCTGGACCACGACGGCCCGGCGCTCCTCGACGTCGTCTCGGCCCGCCAGG
AACTGTCGATGCCGCCGAAGATCGGCCTCGAGCAGGCGACGGGCTTCGGGATGTGGCTCGCCAAGGCGGTGATCGACGGG
CGCGGCACCGAGATCATCGACCTCGCCAAGACCAACCTCTGGCGGTGA

Upstream 100 bases:

>100_bases
CGACGCGGTGTGCGATCCCAAGACCATGATCGGCCACGGGCTGGCCGGCGCCTGACCCGCCGGGACCGTCCGTCCTGCCC
CCTCAGCTGAGCGAGACACC

Downstream 100 bases:

>100_bases
CGCCACCCGCGTCCCTCCCTCCGACCCGGAGTTCAGCCCGATGATCCGCTGCGTACGCATCTGGACCGGCGACGACGACA
ATTCCTACTTCGCGGAAGGC

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSTKVADLFVRALAQAGVKRIYGVVGDSLNGLTEALRQDGSIAWIHTRHEESAAFAAGAEAHLTGALAVCAGSCGPGNLH
LINGLFDAHRSRVPVLAIAAQIPSAEIGRAYFQETHPQNLFQECSHYCELVSSPAQLPGVIETAIRVAVAERGVAVVVIP
GDVGLMAMPGGAPDLSAALVPPPPVVRPRDAELDALAGMLNAAGRVTLFCGRGCAGAHAELLQLAEALKAPVVHALGGKE
HVEWDNPYDVGMTGLIGFSSGYRAMLDCDTLLLLGTGFPYRQFYPEDAKVAQVDLRAGSLGRHCRIDLGLIGDVGATITA
LLPRLQAKTDRAHLDDSLAHYRKARAGLDALAVGKPGGKIHPQYVARTLSEAASEDAVFTFDVGTPTIWAARYLAMNGKR
RLIGSLNHGSMANALPQAIGAQAAYPGRQVISFSGDGGFAMLMGDLLTLNQHRLPVKIVVLNNGTLGFVEMEMKAAGFLE
TGVALVNPDFAAMASGAGIFARRVEDPAALPDAMRAMLDHDGPALLDVVSARQELSMPPKIGLEQATGFGMWLAKAVIDG
RGTEIIDLAKTNLWR

Sequences:

>Translated_575_residues
MSTKVADLFVRALAQAGVKRIYGVVGDSLNGLTEALRQDGSIAWIHTRHEESAAFAAGAEAHLTGALAVCAGSCGPGNLH
LINGLFDAHRSRVPVLAIAAQIPSAEIGRAYFQETHPQNLFQECSHYCELVSSPAQLPGVIETAIRVAVAERGVAVVVIP
GDVGLMAMPGGAPDLSAALVPPPPVVRPRDAELDALAGMLNAAGRVTLFCGRGCAGAHAELLQLAEALKAPVVHALGGKE
HVEWDNPYDVGMTGLIGFSSGYRAMLDCDTLLLLGTGFPYRQFYPEDAKVAQVDLRAGSLGRHCRIDLGLIGDVGATITA
LLPRLQAKTDRAHLDDSLAHYRKARAGLDALAVGKPGGKIHPQYVARTLSEAASEDAVFTFDVGTPTIWAARYLAMNGKR
RLIGSLNHGSMANALPQAIGAQAAYPGRQVISFSGDGGFAMLMGDLLTLNQHRLPVKIVVLNNGTLGFVEMEMKAAGFLE
TGVALVNPDFAAMASGAGIFARRVEDPAALPDAMRAMLDHDGPALLDVVSARQELSMPPKIGLEQATGFGMWLAKAVIDG
RGTEIIDLAKTNLWR
>Mature_574_residues
STKVADLFVRALAQAGVKRIYGVVGDSLNGLTEALRQDGSIAWIHTRHEESAAFAAGAEAHLTGALAVCAGSCGPGNLHL
INGLFDAHRSRVPVLAIAAQIPSAEIGRAYFQETHPQNLFQECSHYCELVSSPAQLPGVIETAIRVAVAERGVAVVVIPG
DVGLMAMPGGAPDLSAALVPPPPVVRPRDAELDALAGMLNAAGRVTLFCGRGCAGAHAELLQLAEALKAPVVHALGGKEH
VEWDNPYDVGMTGLIGFSSGYRAMLDCDTLLLLGTGFPYRQFYPEDAKVAQVDLRAGSLGRHCRIDLGLIGDVGATITAL
LPRLQAKTDRAHLDDSLAHYRKARAGLDALAVGKPGGKIHPQYVARTLSEAASEDAVFTFDVGTPTIWAARYLAMNGKRR
LIGSLNHGSMANALPQAIGAQAAYPGRQVISFSGDGGFAMLMGDLLTLNQHRLPVKIVVLNNGTLGFVEMEMKAAGFLET
GVALVNPDFAAMASGAGIFARRVEDPAALPDAMRAMLDHDGPALLDVVSARQELSMPPKIGLEQATGFGMWLAKAVIDGR
GTEIIDLAKTNLWR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=26.4184397163121, Blast_Score=130, Evalue=5e-30,
Organism=Escherichia coli, GI1787096, Length=576, Percent_Identity=60.4166666666667, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=29.5081967213115, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=27.972027972028, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=26.4758497316637, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788716, Length=490, Percent_Identity=23.265306122449, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=28.141592920354, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=28.141592920354, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=554, Percent_Identity=24.5487364620939, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=21.1180124223602, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922626, Length=560, Percent_Identity=25.1785714285714, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 60535; Mature: 60404

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKVADLFVRALAQAGVKRIYGVVGDSLNGLTEALRQDGSIAWIHTRHEESAAFAAGAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCH
AHLTGALAVCAGSCGPGNLHLINGLFDAHRSRVPVLAIAAQIPSAEIGRAYFQETHPQNL
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHHHH
FQECSHYCELVSSPAQLPGVIETAIRVAVAERGVAVVVIPGDVGLMAMPGGAPDLSAALV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCC
PPPPVVRPRDAELDALAGMLNAAGRVTLFCGRGCAGAHAELLQLAEALKAPVVHALGGKE
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEECCCCCC
HVEWDNPYDVGMTGLIGFSSGYRAMLDCDTLLLLGTGFPYRQFYPEDAKVAQVDLRAGSL
CCCCCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCHHHCCCCCCCEEEEEECCCCC
GRHCRIDLGLIGDVGATITALLPRLQAKTDRAHLDDSLAHYRKARAGLDALAVGKPGGKI
CCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
HPQYVARTLSEAASEDAVFTFDVGTPTIWAARYLAMNGKRRLIGSLNHGSMANALPQAIG
CHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHC
AQAAYPGRQVISFSGDGGFAMLMGDLLTLNQHRLPVKIVVLNNGTLGFVEMEMKAAGFLE
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEHHHCCHHH
TGVALVNPDFAAMASGAGIFARRVEDPAALPDAMRAMLDHDGPALLDVVSARQELSMPPK
CCEEEECCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
IGLEQATGFGMWLAKAVIDGRGTEIIDLAKTNLWR
CCCHHCCCHHHHHHHHHHCCCCCEEEEEHHHCCCC
>Mature Secondary Structure 
STKVADLFVRALAQAGVKRIYGVVGDSLNGLTEALRQDGSIAWIHTRHEESAAFAAGAE
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCH
AHLTGALAVCAGSCGPGNLHLINGLFDAHRSRVPVLAIAAQIPSAEIGRAYFQETHPQNL
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHHHH
FQECSHYCELVSSPAQLPGVIETAIRVAVAERGVAVVVIPGDVGLMAMPGGAPDLSAALV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCC
PPPPVVRPRDAELDALAGMLNAAGRVTLFCGRGCAGAHAELLQLAEALKAPVVHALGGKE
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEECCCCCC
HVEWDNPYDVGMTGLIGFSSGYRAMLDCDTLLLLGTGFPYRQFYPEDAKVAQVDLRAGSL
CCCCCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCHHHCCCCCCCEEEEEECCCCC
GRHCRIDLGLIGDVGATITALLPRLQAKTDRAHLDDSLAHYRKARAGLDALAVGKPGGKI
CCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
HPQYVARTLSEAASEDAVFTFDVGTPTIWAARYLAMNGKRRLIGSLNHGSMANALPQAIG
CHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHC
AQAAYPGRQVISFSGDGGFAMLMGDLLTLNQHRLPVKIVVLNNGTLGFVEMEMKAAGFLE
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEHHHCCHHH
TGVALVNPDFAAMASGAGIFARRVEDPAALPDAMRAMLDHDGPALLDVVSARQELSMPPK
CCEEEECCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
IGLEQATGFGMWLAKAVIDGRGTEIIDLAKTNLWR
CCCHHCCCHHHHHHHHHHCCCCCEEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]