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The map label for this gene is lipA

Identifier: 170748594

GI number: 170748594

Start: 2317306

End: 2318289

Strand: Reverse

Name: lipA

Synonym: Mrad2831_2176

Alternate gene names: 170748594

Gene position: 2318289-2317306 (Counterclockwise)

Preceding gene: 170748595

Following gene: 170748593

Centisome position: 38.14

GC content: 69.21

Gene sequence:

>984_bases
ATGGCCGTCGTCCTCGACACCCTGAAGAACGATCCGCGGCCGGTCCGCCTGCGCCACCCCGAGAAGGCGCACCGGCCCGA
CCAGCCCGTTCAGGCCAAGAAGCCGGACTGGATCCGCGTGAAGGCACCCGGCTCCAGGGCCTGGTCCGAGACGCAGAAGA
TCGTGCGCGAGCACGGGCTGGTCACGGTCTGCGAGGAGGCGGGCTGCCCGAATATCGGCGAGTGCTGGGAGAAGCGGCAC
GCCACCTTCATGATCATGGGCGACACCTGCACGCGGGCCTGCGCCTTCTGCAACGTGCGCACAGGCCTGCCCGACGCCCT
CGACCTGGGCGAGCCGGACAAGATCGCCGATTCGGTCGCCAAGCTCGGCCTGCACCACGTGGTCATCACGTCGGTCGACC
GGGACGACCTGAGGGACGGCGGCGCCGAGCACTTCGCCCGCACCATCGCGGCGATCCGGCGGGCGAGCCCCGGCACGACG
GTCGAGATCCTGACCCCCGACTTCCTGCGCAAGGACGGGGCGCTCGAGGTCGTGGTCGCCGCCAAGCCCGACGTGTTCAA
CCACAATCTGGAGACCGTGCCGGCCAAGTACCTCACGGTTCGGCCGGGCGCGCGCTACTTCCACTCGGTGCGCCTGCTGC
AGCGGGTGAAGGAGCTCGACCCGGCGATCTTCACCAAGTCCGGCATCATGGTCGGGCTCGGCGAGGAGCGGAACGAGGTG
CTGCAGCTCATGGACGACCTGCGCTCGGCCGACGTCGACTTCCTGACCATCGGCCAGTACCTGCAGCCGTCGAAGAAGCA
CCACGAGGTCGTGCGCTTCGTCCCCCCCGACGAGTTCAAGGCCTACGAGACCACCGCCTACGCCAAGGGCTTCCTGCTGG
TCTCGGCCACGCCCCTGACGCGGTCGTCGCACCATGCGGGCGAGGACTTTGCGCGACTTCAGGCGGCGCGTCTGGCCAAA
CTGTCGCCCGCGCTCTCGGCCTGA

Upstream 100 bases:

>100_bases
CCCGGCGTCGCGGCGGTTTGCGGCGCGCGGCCTTGGGCCTTAAGTCAGGGCGATCCCGACCGCGCCCCTGGGGCGCTTGC
CCGCGGTGTGCCCCCGACCC

Downstream 100 bases:

>100_bases
GGAATCCCGAACACCATGCCGTCCTTCCGGGTCACCCGCGCGGTGAAGCACACGCCGCAGCAGATGTACGACCTCGTCGC
CGACGTGGAGCGCTATCCCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAVVLDTLKNDPRPVRLRHPEKAHRPDQPVQAKKPDWIRVKAPGSRAWSETQKIVREHGLVTVCEEAGCPNIGECWEKRH
ATFMIMGDTCTRACAFCNVRTGLPDALDLGEPDKIADSVAKLGLHHVVITSVDRDDLRDGGAEHFARTIAAIRRASPGTT
VEILTPDFLRKDGALEVVVAAKPDVFNHNLETVPAKYLTVRPGARYFHSVRLLQRVKELDPAIFTKSGIMVGLGEERNEV
LQLMDDLRSADVDFLTIGQYLQPSKKHHEVVRFVPPDEFKAYETTAYAKGFLLVSATPLTRSSHHAGEDFARLQAARLAK
LSPALSA

Sequences:

>Translated_327_residues
MAVVLDTLKNDPRPVRLRHPEKAHRPDQPVQAKKPDWIRVKAPGSRAWSETQKIVREHGLVTVCEEAGCPNIGECWEKRH
ATFMIMGDTCTRACAFCNVRTGLPDALDLGEPDKIADSVAKLGLHHVVITSVDRDDLRDGGAEHFARTIAAIRRASPGTT
VEILTPDFLRKDGALEVVVAAKPDVFNHNLETVPAKYLTVRPGARYFHSVRLLQRVKELDPAIFTKSGIMVGLGEERNEV
LQLMDDLRSADVDFLTIGQYLQPSKKHHEVVRFVPPDEFKAYETTAYAKGFLLVSATPLTRSSHHAGEDFARLQAARLAK
LSPALSA
>Mature_326_residues
AVVLDTLKNDPRPVRLRHPEKAHRPDQPVQAKKPDWIRVKAPGSRAWSETQKIVREHGLVTVCEEAGCPNIGECWEKRHA
TFMIMGDTCTRACAFCNVRTGLPDALDLGEPDKIADSVAKLGLHHVVITSVDRDDLRDGGAEHFARTIAAIRRASPGTTV
EILTPDFLRKDGALEVVVAAKPDVFNHNLETVPAKYLTVRPGARYFHSVRLLQRVKELDPAIFTKSGIMVGLGEERNEVL
QLMDDLRSADVDFLTIGQYLQPSKKHHEVVRFVPPDEFKAYETTAYAKGFLLVSATPLTRSSHHAGEDFARLQAARLAKL
SPALSA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=44.1379310344828, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=46.0905349794239, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=52.8985507246377, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=43.7984496124031, Blast_Score=227, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=298, Percent_Identity=43.2885906040268, Blast_Score=246, Evalue=4e-66,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=43.4628975265018, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_METRJ (B1LWN4)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754854.1
- ProteinModelPortal:   B1LWN4
- GeneID:   6138208
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_2176
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36245; Mature: 36114

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVLDTLKNDPRPVRLRHPEKAHRPDQPVQAKKPDWIRVKAPGSRAWSETQKIVREHGL
CEEEEECCCCCCCCEEECCCHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
VTVCEEAGCPNIGECWEKRHATFMIMGDTCTRACAFCNVRTGLPDALDLGEPDKIADSVA
EEEECCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
KLGLHHVVITSVDRDDLRDGGAEHFARTIAAIRRASPGTTVEILTPDFLRKDGALEVVVA
HHCHHEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEE
AKPDVFNHNLETVPAKYLTVRPGARYFHSVRLLQRVKELDPAIFTKSGIMVGLGEERNEV
CCCCHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHH
LQLMDDLRSADVDFLTIGQYLQPSKKHHEVVRFVPPDEFKAYETTAYAKGFLLVSATPLT
HHHHHHHHHCCCCEEECCHHHCCCCHHCCEEEECCCCCCCHHHHHHHHCEEEEEEECCCC
RSSHHAGEDFARLQAARLAKLSPALSA
CCCCCCCHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
AVVLDTLKNDPRPVRLRHPEKAHRPDQPVQAKKPDWIRVKAPGSRAWSETQKIVREHGL
EEEEECCCCCCCCEEECCCHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
VTVCEEAGCPNIGECWEKRHATFMIMGDTCTRACAFCNVRTGLPDALDLGEPDKIADSVA
EEEECCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
KLGLHHVVITSVDRDDLRDGGAEHFARTIAAIRRASPGTTVEILTPDFLRKDGALEVVVA
HHCHHEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEE
AKPDVFNHNLETVPAKYLTVRPGARYFHSVRLLQRVKELDPAIFTKSGIMVGLGEERNEV
CCCCHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHH
LQLMDDLRSADVDFLTIGQYLQPSKKHHEVVRFVPPDEFKAYETTAYAKGFLLVSATPLT
HHHHHHHHHCCCCEEECCHHHCCCCHHCCEEEECCCCCCCHHHHHHHHCEEEEEEECCCC
RSSHHAGEDFARLQAARLAKLSPALSA
CCCCCCCHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA