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The map label for this gene is glpF [H]

Identifier: 170748595

GI number: 170748595

Start: 2318424

End: 2319155

Strand: Reverse

Name: glpF [H]

Synonym: Mrad2831_2177

Alternate gene names: 170748595

Gene position: 2319155-2318424 (Counterclockwise)

Preceding gene: 170748596

Following gene: 170748594

Centisome position: 38.16

GC content: 70.22

Gene sequence:

>732_bases
ATGACATCACCCTTTCTGGGCGAATTCCTGGGCACGATGACCTTGATCGTCCTCGGCGACGGGGTCGTCGCCGGCGCCCT
GCTCAAGCATTCCAAGGGCGAGAATGCCGGCTGGATCGCCATCACGGCGGGCTGGGCCTTCGCGGTCCTCGCCGGTGTGT
TCGTGGCCAACGCCACCGGCAGCGCCGACGCCCACATCAACCCGGCCGTCACCGTGGCGGGCGCCGTCAGTTCCGGCGAT
TACAGCAAGCTCGCCACCTACATTCCGGCCCAGATGCTCGGCGCCTTCGTGGGCGCCGTGATCGTCTGGCTCCATTACCT
GCCGCATTGGGGTGCGACCCAGGATCCCGGCCTCAAGCTCGCCTGCTTCTGCACCGGCCCGGCGATCCGGGAGGCGAAGG
CCAACTGGCTGTCCGAGATCATCGCCACCTTCTTCCTGATCCTGACGATCTCCGCCCTCGTGACCACCACGCTGGGCTCG
GCCGGCGCGATCCCGCGGGCCGTCGCGCCCTACATGGTCGGCATGCTGGTCTGGGGCATCGGCCTGTCGCTCGGCGGGAC
CACCGGCTACGCCATCAACCCGGCCCGCGACCTGGGGCCGCGCGTGGCCCACGCGGTGCTGCCGATCGCAGGCAAGGGCA
GCTCCGACTGGGGCTACGCGCTGATCCCGGTGGCCGCGCCCCTGGTCGGGGCGGTGCTGGCCGGGCTGTTCGTCCGGGCG
CTCGGGATCTGA

Upstream 100 bases:

>100_bases
ATTCGGATCTGCGCAGATATCGGTGACCGCCACCGGCGGCCACACGCTCGCACAAGCTTTGAACGCTCACAAAAAAGCAG
CGAACGGGGGAGACGAAACG

Downstream 100 bases:

>100_bases
CGGAACGCCCGCACCCTCAACCGCCGCGGGGCGGCCCGGCGTCGCGGCGGTTTGCGGCGCGCGGCCTTGGGCCTTAAGTC
AGGGCGATCCCGACCGCGCC

Product: major intrinsic protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTSPFLGEFLGTMTLIVLGDGVVAGALLKHSKGENAGWIAITAGWAFAVLAGVFVANATGSADAHINPAVTVAGAVSSGD
YSKLATYIPAQMLGAFVGAVIVWLHYLPHWGATQDPGLKLACFCTGPAIREAKANWLSEIIATFFLILTISALVTTTLGS
AGAIPRAVAPYMVGMLVWGIGLSLGGTTGYAINPARDLGPRVAHAVLPIAGKGSSDWGYALIPVAAPLVGAVLAGLFVRA
LGI

Sequences:

>Translated_243_residues
MTSPFLGEFLGTMTLIVLGDGVVAGALLKHSKGENAGWIAITAGWAFAVLAGVFVANATGSADAHINPAVTVAGAVSSGD
YSKLATYIPAQMLGAFVGAVIVWLHYLPHWGATQDPGLKLACFCTGPAIREAKANWLSEIIATFFLILTISALVTTTLGS
AGAIPRAVAPYMVGMLVWGIGLSLGGTTGYAINPARDLGPRVAHAVLPIAGKGSSDWGYALIPVAAPLVGAVLAGLFVRA
LGI
>Mature_242_residues
TSPFLGEFLGTMTLIVLGDGVVAGALLKHSKGENAGWIAITAGWAFAVLAGVFVANATGSADAHINPAVTVAGAVSSGDY
SKLATYIPAQMLGAFVGAVIVWLHYLPHWGATQDPGLKLACFCTGPAIREAKANWLSEIIATFFLILTISALVTTTLGSA
GAIPRAVAPYMVGMLVWGIGLSLGGTTGYAINPARDLGPRVAHAVLPIAGKGSSDWGYALIPVAAPLVGAVLAGLFVRAL
GI

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=247, Percent_Identity=39.2712550607287, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4826645, Length=253, Percent_Identity=35.9683794466403, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI4502187, Length=251, Percent_Identity=33.8645418326693, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=34.5528455284553, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790362, Length=251, Percent_Identity=31.0756972111554, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994009, Length=237, Percent_Identity=31.2236286919831, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17533613, Length=246, Percent_Identity=29.6747967479675, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32564052, Length=246, Percent_Identity=29.6747967479675, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17544068, Length=244, Percent_Identity=30.7377049180328, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531431, Length=239, Percent_Identity=29.7071129707113, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17531429, Length=239, Percent_Identity=29.7071129707113, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71992966, Length=251, Percent_Identity=29.8804780876494, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=213, Percent_Identity=32.8638497652582, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322985, Length=294, Percent_Identity=26.1904761904762, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24695; Mature: 24564

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPFLGEFLGTMTLIVLGDGVVAGALLKHSKGENAGWIAITAGWAFAVLAGVFVANATG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCC
SADAHINPAVTVAGAVSSGDYSKLATYIPAQMLGAFVGAVIVWLHYLPHWGATQDPGLKL
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ACFCTGPAIREAKANWLSEIIATFFLILTISALVTTTLGSAGAIPRAVAPYMVGMLVWGI
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GLSLGGTTGYAINPARDLGPRVAHAVLPIAGKGSSDWGYALIPVAAPLVGAVLAGLFVRA
HHHCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
LGI
HCC
>Mature Secondary Structure 
TSPFLGEFLGTMTLIVLGDGVVAGALLKHSKGENAGWIAITAGWAFAVLAGVFVANATG
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCC
SADAHINPAVTVAGAVSSGDYSKLATYIPAQMLGAFVGAVIVWLHYLPHWGATQDPGLKL
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ACFCTGPAIREAKANWLSEIIATFFLILTISALVTTTLGSAGAIPRAVAPYMVGMLVWGI
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GLSLGGTTGYAINPARDLGPRVAHAVLPIAGKGSSDWGYALIPVAAPLVGAVLAGLFVRA
HHHCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
LGI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]