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The map label for this gene is yogA [H]

Identifier: 170746783

GI number: 170746783

Start: 368978

End: 369994

Strand: Reverse

Name: yogA [H]

Synonym: Mrad2831_0337

Alternate gene names: 170746783

Gene position: 369994-368978 (Counterclockwise)

Preceding gene: 170746784

Following gene: 170746782

Centisome position: 6.09

GC content: 73.25

Gene sequence:

>1017_bases
ATGAGCACCACGATGCGAGCCGTGATCGCCGTCGGGCAGGGCGGCCCCGAGGTCCTCCGGATCGTCGAGCGACCGCGCCC
CGAGCCGGGCGAGGGACAACTGCTCGTGCGGGTCGAGTCCGCGGGCATCAACCGGCCGGACGTGATGCAGCGCGAGGGCC
GCTACCCGCCGCCGCCCGGCGCCTCCGACGTGCTGGGGCTCGAACTCGCCGGCATCGTGGAGGCGCTCGGACCCGGTGCC
GGGCGCTTCCGGACCGGTGATCGGGTGATGGCGCTGGTCCATAGCGGCGCCTACGCCGAGTGGGCCGTCGTCGACGAGGC
GGTCGCGCTGAAGGTGCCGGACGGCCTGCCGGCGATCGAGGCGGGGGCGATCCCCGAGACCTACTTCACCGTCTGGAGCA
ACGTGTTCGAGCGTGCCCGGCTAAGGGCGGGCGAGAGCCTGCTGATCCACGGCGGCGCCTCGGGAATCGGCACCACCGCG
ATCCTGCTGGCGAAGGCCTGGGGCGCCCGCGTGATCGTGACCGCGGGTTCGTCCGAGAAATGCGCGGCCTGCCTCAAACT
CGGCGCGGACGCCGCGATCGACTATCGGCGGGAGGATTTCGTCGCCGCGGCCCGGGCCGCCACCGACGGGGGCGGGCCCG
ACGTGATCCTCGACATGGTCGGCGGCCCCTACATCGCCCGCAACCTCGACGCCGTGGCGGTCGACGGGCGCATCGCCCAG
ATCGCCTTCCAGCAGGGCTCGCGGGTGACGGAGATCGATCTCCAGCCGCTCCTGACGAAGCGGGTGACGCTGACGGGCTC
GACGCTGCGGGCTCGCCCGGTCGCCCTGAAGGCCCGCCTGGCCGAGGCCCTCGCGGAGCACGTCCTGCCGCTGCTCGCGG
CCGGGCGCGCGCGGCCGCCGATCGATTCCGTGTTCCCGTTCGACCGGGTGGCTGAAGCCCATGCCCGCATGGATGGGGGC
CAGCATGTCGGGAAGATCGTCCTGTCGATGGGTGACGCCGCGCGGGGAAGCCGCTGA

Upstream 100 bases:

>100_bases
CCCTCGCGGAAGCCTGGGCGGCGTTCGATCTGGCGCGGAGCCGGCAGGCCATGAAGGTGCTGCTTCTTCCCGAAGCGGCG
GGGAGCCAGGGGGCCGAGCG

Downstream 100 bases:

>100_bases
TGTCCGAGATGTCCGAGCGCGCCAACATGACCGGCGCCCACGCCCTGGCCGCGGCGCTCCACCGGCACGGGGTCCGCGAC
GTGTTCGGCCAGAGCATCCC

Product: PIG3 family NAD(P)H quinone oxidoreductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSTTMRAVIAVGQGGPEVLRIVERPRPEPGEGQLLVRVESAGINRPDVMQREGRYPPPPGASDVLGLELAGIVEALGPGA
GRFRTGDRVMALVHSGAYAEWAVVDEAVALKVPDGLPAIEAGAIPETYFTVWSNVFERARLRAGESLLIHGGASGIGTTA
ILLAKAWGARVIVTAGSSEKCAACLKLGADAAIDYRREDFVAAARAATDGGGPDVILDMVGGPYIARNLDAVAVDGRIAQ
IAFQQGSRVTEIDLQPLLTKRVTLTGSTLRARPVALKARLAEALAEHVLPLLAAGRARPPIDSVFPFDRVAEAHARMDGG
QHVGKIVLSMGDAARGSR

Sequences:

>Translated_338_residues
MSTTMRAVIAVGQGGPEVLRIVERPRPEPGEGQLLVRVESAGINRPDVMQREGRYPPPPGASDVLGLELAGIVEALGPGA
GRFRTGDRVMALVHSGAYAEWAVVDEAVALKVPDGLPAIEAGAIPETYFTVWSNVFERARLRAGESLLIHGGASGIGTTA
ILLAKAWGARVIVTAGSSEKCAACLKLGADAAIDYRREDFVAAARAATDGGGPDVILDMVGGPYIARNLDAVAVDGRIAQ
IAFQQGSRVTEIDLQPLLTKRVTLTGSTLRARPVALKARLAEALAEHVLPLLAAGRARPPIDSVFPFDRVAEAHARMDGG
QHVGKIVLSMGDAARGSR
>Mature_337_residues
STTMRAVIAVGQGGPEVLRIVERPRPEPGEGQLLVRVESAGINRPDVMQREGRYPPPPGASDVLGLELAGIVEALGPGAG
RFRTGDRVMALVHSGAYAEWAVVDEAVALKVPDGLPAIEAGAIPETYFTVWSNVFERARLRAGESLLIHGGASGIGTTAI
LLAKAWGARVIVTAGSSEKCAACLKLGADAAIDYRREDFVAAARAATDGGGPDVILDMVGGPYIARNLDAVAVDGRIAQI
AFQQGSRVTEIDLQPLLTKRVTLTGSTLRARPVALKARLAEALAEHVLPLLAAGRARPPIDSVFPFDRVAEAHARMDGGQ
HVGKIVLSMGDAARGSR

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=330, Percent_Identity=40.3030303030303, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI22538444, Length=330, Percent_Identity=40.3030303030303, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI194239674, Length=328, Percent_Identity=31.4024390243902, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI13236495, Length=328, Percent_Identity=31.4024390243902, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI24308257, Length=344, Percent_Identity=29.3604651162791, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI194239676, Length=327, Percent_Identity=29.3577981651376, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI18379349, Length=339, Percent_Identity=27.7286135693215, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI28557745, Length=323, Percent_Identity=29.7213622291022, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI41872631, Length=315, Percent_Identity=29.5238095238095, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI47519420, Length=385, Percent_Identity=26.7532467532467, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI197927207, Length=197, Percent_Identity=28.4263959390863, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1790485, Length=314, Percent_Identity=32.1656050955414, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787863, Length=350, Percent_Identity=26, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI226510941, Length=270, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=316, Percent_Identity=26.5822784810127, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI212642053, Length=311, Percent_Identity=29.2604501607717, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71987554, Length=203, Percent_Identity=31.5270935960591, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=354, Percent_Identity=26.271186440678, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324486, Length=354, Percent_Identity=26.271186440678, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319520, Length=328, Percent_Identity=26.219512195122, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323729, Length=354, Percent_Identity=23.728813559322, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319621, Length=352, Percent_Identity=24.7159090909091, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581345, Length=332, Percent_Identity=28.3132530120482, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920632, Length=310, Percent_Identity=29.0322580645161, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI221330659, Length=310, Percent_Identity=29.0322580645161, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550423, Length=170, Percent_Identity=28.8235294117647, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35377; Mature: 35246

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTMRAVIAVGQGGPEVLRIVERPRPEPGEGQLLVRVESAGINRPDVMQREGRYPPPPG
CCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCC
ASDVLGLELAGIVEALGPGAGRFRTGDRVMALVHSGAYAEWAVVDEAVALKVPDGLPAIE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCCC
AGAIPETYFTVWSNVFERARLRAGESLLIHGGASGIGTTAILLAKAWGARVIVTAGSSEK
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHH
CAACLKLGADAAIDYRREDFVAAARAATDGGGPDVILDMVGGPYIARNLDAVAVDGRIAQ
HHHHHHCCCCHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCCEEEECCEEEE
IAFQQGSRVTEIDLQPLLTKRVTLTGSTLRARPVALKARLAEALAEHVLPLLAAGRARPP
EHHHCCCEEEEECCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDSVFPFDRVAEAHARMDGGQHVGKIVLSMGDAARGSR
CHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STTMRAVIAVGQGGPEVLRIVERPRPEPGEGQLLVRVESAGINRPDVMQREGRYPPPPG
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCC
ASDVLGLELAGIVEALGPGAGRFRTGDRVMALVHSGAYAEWAVVDEAVALKVPDGLPAIE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCCC
AGAIPETYFTVWSNVFERARLRAGESLLIHGGASGIGTTAILLAKAWGARVIVTAGSSEK
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHH
CAACLKLGADAAIDYRREDFVAAARAATDGGGPDVILDMVGGPYIARNLDAVAVDGRIAQ
HHHHHHCCCCHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCCEEEECCEEEE
IAFQQGSRVTEIDLQPLLTKRVTLTGSTLRARPVALKARLAEALAEHVLPLLAAGRARPP
EHHHCCCEEEEECCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDSVFPFDRVAEAHARMDGGQHVGKIVLSMGDAARGSR
CHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]