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The map label for this gene is ilvB [H]

Identifier: 170746782

GI number: 170746782

Start: 367251

End: 368978

Strand: Reverse

Name: ilvB [H]

Synonym: Mrad2831_0336

Alternate gene names: 170746782

Gene position: 368978-367251 (Counterclockwise)

Preceding gene: 170746783

Following gene: 170746781

Centisome position: 6.07

GC content: 72.74

Gene sequence:

>1728_bases
ATGTCCGAGATGTCCGAGCGCGCCAACATGACCGGCGCCCACGCCCTGGCCGCGGCGCTCCACCGGCACGGGGTCCGCGA
CGTGTTCGGCCAGAGCATCCCGTCGGCGCTGTTCCTGGCCGCCCCGCATCACGGCATCCGCCAGATCGGCTACCGCACCG
AGAATGCCGGCGCCGCGATGGCCGACGCCTACGCGCGGATCTCGGGCCGGGTCGCGGTGGTCGCCGCCCAGAACGGTCCC
GCCGCCACCCTGCTGGTGCCGGGCCTCGCGGAAGCGCTGAAGGCGTCCGTACCCGTGGTGGCCATCGTCCAAGACGTCCA
CCGGCACTTCACGGACCGGAACGCCTTCCAGGAACTCGATCACCTCGCCCTGTTCGCGGGCGTCGCCAAGTGGGTCCGCC
GGGTCGCGGTCGCCGAGCGCATCGACGACTACGTCGACATGGCCTTCGCGGCCGCGGCCTCCGGGCGGCCCGGTCCGGCG
GTGCTGCTGGTGCCCCTGGACCTGCTCGACGAGCGGCCGGATTTCGAGGCCTCGGCACCGCGCCGAGCGGCCAGCCTCGG
CACCTTCCCCCTCGACCGCACGGTCGCCGACCCGGCCCGGATCGCGGAGGCCGCGGACCTGATCGCCGGGGCGCGGCGCC
CCCTCGTGATCGCGGGCGGCGGTGTCCATTCCTCCGGCGCCTATTCGGAACTCGGCGCGCTGCAGAGCCTCGGACTGCCG
GTCGCCACGACGGTGATGGGCAAGGGCGCCGTCGCCGAGACCGACCCGCTCACCGTCGGCGTCGTCGGCTACTTCATGGC
GCCGCGGGCGCGCTCGTCCCACCTCCGCACGCTCGTCACGGAGGCCGACGTCGTCCTCCTCGTGGGCAACCGCACCAATC
AGAACGGCACCGACAGCTGGTCGCTCTACCCGGCAGGCGCCCGATTCATCCATCTCGACGTGGACGGGGGCGAGGTCGGC
CGCAACTACGAGTCCGTTCGCCTCGTTGGCGACGCCAAGCTGACCCTCGCTTCCCTCGCCGAGGTCCTTCGGCAACGCGA
CCTGTCCGGTCTGAAAGGCCGCCGCGCGGCGCTCGCCGACACCATCGCCCGCGCCCGGGAGGCCCAGGCCGGCGACATGG
CCCGCCTCGTCGACATGGAGGCCACCCCGATCCGACCCGAGCGGATCATGGCCGAGATCGACGCGGTGACGACGCCCGAG
ACCGTCGTCGTCGCCGACGCGAGCTACGCCTCGATCTGGATCGCCAACTTCCTCACGGCGCGGAGGGCCGGCCAGCGCTT
CCTCACCCCGCGCGGCATCGCCGGACTCGGATGGGGCCTGCCCTTCGCCCTCGGCGCCAAGGTCGCTCGCCCGGACGCGC
CCGTGATCTGCGTGACCGGCGACGGCGGCTTCGGCCACGTCTGGTCGGAGCTGGAGACGGCGCGGCGCATGCGGCTGCCG
GTCATCGTCGTCGTCCTGAACAACCAGATCCTCGGCTACCAGAAGCACGCGGAGCTGAGCCTGTTCGGCGACTTCACCGA
CGTCTGCGACTTCGAGGCGGTGGACCATGCCGCGATCGCCCGCGCCTGCGGCTGCGCCGGGACCCGCGTCGAGCGCCCCG
GAGATCTCGCGCCGGCGCTGCGCGACGCACTCGCGAACGACGCCGTCACGGTGATCGACGTGATCACCGACCAGCGCGCC
TACCCGCCGATCACCAGCTTCGAGGGCAAGGACGCCCTCGCCTACTGA

Upstream 100 bases:

>100_bases
CGTTCGACCGGGTGGCTGAAGCCCATGCCCGCATGGATGGGGGCCAGCATGTCGGGAAGATCGTCCTGTCGATGGGTGAC
GCCGCGCGGGGAAGCCGCTG

Downstream 100 bases:

>100_bases
AGCCCCCGACTGGCCCCAACGAGAAGGCGCACGAAGCGCCGGGCCGACGACTGACACGGGAGGAAACGATGAGAGTTCTG
GCGCGTGCACTCGCCGCCGC

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSEMSERANMTGAHALAAALHRHGVRDVFGQSIPSALFLAAPHHGIRQIGYRTENAGAAMADAYARISGRVAVVAAQNGP
AATLLVPGLAEALKASVPVVAIVQDVHRHFTDRNAFQELDHLALFAGVAKWVRRVAVAERIDDYVDMAFAAAASGRPGPA
VLLVPLDLLDERPDFEASAPRRAASLGTFPLDRTVADPARIAEAADLIAGARRPLVIAGGGVHSSGAYSELGALQSLGLP
VATTVMGKGAVAETDPLTVGVVGYFMAPRARSSHLRTLVTEADVVLLVGNRTNQNGTDSWSLYPAGARFIHLDVDGGEVG
RNYESVRLVGDAKLTLASLAEVLRQRDLSGLKGRRAALADTIARAREAQAGDMARLVDMEATPIRPERIMAEIDAVTTPE
TVVVADASYASIWIANFLTARRAGQRFLTPRGIAGLGWGLPFALGAKVARPDAPVICVTGDGGFGHVWSELETARRMRLP
VIVVVLNNQILGYQKHAELSLFGDFTDVCDFEAVDHAAIARACGCAGTRVERPGDLAPALRDALANDAVTVIDVITDQRA
YPPITSFEGKDALAY

Sequences:

>Translated_575_residues
MSEMSERANMTGAHALAAALHRHGVRDVFGQSIPSALFLAAPHHGIRQIGYRTENAGAAMADAYARISGRVAVVAAQNGP
AATLLVPGLAEALKASVPVVAIVQDVHRHFTDRNAFQELDHLALFAGVAKWVRRVAVAERIDDYVDMAFAAAASGRPGPA
VLLVPLDLLDERPDFEASAPRRAASLGTFPLDRTVADPARIAEAADLIAGARRPLVIAGGGVHSSGAYSELGALQSLGLP
VATTVMGKGAVAETDPLTVGVVGYFMAPRARSSHLRTLVTEADVVLLVGNRTNQNGTDSWSLYPAGARFIHLDVDGGEVG
RNYESVRLVGDAKLTLASLAEVLRQRDLSGLKGRRAALADTIARAREAQAGDMARLVDMEATPIRPERIMAEIDAVTTPE
TVVVADASYASIWIANFLTARRAGQRFLTPRGIAGLGWGLPFALGAKVARPDAPVICVTGDGGFGHVWSELETARRMRLP
VIVVVLNNQILGYQKHAELSLFGDFTDVCDFEAVDHAAIARACGCAGTRVERPGDLAPALRDALANDAVTVIDVITDQRA
YPPITSFEGKDALAY
>Mature_574_residues
SEMSERANMTGAHALAAALHRHGVRDVFGQSIPSALFLAAPHHGIRQIGYRTENAGAAMADAYARISGRVAVVAAQNGPA
ATLLVPGLAEALKASVPVVAIVQDVHRHFTDRNAFQELDHLALFAGVAKWVRRVAVAERIDDYVDMAFAAAASGRPGPAV
LLVPLDLLDERPDFEASAPRRAASLGTFPLDRTVADPARIAEAADLIAGARRPLVIAGGGVHSSGAYSELGALQSLGLPV
ATTVMGKGAVAETDPLTVGVVGYFMAPRARSSHLRTLVTEADVVLLVGNRTNQNGTDSWSLYPAGARFIHLDVDGGEVGR
NYESVRLVGDAKLTLASLAEVLRQRDLSGLKGRRAALADTIARAREAQAGDMARLVDMEATPIRPERIMAEIDAVTTPET
VVVADASYASIWIANFLTARRAGQRFLTPRGIAGLGWGLPFALGAKVARPDAPVICVTGDGGFGHVWSELETARRMRLPV
IVVVLNNQILGYQKHAELSLFGDFTDVCDFEAVDHAAIARACGCAGTRVERPGDLAPALRDALANDAVTVIDVITDQRAY
PPITSFEGKDALAY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=592, Percent_Identity=25.8445945945946, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI21361361, Length=518, Percent_Identity=28.3783783783784, Blast_Score=142, Evalue=8e-34,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=29.0780141843972, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=28.8530465949821, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1790104, Length=505, Percent_Identity=27.7227722772277, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787096, Length=510, Percent_Identity=26.2745098039216, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788716, Length=578, Percent_Identity=24.7404844290657, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=591, Percent_Identity=29.4416243654822, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=591, Percent_Identity=29.4416243654822, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=568, Percent_Identity=23.2394366197183, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=25.9130434782609, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=573, Percent_Identity=24.78184991274, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922626, Length=530, Percent_Identity=27.9245283018868, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60820; Mature: 60689

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMSERANMTGAHALAAALHRHGVRDVFGQSIPSALFLAAPHHGIRQIGYRTENAGAAM
CCCHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCCCHHH
ADAYARISGRVAVVAAQNGPAATLLVPGLAEALKASVPVVAIVQDVHRHFTDRNAFQELD
HHHHHHHCCCEEEEEECCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHH
HLALFAGVAKWVRRVAVAERIDDYVDMAFAAAASGRPGPAVLLVPLDLLDERPDFEASAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCC
RRAASLGTFPLDRTVADPARIAEAADLIAGARRPLVIAGGGVHSSGAYSELGALQSLGLP
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCC
VATTVMGKGAVAETDPLTVGVVGYFMAPRARSSHLRTLVTEADVVLLVGNRTNQNGTDSW
HHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCE
SLYPAGARFIHLDVDGGEVGRNYESVRLVGDAKLTLASLAEVLRQRDLSGLKGRRAALAD
EEEECCCEEEEEECCCCHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TIARAREAQAGDMARLVDMEATPIRPERIMAEIDAVTTPETVVVADASYASIWIANFLTA
HHHHHHHCCCCCHHHHEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
RRAGQRFLTPRGIAGLGWGLPFALGAKVARPDAPVICVTGDGGFGHVWSELETARRMRLP
HHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
VIVVVLNNQILGYQKHAELSLFGDFTDVCDFEAVDHAAIARACGCAGTRVERPGDLAPAL
EEEEEECCCEECCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RDALANDAVTVIDVITDQRAYPPITSFEGKDALAY
HHHHCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEMSERANMTGAHALAAALHRHGVRDVFGQSIPSALFLAAPHHGIRQIGYRTENAGAAM
CCHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCCCHHH
ADAYARISGRVAVVAAQNGPAATLLVPGLAEALKASVPVVAIVQDVHRHFTDRNAFQELD
HHHHHHHCCCEEEEEECCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHH
HLALFAGVAKWVRRVAVAERIDDYVDMAFAAAASGRPGPAVLLVPLDLLDERPDFEASAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCC
RRAASLGTFPLDRTVADPARIAEAADLIAGARRPLVIAGGGVHSSGAYSELGALQSLGLP
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCC
VATTVMGKGAVAETDPLTVGVVGYFMAPRARSSHLRTLVTEADVVLLVGNRTNQNGTDSW
HHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCE
SLYPAGARFIHLDVDGGEVGRNYESVRLVGDAKLTLASLAEVLRQRDLSGLKGRRAALAD
EEEECCCEEEEEECCCCHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TIARAREAQAGDMARLVDMEATPIRPERIMAEIDAVTTPETVVVADASYASIWIANFLTA
HHHHHHHCCCCCHHHHEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
RRAGQRFLTPRGIAGLGWGLPFALGAKVARPDAPVICVTGDGGFGHVWSELETARRMRLP
HHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
VIVVVLNNQILGYQKHAELSLFGDFTDVCDFEAVDHAAIARACGCAGTRVERPGDLAPAL
EEEEEECCCEECCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RDALANDAVTVIDVITDQRAYPPITSFEGKDALAY
HHHHCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9099862 [H]