Click here to switch to the map view.

The map label for this gene is gutB [H]

Identifier: 170746784

GI number: 170746784

Start: 369991

End: 371049

Strand: Reverse

Name: gutB [H]

Synonym: Mrad2831_0338

Alternate gene names: 170746784

Gene position: 371049-369991 (Counterclockwise)

Preceding gene: 170746788

Following gene: 170746783

Centisome position: 6.1

GC content: 73.94

Gene sequence:

>1059_bases
ATGCTCGCGTTGCGCAAGGCGACGGCCGACTTCGGGGCTGACCTCGTCCACGTCGATGCGCCCACCGCTCCGGGGGCTGG
CCGCGTGACCGTCACGGTCGCCGCGGCCGGCATCTGCGGCAGCGACATCCACGCCTACGAGTGGACCCCCGGCTACGGGT
TCATGATCCCCTTCATGCCGGTCACCCTCGGCCACGAGTTCGCCGGGACGGTCCGCGCCGTGGGAGACGGCGTGACCGGG
CTCGCCGTCGGCGACCGGGTCACCTGCTGGCCGACAGTGCCGTGCGGCGGCTGCGCGGCCTGCGCGGCCGAGCGGCCGCA
GGATTGCCAGGGAAGGCGAGTCGTCGGCCTGCACGCGGACGGGGCCTTCGCCGAGCAGGTCGTCGTTCCGGCCGATAATT
GCCGCCGCCTGCCCGACGGGATGCCGCTGGCCGTCGCGGCGCTGGCCGAGCCGCTGGCGGTCGCGGTCAACGCGGTGGAC
GTGGCGGAGGTCGGGACGGGCGACCGCGTGGCGGTGCTCGGCCCGGGCCCGATCGGCCTCAGCATCGCCTTCGTGGCGCA
GCATCGGGGCGCGGACGTGCTGCTCGCCGGTCTCGACGACACCGCGCGCCTCGACCTCGCTCGCCGGATCGGCATCCGCG
CGACGGCCGATCTCGCCGGCGTCGGCCTGGACGACGCCGTTCGGCGCACCTTCGACGGGGAGGCTGACCGGGTGATCGAG
GCGACGGGATCCGCCCGATCGGTGGTCGACGGGATGGGCGTGCTGCGTTCCGGCGGCGTGATGGTGGTGGCAGGCATCCA
CTCCGGATCCCTCGACCTCGACCTGACGGGTTTCGTGCGCAGCAAGAAGCAGCTGCGCGCGGCCCACGACACGACGGCGC
GCGCCTTCGACGCCGCGATCGCGCTCCTCGCGGACCATGCCGGGACGCTGGAGGCGCTGATCACGCACCGTCGTCCCCTC
GCGGAAGCCTGGGCGGCGTTCGATCTGGCGCGGAGCCGGCAGGCCATGAAGGTGCTGCTTCTTCCCGAAGCGGCGGGGAG
CCAGGGGGCCGAGCGATGA

Upstream 100 bases:

>100_bases
GGTCCGATGTTTGTTATAACAAATTACGAAGTGCGTCGACGGCACGCGCCGTTGGGCATGCCGCCGGGCCGAGAGGCTCG
GCGGGAGACAGGGGACGGAA

Downstream 100 bases:

>100_bases
GCACCACGATGCGAGCCGTGATCGCCGTCGGGCAGGGCGGCCCCGAGGTCCTCCGGATCGTCGAGCGACCGCGCCCCGAG
CCGGGCGAGGGACAACTGCT

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MLALRKATADFGADLVHVDAPTAPGAGRVTVTVAAAGICGSDIHAYEWTPGYGFMIPFMPVTLGHEFAGTVRAVGDGVTG
LAVGDRVTCWPTVPCGGCAACAAERPQDCQGRRVVGLHADGAFAEQVVVPADNCRRLPDGMPLAVAALAEPLAVAVNAVD
VAEVGTGDRVAVLGPGPIGLSIAFVAQHRGADVLLAGLDDTARLDLARRIGIRATADLAGVGLDDAVRRTFDGEADRVIE
ATGSARSVVDGMGVLRSGGVMVVAGIHSGSLDLDLTGFVRSKKQLRAAHDTTARAFDAAIALLADHAGTLEALITHRRPL
AEAWAAFDLARSRQAMKVLLLPEAAGSQGAER

Sequences:

>Translated_352_residues
MLALRKATADFGADLVHVDAPTAPGAGRVTVTVAAAGICGSDIHAYEWTPGYGFMIPFMPVTLGHEFAGTVRAVGDGVTG
LAVGDRVTCWPTVPCGGCAACAAERPQDCQGRRVVGLHADGAFAEQVVVPADNCRRLPDGMPLAVAALAEPLAVAVNAVD
VAEVGTGDRVAVLGPGPIGLSIAFVAQHRGADVLLAGLDDTARLDLARRIGIRATADLAGVGLDDAVRRTFDGEADRVIE
ATGSARSVVDGMGVLRSGGVMVVAGIHSGSLDLDLTGFVRSKKQLRAAHDTTARAFDAAIALLADHAGTLEALITHRRPL
AEAWAAFDLARSRQAMKVLLLPEAAGSQGAER
>Mature_352_residues
MLALRKATADFGADLVHVDAPTAPGAGRVTVTVAAAGICGSDIHAYEWTPGYGFMIPFMPVTLGHEFAGTVRAVGDGVTG
LAVGDRVTCWPTVPCGGCAACAAERPQDCQGRRVVGLHADGAFAEQVVVPADNCRRLPDGMPLAVAALAEPLAVAVNAVD
VAEVGTGDRVAVLGPGPIGLSIAFVAQHRGADVLLAGLDDTARLDLARRIGIRATADLAGVGLDDAVRRTFDGEADRVIE
ATGSARSVVDGMGVLRSGGVMVVAGIHSGSLDLDLTGFVRSKKQLRAAHDTTARAFDAAIALLADHAGTLEALITHRRPL
AEAWAAFDLARSRQAMKVLLLPEAAGSQGAER

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=332, Percent_Identity=29.8192771084337, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI4501929, Length=355, Percent_Identity=25.0704225352113, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4501933, Length=347, Percent_Identity=25.3602305475504, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI34577061, Length=347, Percent_Identity=24.7838616714697, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI156523966, Length=370, Percent_Identity=25.4054054054054, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=25.4847645429363, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI226510992, Length=320, Percent_Identity=32.5, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790045, Length=341, Percent_Identity=30.2052785923754, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787863, Length=338, Percent_Identity=31.0650887573964, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788407, Length=334, Percent_Identity=28.1437125748503, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790718, Length=317, Percent_Identity=29.3375394321767, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788073, Length=311, Percent_Identity=29.5819935691318, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1788075, Length=360, Percent_Identity=29.1666666666667, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082125, Length=336, Percent_Identity=26.4880952380952, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786825, Length=419, Percent_Identity=25.5369928400955, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081918, Length=265, Percent_Identity=29.4339622641509, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=353, Percent_Identity=33.9943342776204, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17562878, Length=344, Percent_Identity=32.8488372093023, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25146526, Length=360, Percent_Identity=24.4444444444444, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71997431, Length=377, Percent_Identity=24.1379310344828, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565904, Length=319, Percent_Identity=24.7648902821317, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323099, Length=328, Percent_Identity=29.5731707317073, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322619, Length=335, Percent_Identity=30.1492537313433, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319955, Length=335, Percent_Identity=29.8507462686567, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319257, Length=273, Percent_Identity=31.5018315018315, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319258, Length=233, Percent_Identity=32.618025751073, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320033, Length=360, Percent_Identity=24.7222222222222, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=265, Percent_Identity=27.5471698113208, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17137530, Length=310, Percent_Identity=33.2258064516129, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI17737897, Length=320, Percent_Identity=33.125, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI221457811, Length=308, Percent_Identity=28.2467532467532, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45550770, Length=308, Percent_Identity=28.2467532467532, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45551930, Length=308, Percent_Identity=28.2467532467532, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17737895, Length=353, Percent_Identity=22.9461756373938, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36114; Mature: 36114

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALRKATADFGADLVHVDAPTAPGAGRVTVTVAAAGICGSDIHAYEWTPGYGFMIPFMP
CCEEECCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCEEECCEE
VTLGHEFAGTVRAVGDGVTGLAVGDRVTCWPTVPCGGCAACAAERPQDCQGRRVVGLHAD
EECCCHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCCCCCCEEEEEECC
GAFAEQVVVPADNCRRLPDGMPLAVAALAEPLAVAVNAVDVAEVGTGDRVAVLGPGPIGL
CCCCCEEEECCHHHHCCCCCCCHHHHHHHCCHHHHEEEEEHEECCCCCEEEEECCCCCCE
SIAFVAQHRGADVLLAGLDDTARLDLARRIGIRATADLAGVGLDDAVRRTFDGEADRVIE
EEEEEEECCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCCCEEE
ATGSARSVVDGMGVLRSGGVMVVAGIHSGSLDLDLTGFVRSKKQLRAAHDTTARAFDAAI
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ALLADHAGTLEALITHRRPLAEAWAAFDLARSRQAMKVLLLPEAAGSQGAER
HHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MLALRKATADFGADLVHVDAPTAPGAGRVTVTVAAAGICGSDIHAYEWTPGYGFMIPFMP
CCEEECCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCEEECCEE
VTLGHEFAGTVRAVGDGVTGLAVGDRVTCWPTVPCGGCAACAAERPQDCQGRRVVGLHAD
EECCCHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCCCCCCEEEEEECC
GAFAEQVVVPADNCRRLPDGMPLAVAALAEPLAVAVNAVDVAEVGTGDRVAVLGPGPIGL
CCCCCEEEECCHHHHCCCCCCCHHHHHHHCCHHHHEEEEEHEECCCCCEEEEECCCCCCE
SIAFVAQHRGADVLLAGLDDTARLDLARRIGIRATADLAGVGLDDAVRRTFDGEADRVIE
EEEEEEECCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCCCEEE
ATGSARSVVDGMGVLRSGGVMVVAGIHSGSLDLDLTGFVRSKKQLRAAHDTTARAFDAAI
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ALLADHAGTLEALITHRRPLAEAWAAFDLARSRQAMKVLLLPEAAGSQGAER
HHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]