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The map label for this gene is hemK [H]

Identifier: 170761878

GI number: 170761878

Start: 3040

End: 3912

Strand: Direct

Name: hemK [H]

Synonym: UPA3_0004

Alternate gene names: 170761878

Gene position: 3040-3912 (Clockwise)

Preceding gene: 170762199

Following gene: 170762116

Centisome position: 0.4

GC content: 22.45

Gene sequence:

>873_bases
ATGACCTACCATCAATTAATTTTTCAAGCATACTCACTACTTGAAAAAAAATTACGTAATCCACAAGTAGCATTTCAGTT
GCTGTACGGATTAGATAATAAAATTAACGATTCTTATAGTTTTTCAAATAACCGCTTAACAATTGTAAATTCTAATTTAG
AATATACATATTTTAAACTATTAGATGAATTTATTAATGGAAAACCATTAGTGAGAATTTTAGGATATGGCTATTTTTGT
GCAAAGCGCTTTTATGTTGATAAAAATGTTTTTGCTTTTCGTGTTGAAACTGAATTATTAATAGATGTTGTTAATAAAGT
AATTCAACAATCCACTTATAAAATTAAAAATGTTATTGATGTTTGTTGTGGTAGTGGTGTTTTAGGGTTAAGTACAAAAA
TGAATTTTAATCAATTGAATGTTTCATTATTAGATATTTCTATTGATGCAATTAATAATTGCAAAAAAAATGCTAAATAT
CATAACTTAACAGATGTAAATTTTATTCATAAAAGTATGCAAGAATACTTTTTAAACACAAAAAAACGGTTTGATTTAAT
TATCTGTAATCCCCCATATATCAAAAGTAATTATGAATTAAATCGTGAAGTCTTAGATTATGACCCCATTAATGCACTTA
TTGATTTCGATCACAAAGATGGAATTAGTTTTTATTTATTTATAATAAATAACATTAAATCAATTGCTAATGAGAAATTC
ACTATTATTTTTGAAATTGGTTTTGATCAAAAAGAAATTTTAGAAAAAGTTATTAAAAAAAATGAATTTCCTCTTTTTTA
CTACTTTATAAATGATTATAATAATCTATGTAGAATTTTAATAATAAGTAATATTAAATATGAAAATTTATAG

Upstream 100 bases:

>100_bases
TAAATAAATTAGACCAAATCATGATGGGTGAGTTAGATGAGATTATTGATGCATTAATTGCTGACGAACAAACTGGTTTA
ATGGCTAATTTAGATATTTA

Downstream 100 bases:

>100_bases
AATAACTAATTTAAATGTGATTTATGATGCTTTAATGGCCAATAAATGTGTTCTAATCCCTACTGATACTATCATTGGTT
TATTAGCAAAAAATCAAGAT

Product: HemK family methyltransferase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTYHQLIFQAYSLLEKKLRNPQVAFQLLYGLDNKINDSYSFSNNRLTIVNSNLEYTYFKLLDEFINGKPLVRILGYGYFC
AKRFYVDKNVFAFRVETELLIDVVNKVIQQSTYKIKNVIDVCCGSGVLGLSTKMNFNQLNVSLLDISIDAINNCKKNAKY
HNLTDVNFIHKSMQEYFLNTKKRFDLIICNPPYIKSNYELNREVLDYDPINALIDFDHKDGISFYLFIINNIKSIANEKF
TIIFEIGFDQKEILEKVIKKNEFPLFYYFINDYNNLCRILIISNIKYENL

Sequences:

>Translated_290_residues
MTYHQLIFQAYSLLEKKLRNPQVAFQLLYGLDNKINDSYSFSNNRLTIVNSNLEYTYFKLLDEFINGKPLVRILGYGYFC
AKRFYVDKNVFAFRVETELLIDVVNKVIQQSTYKIKNVIDVCCGSGVLGLSTKMNFNQLNVSLLDISIDAINNCKKNAKY
HNLTDVNFIHKSMQEYFLNTKKRFDLIICNPPYIKSNYELNREVLDYDPINALIDFDHKDGISFYLFIINNIKSIANEKF
TIIFEIGFDQKEILEKVIKKNEFPLFYYFINDYNNLCRILIISNIKYENL
>Mature_289_residues
TYHQLIFQAYSLLEKKLRNPQVAFQLLYGLDNKINDSYSFSNNRLTIVNSNLEYTYFKLLDEFINGKPLVRILGYGYFCA
KRFYVDKNVFAFRVETELLIDVVNKVIQQSTYKIKNVIDVCCGSGVLGLSTKMNFNQLNVSLLDISIDAINNCKKNAKYH
NLTDVNFIHKSMQEYFLNTKKRFDLIICNPPYIKSNYELNREVLDYDPINALIDFDHKDGISFYLFIINNIKSIANEKFT
IIFEIGFDQKEILEKVIKKNEFPLFYYFINDYNNLCRILIISNIKYENL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI87082085, Length=191, Percent_Identity=25.6544502617801, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR022438
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 34168; Mature: 34037

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00092 N6_MTASE ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYHQLIFQAYSLLEKKLRNPQVAFQLLYGLDNKINDSYSFSNNRLTIVNSNLEYTYFKL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCHHHHHH
LDEFINGKPLVRILGYGYFCAKRFYVDKNVFAFRVETELLIDVVNKVIQQSTYKIKNVID
HHHHHCCCCEEHHHHCHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VCCGSGVLGLSTKMNFNQLNVSLLDISIDAINNCKKNAKYHNLTDVNFIHKSMQEYFLNT
HHCCCCEEECEEECCCCEEEEEEEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
KKRFDLIICNPPYIKSNYELNREVLDYDPINALIDFDHKDGISFYLFIINNIKSIANEKF
CCCEEEEEECCCCCCCCCCCCCEECCCCCCCCEEECCCCCCCEEEEEEHHHHHHHCCCCE
TIIFEIGFDQKEILEKVIKKNEFPLFYYFINDYNNLCRILIISNIKYENL
EEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
TYHQLIFQAYSLLEKKLRNPQVAFQLLYGLDNKINDSYSFSNNRLTIVNSNLEYTYFKL
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCHHHHHH
LDEFINGKPLVRILGYGYFCAKRFYVDKNVFAFRVETELLIDVVNKVIQQSTYKIKNVID
HHHHHCCCCEEHHHHCHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VCCGSGVLGLSTKMNFNQLNVSLLDISIDAINNCKKNAKYHNLTDVNFIHKSMQEYFLNT
HHCCCCEEECEEECCCCEEEEEEEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
KKRFDLIICNPPYIKSNYELNREVLDYDPINALIDFDHKDGISFYLFIINNIKSIANEKF
CCCEEEEEECCCCCCCCCCCCCEECCCCCCCCEEECCCCCCCEEEEEEHHHHHHHCCCCE
TIIFEIGFDQKEILEKVIKKNEFPLFYYFINDYNNLCRILIISNIKYENL
EEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA