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The map label for this gene is prfA

Identifier: 170762199

GI number: 170762199

Start: 1961

End: 3040

Strand: Direct

Name: prfA

Synonym: UPA3_0003

Alternate gene names: 170762199

Gene position: 1961-3040 (Clockwise)

Preceding gene: 170762277

Following gene: 170761878

Centisome position: 0.26

GC content: 32.04

Gene sequence:

>1080_bases
ATGGAATACAACAAAAAACTTTATGAAGCAATTGAACGAGTCGCTATTAAAAATGACGCTTTAAAAAAAGAATTAGAAAC
GGTTGTAACAGACTTTAAAAAAATTAAAGAGATTAATATACAGTTAAAAAAAACTACTAAAATTGCTGAAGCCTTTGCAA
AATATAAACAAAAATTGGATACTGGTATAGCTGCTGAAAAAATATTAAATACAGAAAAAGATTTAGAATTAATCGAATTA
GCACAGATGGATTTAGATGAAGCTAAAATTAATATTCCAATAATTGAAAATGATTTAAAAATTATGCTGTTGCCAACTGA
TCCTAATGATGATAAAAATGTTATTGTTGAAATGCGCCCTGCTGCTGGAGGGGATGAATCATCAATTTTTGTCGGCAATT
TGTTTGATACATATCGTGCTTATGCTGAAAATAATAATTGAAAAATGAAAATTATAGAAATGACTCCGAATGCTGTTGGT
TTTAGTTTTATTTCTTTTATGATTTCTGGAGAAGAAGTTTATTCACGAATGAAATTTGAGTCAGGAGTTCATCGAGTACA
ACGTGTACCTGCTACTGAATCTAAAGGAAGAGTACATACGTCAACAATTACAGTTGCTGTTCTTCCAGAACAAGATGAAG
TTGATGTTGTAATTAATCCAACAGAATTAAGAATTGATACTTACCGTGCAAGTGGGGCTGGAGGACAGCATGTTAATAGA
ACAGAATCAGCTGTAAGAATTACGCATATTCCAACAGGGGTTGTTGCTGCATGTCAAGAGGGAAAATCACAAATTGAAAA
CCGAGAAACAGCTATGAAAATGTTAAGAGCTAAATTATGAGAAGCAGCTCAAGAACAGCAAAATGCAGAATTTGCTAATT
TAAGAAAAAATCAAGTTGGTACAGGGGATCGTTCTGAAAAAATCCGTACTTACAATTATCCACAGAACCGCGTTACTGAC
CATCGTATTAACCTAACATTAAATAAATTAGACCAAATCATGATGGGTGAGTTAGATGAGATTATTGATGCATTAATTGC
TGACGAACAAACTGGTTTAATGGCTAATTTAGATATTTAA

Upstream 100 bases:

>100_bases
TTAATTCTTTTTGTATAATTAAAAATTAGTAGATTAAATAACAGGCAATTAGATTACCTGTTATTTTATTTTTACTAAAA
TATTTAAAACTGAGGGACTT

Downstream 100 bases:

>100_bases
TGACCTACCATCAATTAATTTTTCAAGCATACTCACTACTTGAAAAAAAATTACGTAATCCACAAGTAGCATTTCAGTTG
CTGTACGGATTAGATAATAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLDTGIAAEKILNTEKDLELIEL
AQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRPAAGGDESSIFVGNLFDTYRAYAENNNWKMKIIEMTPNAVG
FSFISFMISGEEVYSRMKFESGVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNR
TESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKLWEAAQEQQNAEFANLRKNQVGTGDRSEKIRTYNYPQNRVTD
HRINLTLNKLDQIMMGELDEIIDALIADEQTGLMANLDI

Sequences:

>Translated_359_residues
MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLDTGIAAEKILNTEKDLELIEL
AQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRPAAGGDESSIFVGNLFDTYRAYAENNN*KMKIIEMTPNAVG
FSFISFMISGEEVYSRMKFESGVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNR
TESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKL*EAAQEQQNAEFANLRKNQVGTGDRSEKIRTYNYPQNRVTD
HRINLTLNKLDQIMMGELDEIIDALIADEQTGLMANLDI
>Mature_359_residues
MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLDTGIAAEKILNTEKDLELIEL
AQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRPAAGGDESSIFVGNLFDTYRAYAENNN*KMKIIEMTPNAVG
FSFISFMISGEEVYSRMKFESGVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNR
TESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKL*EAAQEQQNAEFANLRKNQVGTGDRSEKIRTYNYPQNRVTD
HRINLTLNKLDQIMMGELDEIIDALIADEQTGLMANLDI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=283, Percent_Identity=48.4098939929329, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI34577120, Length=380, Percent_Identity=35.2631578947368, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI166795305, Length=188, Percent_Identity=45.7446808510638, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1787462, Length=333, Percent_Identity=45.3453453453453, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI2367172, Length=278, Percent_Identity=38.4892086330935, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=274, Percent_Identity=37.956204379562, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=41.7721518987342, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI19921226, Length=373, Percent_Identity=33.5120643431635, Blast_Score=203, Evalue=1e-52,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40169; Mature: 40169

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHH
TGIAAEKILNTEKDLELIELAQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRP
CCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCEEEEEEEC
AAGGDESSIFVGNLFDTYRAYAENNNKMKIIEMTPNAVGFSFISFMISGEEVYSRMKFES
CCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHH
GVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNRT
HHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCEEEEEEEECCCCCCCCCCCC
ESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKLEAAQEQQNAEFANLRKNQVGTG
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
DRSEKIRTYNYPQNRVTDHRINLTLNKLDQIMMGELDEIIDALIADEQTGLMANLDI
CCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHH
TGIAAEKILNTEKDLELIELAQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRP
CCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCEEEEEEEC
AAGGDESSIFVGNLFDTYRAYAENNNKMKIIEMTPNAVGFSFISFMISGEEVYSRMKFES
CCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHH
GVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNRT
HHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCEEEEEEEECCCCCCCCCCCC
ESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKLEAAQEQQNAEFANLRKNQVGTG
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
DRSEKIRTYNYPQNRVTDHRINLTLNKLDQIMMGELDEIIDALIADEQTGLMANLDI
CCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA