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The map label for this gene is sppA [H]

Identifier: 170683467

GI number: 170683467

Start: 1409620

End: 1411488

Strand: Reverse

Name: sppA [H]

Synonym: EcSMS35_1425

Alternate gene names: 170683467

Gene position: 1411488-1409620 (Counterclockwise)

Preceding gene: 170681354

Following gene: 170682503

Centisome position: 27.85

GC content: 52.01

Gene sequence:

>1869_bases
TTGGGAGAATACATGCGAACCCTTTGGCGATTTATTGCCGGATTTTTTAAATGGACGTGGCGTCTGCTGAATTTCGTCCG
TGAAATGGTACTTAACCTGTTCTTTATTTTTCTCGTGCTGGTTGGTGTGGGGATCTGGATGCAGGTCAGTGGTGGTGATT
CGAAAGAAACGGCCAGTCGTGGCGCACTGCTGCTGGACATTTCTGGTGTGATCGTCGATAAACCCGACAGTTCTCAGCGG
TTTAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCACTGTTTGATATCGTCAACAC
CATTCGCCAGGCGAAGGACGACCGCAATATCACCGGTATCGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAGCCGT
CTATGCAGTACATCGGCAAAGCCCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGC
CAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCACCGCAAGGCGTGGTGGATCTGCACGGTTTTGC
CACCAACGGTCTGTACTACAAATCGTTACTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAAT
CTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGG
CAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTCAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGA
GGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCGCTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCCG
AAATCGAAAAAGCTCTGACCAAAGAGTTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTAC
GCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGAGTCGTCTTCGCTAATGGCGCAATTATGGATGGCGAGGAAAC
TCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTTC
TGCGTGTTAATAGTCCCGGCGGCAGCGTTACTGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGT
AAGCCTGTGGTTGTGTCGATGGGCGGTATGGCGGCATCTGGTGGTTACTGGATTTCCACACCAGCTAATTACATTGTGGC
TAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTG
TTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCACAGCAGATG
ATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCAACGCCAGAGCAGAT
TGATAAAATCGCCCAGGGCCACGTCTGGACCGGCCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCG
ATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATACTACGTTGATGAACCGACCTTC
TTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACC
GCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAACGACAAGCTGGCCGCGTTTAACGACCCACAAAACCGTTATGCGT
TTTGCCTGACCTGCGCCAACGTGCGTTAA

Upstream 100 bases:

>100_bases
GGATTTTGTTACAGCCCAGTCCGCAATTCCTGCTGACAAGTGCCGGTTGGGTCATTACGATAACCACATCTATTGCGCCT
GAAAAAGGTGTGACCTTAAG

Downstream 100 bases:

>100_bases
GTCATGTACTAAGTGGCCGACAGATCGTCGGCCACATTACTTTTTACGTCGACGAATCCTCTTCCCGCTGTCTCGCCCCA
TATTTCCTTATCCGCATAGT

Product: protease 4

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MGEYMRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQR
FSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYS
QGQYYLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELW
QNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDY
ALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAG
KPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQM
MQLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTF
FDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSENDKLAAFNDPQNRYAFCLTCANVR

Sequences:

>Translated_622_residues
MGEYMRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQR
FSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYS
QGQYYLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELW
QNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDY
ALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAG
KPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQM
MQLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTF
FDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSENDKLAAFNDPQNRYAFCLTCANVR
>Mature_621_residues
GEYMRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQRF
SKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQ
GQYYLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQ
NYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYA
LKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGK
PVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQMM
QLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFF
DKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSENDKLAAFNDPQNRYAFCLTCANVR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=618, Percent_Identity=99.3527508090615, Blast_Score=1261, Evalue=0.0,
Organism=Escherichia coli, GI1787527, Length=175, Percent_Identity=29.1428571428571, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 67709; Mature: 67578

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEYMRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHC
GALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGI
CEEEEEECCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGV
EEEHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
VDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELW
EEECCEECCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALT
HHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQI
HHCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCHHHHH
RDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWIST
HHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEEC
PANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQM
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHH
MQLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKA
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHH
AELAKVKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSEND
HHHHHHHHHHHHEEECCCHHHHHHHHCCCCCEEECCHHHHHHHCCCHHHHHHHHHHCCCC
KLAAFNDPQNRYAFCLTCANVR
CEEEECCCCCCEEEEEEECCCC
>Mature Secondary Structure 
GEYMRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHC
GALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGI
CEEEEEECCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGV
EEEHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
VDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELW
EEECCEECCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALT
HHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQI
HHCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCHHHHH
RDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWIST
HHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEEC
PANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQM
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHH
MQLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKA
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHH
AELAKVKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSEND
HHHHHHHHHHHHEEECCCHHHHHHHHCCCCCEEECCHHHHHHHCCCHHHHHHHHHHCCCC
KLAAFNDPQNRYAFCLTCANVR
CEEEECCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]