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The map label for this gene is gdhA [H]

Identifier: 170681354

GI number: 170681354

Start: 1415385

End: 1416728

Strand: Reverse

Name: gdhA [H]

Synonym: EcSMS35_1429

Alternate gene names: 170681354

Gene position: 1416728-1415385 (Counterclockwise)

Preceding gene: 170679665

Following gene: 170683467

Centisome position: 27.95

GC content: 53.05

Gene sequence:

>1344_bases
ATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGC
CGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTC
TGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGGTACAGGTCAACCGTGCATGG
CGTGTGCAGTTCAGTTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAA
ATTCCTCGGCTTCGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCG
ATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCG
GATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTC
CAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGATACG
GTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGAATGCGCGTTTCCGTTTCTGGCTCC
GGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGTGGCAC
TGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGG
CAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAACCGTGGTCTGTACCGGTTGATATCGCCCTGCCT
TGCGCCACCCAGAACGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCCAATGGCGTTAAAGCCGTCGCCGAAGGGGC
AAATATGCCGACCACCATCGAAGCGACTGAACTGTTCCAACAGGCAGGCGTACTGTTTGCACCGGGTAAAGCGGCTAATG
CTGGCGGTGTAGCAACATCGGGCCTGGAAATGGCGCAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCA
CGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCA
GGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCGGATGCGATGTTAAGCCAGGGCGTGATTTAA

Upstream 100 bases:

>100_bases
GCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAA
TATATAAGGGTTTTATATCT

Downstream 100 bases:

>100_bases
GACAATTGTGCCGGATACAGCGCAAACGCCTTATCCGGCCATAAAAATCTTCAGTATCAGATGGTTGAAAGAACCTTTAT
ATTATCACTCCATTGCCCGC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAW
RVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALP
CATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDA
RLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI

Sequences:

>Translated_447_residues
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAW
RVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALP
CATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDA
RLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI
>Mature_447_residues
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAW
RVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALP
CATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDA
RLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=406, Percent_Identity=30.2955665024631, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI4885281, Length=390, Percent_Identity=29.7435897435897, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788059, Length=447, Percent_Identity=99.3288590604027, Blast_Score=924, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=377, Percent_Identity=28.3819628647215, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=50.8849557522124, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=51.9823788546256, Blast_Score=420, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24649283, Length=319, Percent_Identity=32.2884012539185, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI45549226, Length=382, Percent_Identity=29.0575916230367, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45553475, Length=382, Percent_Identity=29.0575916230367, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24649460, Length=306, Percent_Identity=30.3921568627451, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48570; Mature: 48570

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEF
CCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQ
CEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEG
HCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EQTNYVQGANIAGFVKVADAMLSQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLVEPERV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMRVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRVADYAKEF
CCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQ
CEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEG
HCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EQTNYVQGANIAGFVKVADAMLSQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]