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The map label for this gene is ansA

Identifier: 170682503

GI number: 170682503

Start: 1408437

End: 1409453

Strand: Reverse

Name: ansA

Synonym: EcSMS35_1424

Alternate gene names: 170682503

Gene position: 1409453-1408437 (Counterclockwise)

Preceding gene: 170683467

Following gene: 170683713

Centisome position: 27.81

GC content: 50.44

Gene sequence:

>1017_bases
ATGCAAAAGAAATCAATTTACGTCGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATTCCGGT
GTCAGGTCATCTACAGCGCCAACTGGCGCTGATGCCGGAATTCCATCGCCCGGAGATGCCAGATTTCACCATTCATGAAT
ATACGCCGCTGATGGACTCTTCAGATATGACGCCAGAAGACTGGCAGCATATTGCTGAAGATATTAAAGCGCATTATGAC
GACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAA
TCTCGGTAAACCGGTTATTGTGACAGGGTCACAAATCCCGCTAGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGA
ATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCTTTTTTAATAATCGACTTTATCGTGGCAACCGT
ACCACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGCCTCTCCAAACCTTCCTCCGTTACTGGAAGCAGGTATCCATAT
TCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTGATCGTTCATCCAATCACCCCACAACCGATTGGCG
TAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAATTTTCTGCGCCAACCGGTGAAAGCATTGATTCTGCGC
TCATATGGCGTGGGTAATGCGCCACAAAACAAAGCCTTCCTGCAAGAATTACAAGAAGCCAGCGATCGCGGAATTGTGGT
AGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGGGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCG
TCATTGGCGGTGCAGATATGACTGTAGAAGCCACACTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAA
ACCATTCGCAAGGCCATGAGCCAAAACCTGCGTGGCGAACTGACGCCGGATGATTAA

Upstream 100 bases:

>100_bases
GCTGTCTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCG
ATATCATTCATATCAATATC

Downstream 100 bases:

>100_bases
GGAGACTGCAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGC
CGGAAGGTGACAGTACGGTG

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYD
DYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTE
TIRKAMSQNLRGELTPDD

Sequences:

>Translated_338_residues
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYD
DYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTE
TIRKAMSQNLRGELTPDD
>Mature_338_residues
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYD
DYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTE
TIRKAMSQNLRGELTPDD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family

Homologues:

Organism=Homo sapiens, GI122937331, Length=363, Percent_Identity=45.1790633608815, Blast_Score=279, Evalue=2e-75,
Organism=Escherichia coli, GI1788065, Length=338, Percent_Identity=100, Blast_Score=699, Evalue=0.0,
Organism=Escherichia coli, GI1789327, Length=282, Percent_Identity=28.0141843971631, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=358, Percent_Identity=45.2513966480447, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI193207372, Length=361, Percent_Identity=44.8753462603878, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI193207374, Length=358, Percent_Identity=45.2513966480447, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17558278, Length=358, Percent_Identity=45.2513966480447, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24639609, Length=366, Percent_Identity=44.2622950819672, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24645530, Length=362, Percent_Identity=43.646408839779, Blast_Score=279, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASPG1_ECO57 (P0A963)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90938
- RefSeq:   NP_288201.1
- RefSeq:   NP_310501.1
- ProteinModelPortal:   P0A963
- SMR:   P0A963
- EnsemblBacteria:   EBESCT00000026572
- EnsemblBacteria:   EBESCT00000056644
- GeneID:   916909
- GeneID:   961739
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2801
- KEGG:   ecs:ECs2474
- GeneTree:   EBGT00050000011153
- HOGENOM:   HBG497495
- OMA:   PEFYHDE
- ProtClustDB:   PRK09461
- BioCyc:   ECOL83334:ECS2474-MONOMER
- GO:   GO:0005737
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: =3.5.1.1

Molecular weight: Translated: 37128; Mature: 37128

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 14-14

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCC
SDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
HHCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTPDD
EHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCC
SDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
HHCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTPDD
EHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796