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The map label for this gene is rfaF [H]

Identifier: 170026354

GI number: 170026354

Start: 4579861

End: 4580925

Strand: Direct

Name: rfaF [H]

Synonym: YPK_4147

Alternate gene names: 170026354

Gene position: 4579861-4580925 (Clockwise)

Preceding gene: 170026353

Following gene: 170026355

Centisome position: 97.66

GC content: 51.83

Gene sequence:

>1065_bases
ATGAAAATACTGGTCATCGGCCCTTCATGGGTTGGCGATATGATGATGTCGCAAAGTCTTTACCGTACCCTGAAGGCCGA
ATATCCGGCAGCAGACATTGATGTGATGGCACCCGCATGGTGCCGTCCGCTTCTGGCCAGAATGCCGGAAGTCCGTCACG
CAATTCCAATGCCGTTAGGCCATGGGGCATTTGCTTTTGAGGAGCGTCGCCGTTTGGGATTGGCCTTACGTGAAACCGAG
TATGATCGTGCTTACGTGTTGCCCAACTCATTTAAATCCGCGCTGATACCTTACTTTTCAGGGATCAAACGACGTATTGG
CTGGCGTGGCGAAATGCGTTATTTCCTGCTCAATGACCTCCGTATTCTGGATAAGCAAGCCTTTCCAATGATGGTCCAGC
GCTACGTTGCGCTGGCCTATGATAAAGAACGCATCCGTTCAGCGGCAGATTTACCACAACCGTTGTTGTGGCCGCAATTG
CAAGTACGCGATGAAGAAATTGCAGAGACAACGGCCTCATTCAATCTGACCGATAACCGGCCCATTATTGGATTCTGCCC
TGGCGCAGAATTTGGGCCAGCGAAACGTTGGCCGCATTACCACTACGCCACGCTGGCGCAGAAATTGATTGATGATGGCT
ATCAGGTCGTCTTATTCGGTTCTGCGAAAGACAATGAAGCTGGTGAAGATATCCGTCAGGCTCTCAGCGATACAGATCGT
GAATATTGCCTCAATCTGGCAGGGCAGACCTCACTGGAACAAGCGGTCGTTCTCATCGCCGCCTGTAGTGCCGTCGTGAG
TAACGACTCTGGTTTAATGCATGTCGCGGCGGCACTCAACAAGCCATTAGTGGCATTGTATGGCCCGAGCAGCCCAGACT
TCACGCCACCACTGTCAGAGAAAGCCACGGTGATCCGTCTGATTACCGGCTATCACAAAGTCCGCAAGGGCGATTCTGCA
CAGGGCTATCATCAAAGTTTGATTGATATCCAACCCGAACACGTGATGGCCGCGCTTGAGAAGCAATTGGCTACGCAAGA
ATGCTCAGTCAAAGGGGGCGATTAA

Upstream 100 bases:

>100_bases
TGGTAAACCGTTTAAAACGGTCGCTGAAGGCGTTAAAGAATATCTGGCCTGGCTCAATCGCTCAGTCTAAATCACTTGCT
GCAAGGAATTGATAAACGGT

Downstream 100 bases:

>100_bases
TGCACGTATTAATCGTTAAAACCTCGTCAATGGGCGATGTTTTACATACCTTACCCGCGCTGACGGATGCCATGAATGCC
ATACCCGGTATTCGCTTCGA

Product: ADP-heptose:LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKILVIGPSWVGDMMMSQSLYRTLKAEYPAADIDVMAPAWCRPLLARMPEVRHAIPMPLGHGAFAFEERRRLGLALRETE
YDRAYVLPNSFKSALIPYFSGIKRRIGWRGEMRYFLLNDLRILDKQAFPMMVQRYVALAYDKERIRSAADLPQPLLWPQL
QVRDEEIAETTASFNLTDNRPIIGFCPGAEFGPAKRWPHYHYATLAQKLIDDGYQVVLFGSAKDNEAGEDIRQALSDTDR
EYCLNLAGQTSLEQAVVLIAACSAVVSNDSGLMHVAAALNKPLVALYGPSSPDFTPPLSEKATVIRLITGYHKVRKGDSA
QGYHQSLIDIQPEHVMAALEKQLATQECSVKGGD

Sequences:

>Translated_354_residues
MKILVIGPSWVGDMMMSQSLYRTLKAEYPAADIDVMAPAWCRPLLARMPEVRHAIPMPLGHGAFAFEERRRLGLALRETE
YDRAYVLPNSFKSALIPYFSGIKRRIGWRGEMRYFLLNDLRILDKQAFPMMVQRYVALAYDKERIRSAADLPQPLLWPQL
QVRDEEIAETTASFNLTDNRPIIGFCPGAEFGPAKRWPHYHYATLAQKLIDDGYQVVLFGSAKDNEAGEDIRQALSDTDR
EYCLNLAGQTSLEQAVVLIAACSAVVSNDSGLMHVAAALNKPLVALYGPSSPDFTPPLSEKATVIRLITGYHKVRKGDSA
QGYHQSLIDIQPEHVMAALEKQLATQECSVKGGD
>Mature_354_residues
MKILVIGPSWVGDMMMSQSLYRTLKAEYPAADIDVMAPAWCRPLLARMPEVRHAIPMPLGHGAFAFEERRRLGLALRETE
YDRAYVLPNSFKSALIPYFSGIKRRIGWRGEMRYFLLNDLRILDKQAFPMMVQRYVALAYDKERIRSAADLPQPLLWPQL
QVRDEEIAETTASFNLTDNRPIIGFCPGAEFGPAKRWPHYHYATLAQKLIDDGYQVVLFGSAKDNEAGEDIRQALSDTDR
EYCLNLAGQTSLEQAVVLIAACSAVVSNDSGLMHVAAALNKPLVALYGPSSPDFTPPLSEKATVIRLITGYHKVRKGDSA
QGYHQSLIDIQPEHVMAALEKQLATQECSVKGGD

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=347, Percent_Identity=74.0634005763689, Blast_Score=546, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 39556; Mature: 39556

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGPSWVGDMMMSQSLYRTLKAEYPAADIDVMAPAWCRPLLARMPEVRHAIPMPLG
CEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCC
HGAFAFEERRRLGLALRETEYDRAYVLPNSFKSALIPYFSGIKRRIGWRGEMRYFLLNDL
CCCHHHHHHHHHCCHHECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RILDKQAFPMMVQRYVALAYDKERIRSAADLPQPLLWPQLQVRDEEIAETTASFNLTDNR
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHCEEECCCC
PIIGFCPGAEFGPAKRWPHYHYATLAQKLIDDGYQVVLFGSAKDNEAGEDIRQALSDTDR
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCH
EYCLNLAGQTSLEQAVVLIAACSAVVSNDSGLMHVAAALNKPLVALYGPSSPDFTPPLSE
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCC
KATVIRLITGYHKVRKGDSAQGYHQSLIDIQPEHVMAALEKQLATQECSVKGGD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKILVIGPSWVGDMMMSQSLYRTLKAEYPAADIDVMAPAWCRPLLARMPEVRHAIPMPLG
CEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCC
HGAFAFEERRRLGLALRETEYDRAYVLPNSFKSALIPYFSGIKRRIGWRGEMRYFLLNDL
CCCHHHHHHHHHCCHHECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RILDKQAFPMMVQRYVALAYDKERIRSAADLPQPLLWPQLQVRDEEIAETTASFNLTDNR
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHCEEECCCC
PIIGFCPGAEFGPAKRWPHYHYATLAQKLIDDGYQVVLFGSAKDNEAGEDIRQALSDTDR
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCH
EYCLNLAGQTSLEQAVVLIAACSAVVSNDSGLMHVAAALNKPLVALYGPSSPDFTPPLSE
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCC
KATVIRLITGYHKVRKGDSAQGYHQSLIDIQPEHVMAALEKQLATQECSVKGGD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]