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The map label for this gene is rfaD [H]

Identifier: 170026353

GI number: 170026353

Start: 4578898

End: 4579830

Strand: Direct

Name: rfaD [H]

Synonym: YPK_4146

Alternate gene names: 170026353

Gene position: 4578898-4579830 (Clockwise)

Preceding gene: 170026350

Following gene: 170026354

Centisome position: 97.64

GC content: 45.12

Gene sequence:

>933_bases
ATGATTATCGTTACAGGCGGCGCAGGTTTCATTGGCAGCAATATAGTTAAGGCTCTGAATAATATTGGCTACAAAGATAT
TCTGGTGGTTGATAACCTCAAAGACGGCACTAAATTCGTCAATCTGGTTGATCTGGATATCGCTGATTACATGGATAAAG
AAGATTTTGTCGCCAGTATTGTTGCGGGTGATGACATGGGCGATATCGATGCTATTTTCCACGAAGGCGCGTGTTCTTCC
ACCACCGAGTGGGATGGCAAGTACATGATGGATAACAACTATCAGTATTCTAAAGATATCCTTCACTTCTGTCTGGATCG
TAGTATTCCTTTCTTATACGCCTCTTCTGCCGCCACTTACGGTGGGCGCACTGACAACTTTATTGAAGATCGTCAGTACG
AACAGCCACTTAACGTTTATGGCTATTCCAAATTCTTGTTTGATCAGTATGTGCGTGAAATTCTGCCACAAGCCGATTCG
CAAATTTGTGGATTCCGTTATTTCAACGTTTATGGTCCACGTGAAGGCCATAAGGGCAGCATGGCCAGCGTCGCTTTCCA
TCTGAATAACCAAATTAATGCAGGTGAAAGGCCTAAGTTATTCGCGGGTAGCGAAAACTTTAAACGTGATTTCATTTATG
TGGGTGATGTTGCTGACGTTAATCTGTGGTTCTGGCAAAACGGCGTATCCGGTATTTTCAACTGCGGTACTGGCCGGGCA
GAGTCTTTCCAGGCTGTCGCTGATGCAGTGGTGGATTATCACCAAAGTGGGCCAGTCGAATACATCGAGTTCCCTGAAAA
ACTGAAAGGCCGCTATCAGGCATATACACAAGCCGATCTCACTAAATTACGGGCTGCCGGTTATGGTAAACCGTTTAAAA
CGGTCGCTGAAGGCGTTAAAGAATATCTGGCCTGGCTCAATCGCTCAGTCTAA

Upstream 100 bases:

>100_bases
TCCCCTTCCCGCTGTGCGCTATCCTATGAAATGTTATGATAGTGCCAAATAGGGGGTGGGGTGTGGTACCCAACCAAGAA
AATTAGCAAGGGTGAATACG

Downstream 100 bases:

>100_bases
ATCACTTGCTGCAAGGAATTGATAAACGGTATGAAAATACTGGTCATCGGCCCTTCATGGGTTGGCGATATGATGATGTC
GCAAAGTCTTTACCGTACCC

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIIVTGGAGFIGSNIVKALNNIGYKDILVVDNLKDGTKFVNLVDLDIADYMDKEDFVASIVAGDDMGDIDAIFHEGACSS
TTEWDGKYMMDNNYQYSKDILHFCLDRSIPFLYASSAATYGGRTDNFIEDRQYEQPLNVYGYSKFLFDQYVREILPQADS
QICGFRYFNVYGPREGHKGSMASVAFHLNNQINAGERPKLFAGSENFKRDFIYVGDVADVNLWFWQNGVSGIFNCGTGRA
ESFQAVADAVVDYHQSGPVEYIEFPEKLKGRYQAYTQADLTKLRAAGYGKPFKTVAEGVKEYLAWLNRSV

Sequences:

>Translated_310_residues
MIIVTGGAGFIGSNIVKALNNIGYKDILVVDNLKDGTKFVNLVDLDIADYMDKEDFVASIVAGDDMGDIDAIFHEGACSS
TTEWDGKYMMDNNYQYSKDILHFCLDRSIPFLYASSAATYGGRTDNFIEDRQYEQPLNVYGYSKFLFDQYVREILPQADS
QICGFRYFNVYGPREGHKGSMASVAFHLNNQINAGERPKLFAGSENFKRDFIYVGDVADVNLWFWQNGVSGIFNCGTGRA
ESFQAVADAVVDYHQSGPVEYIEFPEKLKGRYQAYTQADLTKLRAAGYGKPFKTVAEGVKEYLAWLNRSV
>Mature_310_residues
MIIVTGGAGFIGSNIVKALNNIGYKDILVVDNLKDGTKFVNLVDLDIADYMDKEDFVASIVAGDDMGDIDAIFHEGACSS
TTEWDGKYMMDNNYQYSKDILHFCLDRSIPFLYASSAATYGGRTDNFIEDRQYEQPLNVYGYSKFLFDQYVREILPQADS
QICGFRYFNVYGPREGHKGSMASVAFHLNNQINAGERPKLFAGSENFKRDFIYVGDVADVNLWFWQNGVSGIFNCGTGRA
ESFQAVADAVVDYHQSGPVEYIEFPEKLKGRYQAYTQADLTKLRAAGYGKPFKTVAEGVKEYLAWLNRSV

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=308, Percent_Identity=83.1168831168831, Blast_Score=547, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71982038, Length=287, Percent_Identity=26.8292682926829, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=285, Percent_Identity=27.0175438596491, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 34780; Mature: 34780

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGFIGSNIVKALNNIGYKDILVVDNLKDGTKFVNLVDLDIADYMDKEDFVASI
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEHHHHHCCHHHHHHHH
VAGDDMGDIDAIFHEGACSSTTEWDGKYMMDNNYQYSKDILHFCLDRSIPFLYASSAATY
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCC
GGRTDNFIEDRQYEQPLNVYGYSKFLFDQYVREILPQADSQICGFRYFNVYGPREGHKGS
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCC
MASVAFHLNNQINAGERPKLFAGSENFKRDFIYVGDVADVNLWFWQNGVSGIFNCGTGRA
CEEEEEEECCCCCCCCCCCEEECCCCCCCCEEEECCCCEEEEEEECCCCCCEEECCCCCH
ESFQAVADAVVDYHQSGPVEYIEFPEKLKGRYQAYTQADLTKLRAAGYGKPFKTVAEGVK
HHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EYLAWLNRSV
HHHHHHCCCC
>Mature Secondary Structure
MIIVTGGAGFIGSNIVKALNNIGYKDILVVDNLKDGTKFVNLVDLDIADYMDKEDFVASI
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEHHHHHCCHHHHHHHH
VAGDDMGDIDAIFHEGACSSTTEWDGKYMMDNNYQYSKDILHFCLDRSIPFLYASSAATY
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCC
GGRTDNFIEDRQYEQPLNVYGYSKFLFDQYVREILPQADSQICGFRYFNVYGPREGHKGS
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCC
MASVAFHLNNQINAGERPKLFAGSENFKRDFIYVGDVADVNLWFWQNGVSGIFNCGTGRA
CEEEEEEECCCCCCCCCCCEEECCCCCCCCEEEECCCCEEEEEEECCCCCCEEECCCCCH
ESFQAVADAVVDYHQSGPVEYIEFPEKLKGRYQAYTQADLTKLRAAGYGKPFKTVAEGVK
HHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EYLAWLNRSV
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA