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The map label for this gene is hofB [H]

Identifier: 170025714

GI number: 170025714

Start: 3845308

End: 3846846

Strand: Direct

Name: hofB [H]

Synonym: YPK_3499

Alternate gene names: 170025714

Gene position: 3845308-3846846 (Clockwise)

Preceding gene: 170025713

Following gene: 170025715

Centisome position: 82.0

GC content: 46.07

Gene sequence:

>1539_bases
ATGGCTGAATTTATAGTGTCCTCATTTGAAACAATTAGTGATGAATTACACCTTCTCTGTCGGCGTTATCGGGCAGTGGC
ACTCACTCTTGATGGTAAAAGCCTTTCGATAGCTTCAGCCCAGACCGTTGATGAAGCGTTATTGACCGCGTTGCGTTTCA
CATGTGGCCGTCAAGTCAGAGTGGAATATTGGCCCGAAGCCAAGATTGAACAATCATTGTATTTGGGGAGCCCGACGAAA
AATAACCTAAACAGTAGTATTAACAACGGGCTAAATAGCGCGTTGTTATCCCAAAAGGGAGGTGATCCGTCGCGAGTAAA
ACCGCACAATCAGCAGCAACAGACAATAGATGATACTGTGTTTGATAATGAGAGTGACACACCCGTCATTCAATTCATAA
CCCAGACGCTAAGTCTAGCCATTCAAAAACGCGCTTCAGATATCCATTTTGAGCCTTATCAACACCACTATCGTGTTCGT
TTAAGAATTGATGGTGTGTTGCATGAATTCACCCCACCCGAGGCCGAATGGGCAGCTCGGATTAGCAGTTGCCTGAAGGT
CATGGCGAAATTAAATATTGCTGAACGGCGATTACCACAAGATGGTCAACTAACCTTACCCTTTGGTGATTCACACTATT
CAATGCGGATAGCGACTCTCCCTACGCAATATGGTGAAAAAGTGGTATTGCGTATTCTTCAAATACAACAGCAAACCACG
TTAGAAAAGCTGGGGATGACGGATGCGGCACTGAAACAATTAACACAGGCATTATCAGCACCACAAGGGCTGATTCTGGT
CACCGGCCCTACCGGTAGTGGCAAAACCATTACGTTATATTGCAGTTTAGCGCGGCTGAATCAGACACAAAGAAACATCT
GTAGCGTCGAAGATCCTATTGAGATCCCCGTCAATGGCATTAACCAAACCCAGGTAAACAGCAAGATCGGTCTGGATTTC
TCTCGAATACTACGAGCCATCCTACGTCAGGACCCTGATGTCATTATGGTTGGTGAAATTCGTGATAATGAAACCGCCAG
TATCGCAGTTAACGCGGCCCAGACCGGGCATTTGGTCCTATCGACGCTGCACACTAACTCAACAGCAGAAACGCTGATAC
GCATGGCACAAATGGGAATAGAACGCCATTTAATCGCCTCAAGTCTAAAACTCGTCATTGCTCAACGCTTGGTGCGCCGC
CTATGTTTACATTGCCGCCAGGCTGCATCTCACCCCTTTATTCCACCAGCTCACATAAGGTCTGGTCCGATCCAACACTA
TCTAGCTGTAGGTTGTGAGCATTGTTGTACGGGTTATTATGGCCGAACGGGTATTTATGAAATGCTGAGTGTAACGCCGC
AGATTCAGCAAGCCATACTCAATAATGCCAGCCCTGTAAAACTGGTACAAATTGCCCAGAAGCAAGAACAAACAGCCTTA
CTCTGCTCAGGTTTAGCTTTAATCGAAAAAGGCATCACTACCCTTAGTGAAATTAATCGTGTTGTGGGCTTCGTAGCAGA
AACAGAGGTCACCTCTTGA

Upstream 100 bases:

>100_bases
AACGGAAATTTAGTCTGGGCCAGAACATGTACGGCAACAGATAGTGCCATGACTGATAGTTGTAAAGCGGTATTCCGTTT
CAATGATAAGGCCCCATCAA

Downstream 100 bases:

>100_bases
GCCGCCATCGTTTATTCAATTGGACAGCTCTCAACAAAACAGGGGAGCTACAGACGGGCATGCTACTGGCAACTGAGAGA
AACAGTGTCTATGAACATAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAEFIVSSFETISDELHLLCRRYRAVALTLDGKSLSIASAQTVDEALLTALRFTCGRQVRVEYWPEAKIEQSLYLGSPTK
NNLNSSINNGLNSALLSQKGGDPSRVKPHNQQQQTIDDTVFDNESDTPVIQFITQTLSLAIQKRASDIHFEPYQHHYRVR
LRIDGVLHEFTPPEAEWAARISSCLKVMAKLNIAERRLPQDGQLTLPFGDSHYSMRIATLPTQYGEKVVLRILQIQQQTT
LEKLGMTDAALKQLTQALSAPQGLILVTGPTGSGKTITLYCSLARLNQTQRNICSVEDPIEIPVNGINQTQVNSKIGLDF
SRILRAILRQDPDVIMVGEIRDNETASIAVNAAQTGHLVLSTLHTNSTAETLIRMAQMGIERHLIASSLKLVIAQRLVRR
LCLHCRQAASHPFIPPAHIRSGPIQHYLAVGCEHCCTGYYGRTGIYEMLSVTPQIQQAILNNASPVKLVQIAQKQEQTAL
LCSGLALIEKGITTLSEINRVVGFVAETEVTS

Sequences:

>Translated_512_residues
MAEFIVSSFETISDELHLLCRRYRAVALTLDGKSLSIASAQTVDEALLTALRFTCGRQVRVEYWPEAKIEQSLYLGSPTK
NNLNSSINNGLNSALLSQKGGDPSRVKPHNQQQQTIDDTVFDNESDTPVIQFITQTLSLAIQKRASDIHFEPYQHHYRVR
LRIDGVLHEFTPPEAEWAARISSCLKVMAKLNIAERRLPQDGQLTLPFGDSHYSMRIATLPTQYGEKVVLRILQIQQQTT
LEKLGMTDAALKQLTQALSAPQGLILVTGPTGSGKTITLYCSLARLNQTQRNICSVEDPIEIPVNGINQTQVNSKIGLDF
SRILRAILRQDPDVIMVGEIRDNETASIAVNAAQTGHLVLSTLHTNSTAETLIRMAQMGIERHLIASSLKLVIAQRLVRR
LCLHCRQAASHPFIPPAHIRSGPIQHYLAVGCEHCCTGYYGRTGIYEMLSVTPQIQQAILNNASPVKLVQIAQKQEQTAL
LCSGLALIEKGITTLSEINRVVGFVAETEVTS
>Mature_511_residues
AEFIVSSFETISDELHLLCRRYRAVALTLDGKSLSIASAQTVDEALLTALRFTCGRQVRVEYWPEAKIEQSLYLGSPTKN
NLNSSINNGLNSALLSQKGGDPSRVKPHNQQQQTIDDTVFDNESDTPVIQFITQTLSLAIQKRASDIHFEPYQHHYRVRL
RIDGVLHEFTPPEAEWAARISSCLKVMAKLNIAERRLPQDGQLTLPFGDSHYSMRIATLPTQYGEKVVLRILQIQQQTTL
EKLGMTDAALKQLTQALSAPQGLILVTGPTGSGKTITLYCSLARLNQTQRNICSVEDPIEIPVNGINQTQVNSKIGLDFS
RILRAILRQDPDVIMVGEIRDNETASIAVNAAQTGHLVLSTLHTNSTAETLIRMAQMGIERHLIASSLKLVIAQRLVRRL
CLHCRQAASHPFIPPAHIRSGPIQHYLAVGCEHCCTGYYGRTGIYEMLSVTPQIQQAILNNASPVKLVQIAQKQEQTALL
CSGLALIEKGITTLSEINRVVGFVAETEVTS

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=491, Percent_Identity=45.213849287169, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789723, Length=403, Percent_Identity=47.6426799007444, Blast_Score=351, Evalue=8e-98,
Organism=Escherichia coli, GI87082188, Length=278, Percent_Identity=33.4532374100719, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 56599; Mature: 56468

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFIVSSFETISDELHLLCRRYRAVALTLDGKSLSIASAQTVDEALLTALRFTCGRQVR
CCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCEE
VEYWPEAKIEQSLYLGSPTKNNLNSSINNGLNSALLSQKGGDPSRVKPHNQQQQTIDDTV
EEECCCCHHCCCEEECCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
FDNESDTPVIQFITQTLSLAIQKRASDIHFEPYQHHYRVRLRIDGVLHEFTPPEAEWAAR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECHHHCCCCCHHHHHHH
ISSCLKVMAKLNIAERRLPQDGQLTLPFGDSHYSMRIATLPTQYGEKVVLRILQIQQQTT
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
LEKLGMTDAALKQLTQALSAPQGLILVTGPTGSGKTITLYCSLARLNQTQRNICSVEDPI
HHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHHCHHHHHHCCCCCCC
EIPVNGINQTQVNSKIGLDFSRILRAILRQDPDVIMVGEIRDNETASIAVNAAQTGHLVL
CCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCEEE
STLHTNSTAETLIRMAQMGIERHLIASSLKLVIAQRLVRRLCLHCRQAASHPFIPPAHIR
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC
SGPIQHYLAVGCEHCCTGYYGRTGIYEMLSVTPQIQQAILNNASPVKLVQIAQKQEQTAL
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LCSGLALIEKGITTLSEINRVVGFVAETEVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEFIVSSFETISDELHLLCRRYRAVALTLDGKSLSIASAQTVDEALLTALRFTCGRQVR
CHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCEE
VEYWPEAKIEQSLYLGSPTKNNLNSSINNGLNSALLSQKGGDPSRVKPHNQQQQTIDDTV
EEECCCCHHCCCEEECCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
FDNESDTPVIQFITQTLSLAIQKRASDIHFEPYQHHYRVRLRIDGVLHEFTPPEAEWAAR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECHHHCCCCCHHHHHHH
ISSCLKVMAKLNIAERRLPQDGQLTLPFGDSHYSMRIATLPTQYGEKVVLRILQIQQQTT
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
LEKLGMTDAALKQLTQALSAPQGLILVTGPTGSGKTITLYCSLARLNQTQRNICSVEDPI
HHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHHCHHHHHHCCCCCCC
EIPVNGINQTQVNSKIGLDFSRILRAILRQDPDVIMVGEIRDNETASIAVNAAQTGHLVL
CCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCEEE
STLHTNSTAETLIRMAQMGIERHLIASSLKLVIAQRLVRRLCLHCRQAASHPFIPPAHIR
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC
SGPIQHYLAVGCEHCCTGYYGRTGIYEMLSVTPQIQQAILNNASPVKLVQIAQKQEQTAL
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LCSGLALIEKGITTLSEINRVVGFVAETEVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959070; 8202364; 9278503 [H]