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The map label for this gene is proA [H]

Identifier: 170025504

GI number: 170025504

Start: 3597278

End: 3598537

Strand: Reverse

Name: proA [H]

Synonym: YPK_3287

Alternate gene names: 170025504

Gene position: 3598537-3597278 (Counterclockwise)

Preceding gene: 170025505

Following gene: 170025503

Centisome position: 76.74

GC content: 50.63

Gene sequence:

>1260_bases
ATGAACCTGTTAGAACATATGGGAAAAGCGGCCAAACAGGCTTCCTGGCAATTGGCGATGTTGAGTACGGCCAAAAAAAA
TCAGGCACTGGCGGTCATAGCTAACCTATTAGAATCAGAGAGCCAAACTATTTTGCAGGCCAATGAGCGAGATATGGCTG
CAGCACGCGAGAGTGGCATGAGCGAGGCGCTACTGGATCGCCTGTTATTGACGCCCGCCCGCTTAGCCGCCATTGCTAAT
GATGTACGTCAGGTATGCCGCCTAAACGATCCTGTAGGGCGTGTTATTGATGGCAGTTTGCTGGACAGTGGTTTAAAACT
GGAACGTCGACGTGTCCCGCTGGGGGTGATTGGTGTCATTTATGAAGCTCGCCCTAATGTTACTATCGATGTCGCTAGCC
TGTGTCTGAAAACTGGCAATGCGGTGATTTTGCGCGGTGGTAAAGAAACGCATCATACCAATCAGGCGACAGTAAATGTT
ATTCAGCGGGCGCTGGAGCAATGTGGTCTACCGGCCGCTGCAGTTCAGGCCATTGAAAGCCCAGACCGCCAACTGGTGAA
TGAACTGTTACGTCTGGATCGCTATGTGGATATGTTGATTCCACGCGGCGGCGCAAGCTTACATAAATTGTGCCGAGAGC
AATCGACCATCCCGGTTATTACCGGGGGTATTGGTGTTTGCCATACCTTTGTTGATGAAAACGCGGATTTTGAAAAAGCC
TTATTGGTGATCGAAAACGCGAAGATACAACGGCCAAGTGCTTGTAACTCACTGGAGACGTTGCTGGTTCATCAGGCGGT
TGCCAAAACTTTCCTGCCGCTATTAAGTGCCAGAATGCACGCGTTTGGCGTGACATTGCATGCCAGTCCGCTGGCGATGC
CCTATCTGGCAGATGGAAAAGCCAAAGTTGTGGCAGTAGAAGCCGCTGATTACGATGATGAATGGCTATCACTGGATCTA
AATGTGGATATCGTTACTGATATTGATGCCGCCATTGACCATATCCGTGAGCATGGCACTAGCCACTCTGATGCTATCCT
GACCCGTTCCCTGAGCCATGCTGAGTATTTTGTCCGCGCTGTCGATTCATCGGCTGTTTATGTTAACGCGAGCACTCGGT
TCACCGATGGCGGTCAGTTTGGTTTGGGTGCAGAAGTGGCAGTGAGCACACAAAAACTCCATGCCCGAGGCCCTATGGGA
CTGGATGCGCTGACGACGTATAAATGGATAGGTTACGGCGACGATTTAGTTCGTAGCTGA

Upstream 100 bases:

>100_bases
AGCGGGGCATCACTCGCAACAAATCAGTGAAATCCTCGGATATGAATACGGCCCGGTGGCTGTTCATCGTGATGATATGA
TTGTCAGCTAAGGAGCAAAC

Downstream 100 bases:

>100_bases
TTTATTTTTTAGTAGGTTTATTGGCAACAGGTTTATTCTTAACCGGTTGATCAATAGCTGATTGATTAAAAATCGCTAGT
AAAGCGACATGTGGCAATTT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNLLEHMGKAAKQASWQLAMLSTAKKNQALAVIANLLESESQTILQANERDMAAARESGMSEALLDRLLLTPARLAAIAN
DVRQVCRLNDPVGRVIDGSLLDSGLKLERRRVPLGVIGVIYEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVNV
IQRALEQCGLPAAAVQAIESPDRQLVNELLRLDRYVDMLIPRGGASLHKLCREQSTIPVITGGIGVCHTFVDENADFEKA
LLVIENAKIQRPSACNSLETLLVHQAVAKTFLPLLSARMHAFGVTLHASPLAMPYLADGKAKVVAVEAADYDDEWLSLDL
NVDIVTDIDAAIDHIREHGTSHSDAILTRSLSHAEYFVRAVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMG
LDALTTYKWIGYGDDLVRS

Sequences:

>Translated_419_residues
MNLLEHMGKAAKQASWQLAMLSTAKKNQALAVIANLLESESQTILQANERDMAAARESGMSEALLDRLLLTPARLAAIAN
DVRQVCRLNDPVGRVIDGSLLDSGLKLERRRVPLGVIGVIYEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVNV
IQRALEQCGLPAAAVQAIESPDRQLVNELLRLDRYVDMLIPRGGASLHKLCREQSTIPVITGGIGVCHTFVDENADFEKA
LLVIENAKIQRPSACNSLETLLVHQAVAKTFLPLLSARMHAFGVTLHASPLAMPYLADGKAKVVAVEAADYDDEWLSLDL
NVDIVTDIDAAIDHIREHGTSHSDAILTRSLSHAEYFVRAVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMG
LDALTTYKWIGYGDDLVRS
>Mature_419_residues
MNLLEHMGKAAKQASWQLAMLSTAKKNQALAVIANLLESESQTILQANERDMAAARESGMSEALLDRLLLTPARLAAIAN
DVRQVCRLNDPVGRVIDGSLLDSGLKLERRRVPLGVIGVIYEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVNV
IQRALEQCGLPAAAVQAIESPDRQLVNELLRLDRYVDMLIPRGGASLHKLCREQSTIPVITGGIGVCHTFVDENADFEKA
LLVIENAKIQRPSACNSLETLLVHQAVAKTFLPLLSARMHAFGVTLHASPLAMPYLADGKAKVVAVEAADYDDEWLSLDL
NVDIVTDIDAAIDHIREHGTSHSDAILTRSLSHAEYFVRAVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMG
LDALTTYKWIGYGDDLVRS

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=421, Percent_Identity=37.5296912114014, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI21361368, Length=421, Percent_Identity=37.5296912114014, Blast_Score=275, Evalue=5e-74,
Organism=Escherichia coli, GI1786438, Length=417, Percent_Identity=72.4220623501199, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=36.3855421686747, Blast_Score=267, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=36.3855421686747, Blast_Score=267, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6324899, Length=436, Percent_Identity=38.302752293578, Blast_Score=296, Evalue=4e-81,
Organism=Drosophila melanogaster, GI21357643, Length=415, Percent_Identity=37.3493975903614, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45317; Mature: 45317

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLEHMGKAAKQASWQLAMLSTAKKNQALAVIANLLESESQTILQANERDMAAARESGM
CCHHHHHHHHHHHHCCEEHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCH
SEALLDRLLLTPARLAAIANDVRQVCRLNDPVGRVIDGSLLDSGLKLERRRVPLGVIGVI
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHCCCHHHHHCCCEEEEEEE
YEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVNVIQRALEQCGLPAAAVQAIES
EECCCCCEEHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCC
PDRQLVNELLRLDRYVDMLIPRGGASLHKLCREQSTIPVITGGIGVCHTFVDENADFEKA
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCHHHE
LLVIENAKIQRPSACNSLETLLVHQAVAKTFLPLLSARMHAFGVTLHASPLAMPYLADGK
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEECCCC
AKVVAVEAADYDDEWLSLDLNVDIVTDIDAAIDHIREHGTSHSDAILTRSLSHAEYFVRA
EEEEEEEECCCCCCEEEEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLDALTTYKWIGYGDDLVRS
CCCCEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCCHHHHHEEECCCCHHHHCC
>Mature Secondary Structure
MNLLEHMGKAAKQASWQLAMLSTAKKNQALAVIANLLESESQTILQANERDMAAARESGM
CCHHHHHHHHHHHHCCEEHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCH
SEALLDRLLLTPARLAAIANDVRQVCRLNDPVGRVIDGSLLDSGLKLERRRVPLGVIGVI
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHCCCHHHHHCCCEEEEEEE
YEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVNVIQRALEQCGLPAAAVQAIES
EECCCCCEEHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCC
PDRQLVNELLRLDRYVDMLIPRGGASLHKLCREQSTIPVITGGIGVCHTFVDENADFEKA
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCHHHE
LLVIENAKIQRPSACNSLETLLVHQAVAKTFLPLLSARMHAFGVTLHASPLAMPYLADGK
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEECCCC
AKVVAVEAADYDDEWLSLDLNVDIVTDIDAAIDHIREHGTSHSDAILTRSLSHAEYFVRA
EEEEEEEECCCCCCEEEEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLDALTTYKWIGYGDDLVRS
CCCCEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCCHHHHHEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA