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The map label for this gene is proB [H]

Identifier: 170025505

GI number: 170025505

Start: 3598547

End: 3599650

Strand: Reverse

Name: proB [H]

Synonym: YPK_3288

Alternate gene names: 170025505

Gene position: 3599650-3598547 (Counterclockwise)

Preceding gene: 170025506

Following gene: 170025504

Centisome position: 76.76

GC content: 52.54

Gene sequence:

>1104_bases
ATGAGTGGCAGCCAGACATTGGTTGTGAAATTGGGGACGAGTGTACTGACCGGGGGTTCCCGCCGTCTTAACCGTGCCCA
TATTGTTGAATTGGTGCGTCAATGTGCCCAGCAACACGCAAAAGGTCATCGTATTGTTATTGTCACCTCTGGGGCGATTG
CTGCGGGTCGTGAACATTTAGGGTATCCAGAGTTGCCCGCCACTATTGCCTCGAAACAATTGCTAGCCGCGGTTGGGCAA
AGCCGGTTAATTCAGCTCTGGGAACAACTGTTTTCAATTTATGGCATTCATATCGGCCAGATGCTGCTAACCCGCGCAGA
TTTAGAGGATCGTGAGCGTTTTCTTAATGCCCGCGATACCATGAATGCGTTGCTTGATAACCGCATTGTACCGGTGATCA
ATGAGAACGACGCCGTCGCTACTGCTGAAATCAAAGTGGGCGATAACGATAATCTCTCCGCATTGGCGGCCATTTTGGCC
AGTGCAGATAAGCTACTGTTGTTGACCGATCAGGCGGGGTTGTACACCGCTGATCCGCGTAACAATCCAGAAGCGGAGTT
AATTCGCGAAGTGCATGGCATTGATGATGTCCTGCGCGGGATGGCCGGAGACAGCGTTTCGGGGCTCGGGACGGGGGGCA
TGGCGACTAAACTGCAAGCGGCGGATGTCGCGTGCCGTGCGGGTATTGATGTGGTGATTGCCGCAGGTAGCCAGGTGGGT
GTGATTGCCGATGTGATTGACGGTACGCCAGTCGGTACCCGTTTCCACTCATTGGAAACACCATTAGAAAACCGTAAACG
TTGGATTTTTGGTGCGCCACCGGCAGGTGAAATCACAGTGGACGACGGTGCTGTATTTGCGATCATGGAACGGGGCAGTT
CGCTGCTGCCAAAAGGCATTCGCAGCGTTAAAGGGGACTTCTCCCGTGGTGAAGTGATCCGTATCCGTAATCTCAATGGG
CGGGATTTAGCACACGGTGTTTCACGCTATAACAGTGATGCATTGCGCATGTTAGCGGGGCATCACTCGCAACAAATCAG
TGAAATCCTCGGATATGAATACGGCCCGGTGGCTGTTCATCGTGATGATATGATTGTCAGCTAA

Upstream 100 bases:

>100_bases
AAGGGGGTGATTTCGTATCAAGCCTGTTGGCATAATGCCTTGCGCCTGCTACAAAGACCTCTAGACCATTTCTTTTATTC
ATCCCAACGGTAGAAAAATT

Downstream 100 bases:

>100_bases
GGAGCAAACATGAACCTGTTAGAACATATGGGAAAAGCGGCCAAACAGGCTTCCTGGCAATTGGCGATGTTGAGTACGGC
CAAAAAAAATCAGGCACTGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSGSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQQHAKGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHIGQMLLTRADLEDRERFLNARDTMNALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILA
SADKLLLLTDQAGLYTADPRNNPEAELIREVHGIDDVLRGMAGDSVSGLGTGGMATKLQAADVACRAGIDVVIAAGSQVG
VIADVIDGTPVGTRFHSLETPLENRKRWIFGAPPAGEITVDDGAVFAIMERGSSLLPKGIRSVKGDFSRGEVIRIRNLNG
RDLAHGVSRYNSDALRMLAGHHSQQISEILGYEYGPVAVHRDDMIVS

Sequences:

>Translated_367_residues
MSGSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQQHAKGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHIGQMLLTRADLEDRERFLNARDTMNALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILA
SADKLLLLTDQAGLYTADPRNNPEAELIREVHGIDDVLRGMAGDSVSGLGTGGMATKLQAADVACRAGIDVVIAAGSQVG
VIADVIDGTPVGTRFHSLETPLENRKRWIFGAPPAGEITVDDGAVFAIMERGSSLLPKGIRSVKGDFSRGEVIRIRNLNG
RDLAHGVSRYNSDALRMLAGHHSQQISEILGYEYGPVAVHRDDMIVS
>Mature_366_residues
SGSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQQHAKGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQS
RLIQLWEQLFSIYGIHIGQMLLTRADLEDRERFLNARDTMNALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILAS
ADKLLLLTDQAGLYTADPRNNPEAELIREVHGIDDVLRGMAGDSVSGLGTGGMATKLQAADVACRAGIDVVIAAGSQVGV
IADVIDGTPVGTRFHSLETPLENRKRWIFGAPPAGEITVDDGAVFAIMERGSSLLPKGIRSVKGDFSRGEVIRIRNLNGR
DLAHGVSRYNSDALRMLAGHHSQQISEILGYEYGPVAVHRDDMIVS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=32.2916666666667, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=32.0689655172414, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=87.1232876712329, Blast_Score=647, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=33.0882352941176, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=33.0882352941176, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=34.3675417661098, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=33.8129496402878, Blast_Score=209, Evalue=5e-55,
Organism=Drosophila melanogaster, GI21357643, Length=282, Percent_Identity=34.3971631205674, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39291; Mature: 39160

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQQHAKGHRIVIVTSGAIAAGREHL
CCCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHIGQMLLTRADLEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MNALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILASADKLLLLTDQAGLYTADPR
HHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCEEECCCC
NNPEAELIREVHGIDDVLRGMAGDSVSGLGTGGMATKLQAADVACRAGIDVVIAAGSQVG
CCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
VIADVIDGTPVGTRFHSLETPLENRKRWIFGAPPAGEITVDDGAVFAIMERGSSLLPKGI
EEEECCCCCCCCCCHHHHCCCHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCCHHHH
RSVKGDFSRGEVIRIRNLNGRDLAHGVSRYNSDALRMLAGHHSQQISEILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCCCCEEEE
RDDMIVS
CCCEECC
>Mature Secondary Structure 
SGSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQQHAKGHRIVIVTSGAIAAGREHL
CCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHIGQMLLTRADLEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MNALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILASADKLLLLTDQAGLYTADPR
HHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCEEECCCC
NNPEAELIREVHGIDDVLRGMAGDSVSGLGTGGMATKLQAADVACRAGIDVVIAAGSQVG
CCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
VIADVIDGTPVGTRFHSLETPLENRKRWIFGAPPAGEITVDDGAVFAIMERGSSLLPKGI
EEEECCCCCCCCCCHHHHCCCHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCCHHHH
RSVKGDFSRGEVIRIRNLNGRDLAHGVSRYNSDALRMLAGHHSQQISEILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCCCCEEEE
RDDMIVS
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1851803; 1316937 [H]