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The map label for this gene is phoB [H]

Identifier: 170025493

GI number: 170025493

Start: 3583652

End: 3584341

Strand: Reverse

Name: phoB [H]

Synonym: YPK_3276

Alternate gene names: 170025493

Gene position: 3584341-3583652 (Counterclockwise)

Preceding gene: 170025496

Following gene: 170025492

Centisome position: 76.43

GC content: 48.55

Gene sequence:

>690_bases
ATGGCAAGACGCATACTGGTGGTGGAAGATGAAGCACCAATCCGTGAGATGGTGTGCTTTGTGTTGGAGCAAAATGGCTA
CCAACCGTTAGAAGCCGAAGATTATGACAGCGCGGTAGCTCGCCTGTCGGAGCCTTTTCCTGATTTAGTGCTGTTAGACT
GGATGTTACCCGGCGGCTCGGGTATTCAGTTTATTAAACATATGAAACGTGAAGCTTTGACCCGGGATATTCCGGTGATG
ATGCTGACCGCCCGTGGCGAAGAAGAAGATCGTGTGCGCGGTTTGGAAGTGGGCGCAGATGATTATATTACCAAGCCTTT
TTCACCTAAAGAGCTGGTAGCGCGGATCAAAGCGGTGATGCGGCGGATATCGCCAATGGCCGTTGAAGAAGTGATTGAAA
TGCAAGGGTTGAGCCTTGACCCTTCATCTCACAGGGTAATGGCGAATGATCAGGCGCTAGATATGGGGCCGACCGAGTTT
AAATTATTACACTTCTTTATGACTCATCCCGAGCGTGTTTATAGCCGTGAACAGTTGCTTAATTATGTTTGGGGCACTAA
CGTTTATGTAGAAGATCGTACGGTTGATGTGCATATTCGTCGGTTACGCAAGGCGCTTGAAACTGATGGTCACGATAAAA
TGGTACAAACTGTCCGGGGAACTGGATACCGTTTCTCAACGCGCTACTGA

Upstream 100 bases:

>100_bases
GCTCACTGAAGCGTGATATTTTTCATAAATCTGTCACAAAACTGACGCATAATGGCCCCGAAATCGAATAGTGACCGCTA
ACATACTGGCAGGGTTGATG

Downstream 100 bases:

>100_bases
GTTTTCAGTGCCGGAGAATTCGTTGTGTTAGAACGCTTATCATGGAAAACGCTGGCTTTAGAGCTGGCTCTTTTTTGTTT
GCCTGCATTATTACTGGGGG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MARRILVVEDEAPIREMVCFVLEQNGYQPLEAEDYDSAVARLSEPFPDLVLLDWMLPGGSGIQFIKHMKREALTRDIPVM
MLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPSSHRVMANDQALDMGPTEF
KLLHFFMTHPERVYSREQLLNYVWGTNVYVEDRTVDVHIRRLRKALETDGHDKMVQTVRGTGYRFSTRY

Sequences:

>Translated_229_residues
MARRILVVEDEAPIREMVCFVLEQNGYQPLEAEDYDSAVARLSEPFPDLVLLDWMLPGGSGIQFIKHMKREALTRDIPVM
MLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPSSHRVMANDQALDMGPTEF
KLLHFFMTHPERVYSREQLLNYVWGTNVYVEDRTVDVHIRRLRKALETDGHDKMVQTVRGTGYRFSTRY
>Mature_228_residues
ARRILVVEDEAPIREMVCFVLEQNGYQPLEAEDYDSAVARLSEPFPDLVLLDWMLPGGSGIQFIKHMKREALTRDIPVMM
LTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPSSHRVMANDQALDMGPTEFK
LLHFFMTHPERVYSREQLLNYVWGTNVYVEDRTVDVHIRRLRKALETDGHDKMVQTVRGTGYRFSTRY

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=89.9563318777293, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=39.7321428571429, Blast_Score=166, Evalue=9e-43,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.5, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=34.0807174887892, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=36.6515837104072, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=35.8744394618834, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=35.4978354978355, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=33.1818181818182, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.5545851528384, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=33.6283185840708, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=32.2727272727273, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=31.304347826087, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=27.5423728813559, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=27.0491803278689, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=37.6146788990826, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26447; Mature: 26316

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRILVVEDEAPIREMVCFVLEQNGYQPLEAEDYDSAVARLSEPFPDLVLLDWMLPGGS
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCC
GIQFIKHMKREALTRDIPVMMLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPSSHRVMANDQALDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NYVWGTNVYVEDRTVDVHIRRLRKALETDGHDKMVQTVRGTGYRFSTRY
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
ARRILVVEDEAPIREMVCFVLEQNGYQPLEAEDYDSAVARLSEPFPDLVLLDWMLPGGS
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCC
GIQFIKHMKREALTRDIPVMMLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPSSHRVMANDQALDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NYVWGTNVYVEDRTVDVHIRRLRKALETDGHDKMVQTVRGTGYRFSTRY
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]