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The map label for this gene is mak [H]

Identifier: 170025496

GI number: 170025496

Start: 3589743

End: 3590657

Strand: Reverse

Name: mak [H]

Synonym: YPK_3279

Alternate gene names: 170025496

Gene position: 3590657-3589743 (Counterclockwise)

Preceding gene: 170025498

Following gene: 170025493

Centisome position: 76.57

GC content: 50.05

Gene sequence:

>915_bases
ATGCGCATTGGTATAGATCTGGGTGGTACCAAAATAGAGGTCATAGCATTGGCCAATGATGGGCAGGTGCTTTTCCGTAA
ACGGGTTGGTACTCCACGCCATGATTATCAAAAAACCATACAGGCTATTGCTGACTTAATCGCTGATGCCGAGCACGCGA
CTGGCGAGCAAGGCAGTATCGGGGTTGGTATCCCTGGCACACTTTCTCCTTTTACCGGCAAAGTGAAAAACTCAAACTCA
GTATGGTTGAATGGGCGGGAGTTAGATAAAGATCTCTCGGTGTTTTTGAACCGCCCGGTGCGGTTAGCCAATGATGCGAA
CTGTCTGGCCGTTTCTGAGGCAACAGACGGGGCGGGGGCAGGTAAAAAATGGGTGTTTGCTGCCGTTATCGGGACGGGGT
GTGGCTCGGGTATTGCCATTGATGGGGGTTCGCATGCTGGCGGTAATGGTATTGCCGGTGAATGGGGGCATAACCCGTTA
CCTTGGCAAGATGAAGAAGAGCGAGAATATGCACAAGAAGTGACGTGCTATTGTGGGAAAAAAGGCTGTATTGAGACTTT
TGTATCGGGTACGGGTTTTGCCACTGATTACTTCAGGTTGAGTGGAAAACCGCTAAAAGGCCATGAAATTATTGAGTTGG
TTGAGCAGGGTGATGTTATCGCAGAACAGGCAATGAACAATTATGAGCGGCGTTTTGCCAAATCGCTGGCCCATGTCATT
AACCTGTTCGACCCCGATGTTGTGGTACTGGGGGGAGGGATGAGTAATGTTGACCGCTTGTATGACACATTACCTGCGTT
AATCAGCCCCTGGGTATTTGGCGGTGAGTGTGAAACGCCTATTCTTAAAGCCTTGCATGGTGATTCGAGTGGGGTCAGGG
GGGCTGCCTGGTTATGGAATACCCTTCGTCCTTGA

Upstream 100 bases:

>100_bases
TATTTTCATTATCATAGTTATCCAGATCACAGTTATCCTGCCTAGCAGGGTATTTTGATCGCGAGAAAATAAAACAATCT
TGTCAACGAGGTGAGATGCG

Downstream 100 bases:

>100_bases
CGCTACAGCGGTGTTAGCTACATTCACTCACCCGAATCACTTACTGGTCGTTGACCTTACAATAAATAGACTCATCAGGA
TTAATGAGCCTCATCCTTGA

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRIGIDLGGTKIEVIALANDGQVLFRKRVGTPRHDYQKTIQAIADLIADAEHATGEQGSIGVGIPGTLSPFTGKVKNSNS
VWLNGRELDKDLSVFLNRPVRLANDANCLAVSEATDGAGAGKKWVFAAVIGTGCGSGIAIDGGSHAGGNGIAGEWGHNPL
PWQDEEEREYAQEVTCYCGKKGCIETFVSGTGFATDYFRLSGKPLKGHEIIELVEQGDVIAEQAMNNYERRFAKSLAHVI
NLFDPDVVVLGGGMSNVDRLYDTLPALISPWVFGGECETPILKALHGDSSGVRGAAWLWNTLRP

Sequences:

>Translated_304_residues
MRIGIDLGGTKIEVIALANDGQVLFRKRVGTPRHDYQKTIQAIADLIADAEHATGEQGSIGVGIPGTLSPFTGKVKNSNS
VWLNGRELDKDLSVFLNRPVRLANDANCLAVSEATDGAGAGKKWVFAAVIGTGCGSGIAIDGGSHAGGNGIAGEWGHNPL
PWQDEEEREYAQEVTCYCGKKGCIETFVSGTGFATDYFRLSGKPLKGHEIIELVEQGDVIAEQAMNNYERRFAKSLAHVI
NLFDPDVVVLGGGMSNVDRLYDTLPALISPWVFGGECETPILKALHGDSSGVRGAAWLWNTLRP
>Mature_304_residues
MRIGIDLGGTKIEVIALANDGQVLFRKRVGTPRHDYQKTIQAIADLIADAEHATGEQGSIGVGIPGTLSPFTGKVKNSNS
VWLNGRELDKDLSVFLNRPVRLANDANCLAVSEATDGAGAGKKWVFAAVIGTGCGSGIAIDGGSHAGGNGIAGEWGHNPL
PWQDEEEREYAQEVTCYCGKKGCIETFVSGTGFATDYFRLSGKPLKGHEIIELVEQGDVIAEQAMNNYERRFAKSLAHVI
NLFDPDVVVLGGGMSNVDRLYDTLPALISPWVFGGECETPILKALHGDSSGVRGAAWLWNTLRP

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=299, Percent_Identity=69.2307692307692, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787363, Length=306, Percent_Identity=38.562091503268, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1790522, Length=305, Percent_Identity=27.2131147540984, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786891, Length=280, Percent_Identity=25.3571428571429, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787878, Length=211, Percent_Identity=26.5402843601896, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32464; Mature: 32464

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKIEVIALANDGQVLFRKRVGTPRHDYQKTIQAIADLIADAEHATGEQGSI
CEEEEECCCCEEEEEEEECCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
GVGIPGTLSPFTGKVKNSNSVWLNGRELDKDLSVFLNRPVRLANDANCLAVSEATDGAGA
EECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCC
GKKWVFAAVIGTGCGSGIAIDGGSHAGGNGIAGEWGHNPLPWQDEEEREYAQEVTCYCGK
CCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGCIETFVSGTGFATDYFRLSGKPLKGHEIIELVEQGDVIAEQAMNNYERRFAKSLAHVI
CHHHHHHHCCCCCEEHEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NLFDPDVVVLGGGMSNVDRLYDTLPALISPWVFGGECETPILKALHGDSSGVRGAAWLWN
HHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHH
TLRP
CCCC
>Mature Secondary Structure
MRIGIDLGGTKIEVIALANDGQVLFRKRVGTPRHDYQKTIQAIADLIADAEHATGEQGSI
CEEEEECCCCEEEEEEEECCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
GVGIPGTLSPFTGKVKNSNSVWLNGRELDKDLSVFLNRPVRLANDANCLAVSEATDGAGA
EECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCC
GKKWVFAAVIGTGCGSGIAIDGGSHAGGNGIAGEWGHNPLPWQDEEEREYAQEVTCYCGK
CCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGCIETFVSGTGFATDYFRLSGKPLKGHEIIELVEQGDVIAEQAMNNYERRFAKSLAHVI
CHHHHHHHCCCCCEEHEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NLFDPDVVVLGGGMSNVDRLYDTLPALISPWVFGGECETPILKALHGDSSGVRGAAWLWN
HHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHH
TLRP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]