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The map label for this gene is ruvB [H]

Identifier: 170024377

GI number: 170024377

Start: 2374118

End: 2375122

Strand: Reverse

Name: ruvB [H]

Synonym: YPK_2143

Alternate gene names: 170024377

Gene position: 2375122-2374118 (Counterclockwise)

Preceding gene: 170024378

Following gene: 170024374

Centisome position: 50.65

GC content: 50.25

Gene sequence:

>1005_bases
ATGATTGAAGCAGACCGTCTAATATCGGCCGCAGTTATCAATGATGAAGAGAGCATTGACCGGGCCATCCGCCCTAAGCT
ACTGACTGAATATGTTGGGCAACCTCATGTCCGTGAACAGATGGAAATCTTCATTCAGGCAGCAAAACAACGCGGTGATG
CACTCGATCACGTGCTGATATTTGGCCCTCCGGGTTTGGGTAAAACCACATTGGCGAACATTATTGCCAACGAGATGGGG
GTCAATCTGCGCACAACGTCCGGTCCGGTATTGGAAAAGGCCGGTGATTTAGCCGCCATGTTGACCAACCTGGAGCCGCA
TGATGTGCTATTTATTGATGAGATCCACCGTTTGTCACCGGTAGTAGAAGAGATTCTTTACCCGGCAATGGAAGATTATC
AATTGGACATCATGATTGGCGAAGGCCCCGCGGCTCGCTCAATCAAACTTGATTTACCGCCGTTTACCCTGATTGGTGCG
ACCACCCGAGCGGGTTCACTAACATCACCTTTGCGTGATCGGTTTGGTATCGTGCAGCGGCTGGAGTTTTATCAGGTGGC
TGATCTAGAGCATATTGTTTCACGCAGTGCTAAGTGCTTGGGGCTGGAACTCACGCCGGAGGGGGCACACCAGTTGGCAC
GTCGCTCAAGAGGAACGCCGCGTATTACCAACCGCCTATTGCGCCGTGTACGTGATTTCGCCGAAGTTAGAGCCGATGGT
GCCATTAACGGTGAGGTCGCAATGAAAGCGCTGGATATGCTTAATGTCGATGCTGAAGGGTTCGATTTTATGGACCGTAA
ATTGTTGTTAGCGGTTATCGATAAATTTATGGGCGGGCCAGTAGGGCTGGATAATCTGGCGGCAGCGATTGGTGAAGAGC
GGGAAACCATCGAAGATGTTCTGGAGCCGTATCTGATTCAACAAGGCTTTATTCAGCGTACTCCTCGTGGGCGAATTGCC
ACAAATCACGCGTATAAACATTTTGGTATTACTCGGGAAGAATAA

Upstream 100 bases:

>100_bases
GGCAAACGAATCCCGATGAATTTAAAAAAATTAGTCATTTGGGGGAGGGGCGTAGTTAGCCTCTGCGGCTTCAAATTCAA
AGGGTATGAAGGGGTAAGGG

Downstream 100 bases:

>100_bases
TACGTTTCACGCTGTGGGGGTTATAGGCCTAAACCCCACAGCGTGAAGTGATGACTATCCTCGTGCTTTTTGCGACAGGC
TCAGCACAAACATGGCGGCA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MIEADRLISAAVINDEESIDRAIRPKLLTEYVGQPHVREQMEIFIQAAKQRGDALDHVLIFGPPGLGKTTLANIIANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVADLEHIVSRSAKCLGLELTPEGAHQLARRSRGTPRITNRLLRRVRDFAEVRADG
AINGEVAMKALDMLNVDAEGFDFMDRKLLLAVIDKFMGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFIQRTPRGRIA
TNHAYKHFGITREE

Sequences:

>Translated_334_residues
MIEADRLISAAVINDEESIDRAIRPKLLTEYVGQPHVREQMEIFIQAAKQRGDALDHVLIFGPPGLGKTTLANIIANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVADLEHIVSRSAKCLGLELTPEGAHQLARRSRGTPRITNRLLRRVRDFAEVRADG
AINGEVAMKALDMLNVDAEGFDFMDRKLLLAVIDKFMGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFIQRTPRGRIA
TNHAYKHFGITREE
>Mature_334_residues
MIEADRLISAAVINDEESIDRAIRPKLLTEYVGQPHVREQMEIFIQAAKQRGDALDHVLIFGPPGLGKTTLANIIANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVADLEHIVSRSAKCLGLELTPEGAHQLARRSRGTPRITNRLLRRVRDFAEVRADG
AINGEVAMKALDMLNVDAEGFDFMDRKLLLAVIDKFMGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFIQRTPRGRIA
TNHAYKHFGITREE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=334, Percent_Identity=84.7305389221557, Blast_Score=576, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37050; Mature: 37050

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRLISAAVINDEESIDRAIRPKLLTEYVGQPHVREQMEIFIQAAKQRGDALDHVLI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
FGPPGLGKTTLANIIANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
VVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGATTRAGSLTSPLRDRFGIVQR
HHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LEFYQVADLEHIVSRSAKCLGLELTPEGAHQLARRSRGTPRITNRLLRRVRDFAEVRADG
HHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
AINGEVAMKALDMLNVDAEGFDFMDRKLLLAVIDKFMGGPVGLDNLAAAIGEERETIEDV
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHH
LEPYLIQQGFIQRTPRGRIATNHAYKHFGITREE
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MIEADRLISAAVINDEESIDRAIRPKLLTEYVGQPHVREQMEIFIQAAKQRGDALDHVLI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
FGPPGLGKTTLANIIANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
VVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGATTRAGSLTSPLRDRFGIVQR
HHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LEFYQVADLEHIVSRSAKCLGLELTPEGAHQLARRSRGTPRITNRLLRRVRDFAEVRADG
HHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
AINGEVAMKALDMLNVDAEGFDFMDRKLLLAVIDKFMGGPVGLDNLAAAIGEERETIEDV
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHH
LEPYLIQQGFIQRTPRGRIATNHAYKHFGITREE
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA