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The map label for this gene is cysU [H]

Identifier: 170023651

GI number: 170023651

Start: 1553519

End: 1554352

Strand: Direct

Name: cysU [H]

Synonym: YPK_1409

Alternate gene names: 170023651

Gene position: 1553519-1554352 (Clockwise)

Preceding gene: 170023650

Following gene: 170023652

Centisome position: 33.13

GC content: 53.96

Gene sequence:

>834_bases
ATGTTGTCGGCATCCAGTAAACGGGTTTTGCCCGGATTTACCCTCAGTCTTGGGAGTAGCCTGCTTTATACCTGCTTGAT
TTTGCTATTACCGCTTAGCGCGTTAGTGATGCAAGTTGCGCAGATGAGTTGGGCGCAGTACTGGGAGGTGATTACCAACC
CTCAAGTGGTTGCCGCGTATAAAGTGACGTTATTGGCCGCCGGCGTGGCCAGTGTGTTCAATGCGGTGTTTGGGATGTTG
ATGGCTTGGATTTTGACCCGCTATCAATTCCCCGGCCGCAGCATTCTGGATGGGTTAATGGATTTACCCTTCGCGTTACC
AACCGCAGTGGCTGGCCTGACGCTGGCCACACTGTTCTCCACCACGGGTTGGTACGGTGCTTGGTTAAATGAATTCGGTA
TCAAAGTCTCCTTTACTTGGTTGGGGATTGCGGTCGCCATGGCTTTCACCAGCATCCCGTTCGTGGTCCGCACGGTGCAA
CCGGTATTGGAAGAGTTGGGGCCAGAGTACGAAGAAGCCGCAGAAACTCTGGGAGCGAGCCGCTGGCAAAGCTTCCGTCT
GGTGGTGATGCCGGAATTGGCACCCGCGCTATTGGCCGGGACCGCATTATCCTTTACCCGCAGCTTGGGGGAGTTTGGTG
CGGTTATCTTTATCGCCGGTAATATTGCCTGGAAAACGGAAGTGACCTCGCTGATGATTTTCATCCGTTTGCAGGAATTT
GATTACCCGGCAGCCAGCGCCATTGCCTCGGTGATTCTGGCCGTGTCACTGGCGCTGTTGTTTAGCATTAACACGTTACA
AAGCCGCTTTGGTCGGCGGATAGGTGGGCACTAA

Upstream 100 bases:

>100_bases
TCCGAGTTGAAGATCAATTCGGTAGCTGGCCACAGGTGATGAGTACCCATTTTACTACTGGCGGCGTACTGGATAAATTG
TTAGCAGAAGGGCATCAGTA

Downstream 100 bases:

>100_bases
TGGTGGATGTTTCTGAATTTAACGGTGTTGCCCGCCCACCGATCAATTGGGGCAAATGGACGCTGATTGTCATTGGTGCG
GCGTTCTCGTTTTTGCTGCT

Product: sulfate/thiosulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLSASSKRVLPGFTLSLGSSLLYTCLILLLPLSALVMQVAQMSWAQYWEVITNPQVVAAYKVTLLAAGVASVFNAVFGML
MAWILTRYQFPGRSILDGLMDLPFALPTAVAGLTLATLFSTTGWYGAWLNEFGIKVSFTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGASRWQSFRLVVMPELAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
DYPAASAIASVILAVSLALLFSINTLQSRFGRRIGGH

Sequences:

>Translated_277_residues
MLSASSKRVLPGFTLSLGSSLLYTCLILLLPLSALVMQVAQMSWAQYWEVITNPQVVAAYKVTLLAAGVASVFNAVFGML
MAWILTRYQFPGRSILDGLMDLPFALPTAVAGLTLATLFSTTGWYGAWLNEFGIKVSFTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGASRWQSFRLVVMPELAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
DYPAASAIASVILAVSLALLFSINTLQSRFGRRIGGH
>Mature_277_residues
MLSASSKRVLPGFTLSLGSSLLYTCLILLLPLSALVMQVAQMSWAQYWEVITNPQVVAAYKVTLLAAGVASVFNAVFGML
MAWILTRYQFPGRSILDGLMDLPFALPTAVAGLTLATLFSTTGWYGAWLNEFGIKVSFTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGASRWQSFRLVVMPELAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
DYPAASAIASVILAVSLALLFSINTLQSRFGRRIGGH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=277, Percent_Identity=85.9205776173285, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI87082099, Length=259, Percent_Identity=36.2934362934363, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786980, Length=211, Percent_Identity=35.5450236966825, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787368, Length=214, Percent_Identity=28.5046728971963, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787369, Length=198, Percent_Identity=26.2626262626263, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30018; Mature: 30018

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSASSKRVLPGFTLSLGSSLLYTCLILLLPLSALVMQVAQMSWAQYWEVITNPQVVAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KVTLLAAGVASVFNAVFGMLMAWILTRYQFPGRSILDGLMDLPFALPTAVAGLTLATLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TTGWYGAWLNEFGIKVSFTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAETLGAS
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFRLVVMPELAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
HHHCEEEEEECHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILAVSLALLFSINTLQSRFGRRIGGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSASSKRVLPGFTLSLGSSLLYTCLILLLPLSALVMQVAQMSWAQYWEVITNPQVVAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KVTLLAAGVASVFNAVFGMLMAWILTRYQFPGRSILDGLMDLPFALPTAVAGLTLATLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TTGWYGAWLNEFGIKVSFTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAETLGAS
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFRLVVMPELAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
HHHCEEEEEECHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILAVSLALLFSINTLQSRFGRRIGGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]