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The map label for this gene is polC [H]

Identifier: 169627433

GI number: 169627433

Start: 329582

End: 330565

Strand: Direct

Name: polC [H]

Synonym: MAB_0329

Alternate gene names: 169627433

Gene position: 329582-330565 (Clockwise)

Preceding gene: 169627432

Following gene: 169627434

Centisome position: 6.5

GC content: 65.96

Gene sequence:

>984_bases
ATGCCGATCTGGGGGCAGACGCCCGACCACAGCCCCAAGTGGGTGGTGGTTGACCTGGAGACTTCAGGTTTCCAGCCGGG
GCGGGCGCGTGTCATCAGCGTTGCGGCATTGGCGATGGATGCCGACGGGACCATCACCGACAGCGTGGTGAGTCTGCTCA
ACCCCGGTGTCGATCCGGGCCCCACGCACGTCCACGGCCTCACCCCGGAGATGCTGGAGGGCCAGCCGGAGTTCTCCGAT
ATCGCGCCCGATCTGATCGCGCTGCTGCGCGGGCGGACGTTGGTCGCGCACAACGTCGGCTTCGACTACTCGTTCCTGGC
GGCCGAGGCCGAGCTGGTGGGAGCCGAACTGCCCACCGAGGCCGTCATGTGCACCGTCGAGCTGACGCGCCGTCTCGAAC
TGGACACACCCAACCTGCGCTTGGAAACCCTTGCCGCGCATTGGGGTGTCGAGCAGCTCAAGGCTCATGACGCTTATGAC
GATGCGCGGGTGCTGGCCGAGGTGCTGCCGAAGGTGCTGGAGCGCGCCCGCGAGCGTGGCACCTGGCTGCCCATCCGCGA
GACCACACGTAAGCGCTGGCCCAACGGCCGCGTTACGCACGATGAGCTGCGTCCACTCAAGACACTGGCGTCGCGCCAGC
CGTGCCACTGGCTCAACCCCGGCCCGTATACCGCGGGCCAGCCGCTCGTGCAGGGCATGCGTGTGGCATTGAGCTCGGAG
GTCTCGCGCACCCACGAAGAGCTGATCGAGCAGATCATGCAGGCCGGTCTCGCCTACGCCGATGCGGTCGACGAGGACAC
CTCGCTGGTGATCTGCAACCAGGAAAACCCAGAGCAGGGCAAGGGTTTCCATGCCCGCTCATTGGGCGTACCGCTGCTCG
ATGACCGGACGTTCATGACCGAGGTGCGCCGCGTTGTCGGTGGCACCGATATCGAACAATTCACGGTTCCCAGTCGTGCC
GGAGCTCAGTACTCGCTGTTTTGA

Upstream 100 bases:

>100_bases
ACGGTCCCACCGAGGTGCACAAGTACGCCATCGGACGCCAGGTGCTTCGGACTCCGCGCGCCTAGGGCGGGTTGTCGGTC
CCGCGTGCCATCCTTCAGTC

Downstream 100 bases:

>100_bases
TGAGTCCAGCCTGATGCGCCGAATCCTCGCTGTAGTGCTGACGATTGCGCTGCTGGTCAGCGCATGCGGGAAGCACTCGG
CGATCGTGGTGGCCGAGCTG

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPIWGQTPDHSPKWVVVDLETSGFQPGRARVISVAALAMDADGTITDSVVSLLNPGVDPGPTHVHGLTPEMLEGQPEFSD
IAPDLIALLRGRTLVAHNVGFDYSFLAAEAELVGAELPTEAVMCTVELTRRLELDTPNLRLETLAAHWGVEQLKAHDAYD
DARVLAEVLPKVLERARERGTWLPIRETTRKRWPNGRVTHDELRPLKTLASRQPCHWLNPGPYTAGQPLVQGMRVALSSE
VSRTHEELIEQIMQAGLAYADAVDEDTSLVICNQENPEQGKGFHARSLGVPLLDDRTFMTEVRRVVGGTDIEQFTVPSRA
GAQYSLF

Sequences:

>Translated_327_residues
MPIWGQTPDHSPKWVVVDLETSGFQPGRARVISVAALAMDADGTITDSVVSLLNPGVDPGPTHVHGLTPEMLEGQPEFSD
IAPDLIALLRGRTLVAHNVGFDYSFLAAEAELVGAELPTEAVMCTVELTRRLELDTPNLRLETLAAHWGVEQLKAHDAYD
DARVLAEVLPKVLERARERGTWLPIRETTRKRWPNGRVTHDELRPLKTLASRQPCHWLNPGPYTAGQPLVQGMRVALSSE
VSRTHEELIEQIMQAGLAYADAVDEDTSLVICNQENPEQGKGFHARSLGVPLLDDRTFMTEVRRVVGGTDIEQFTVPSRA
GAQYSLF
>Mature_326_residues
PIWGQTPDHSPKWVVVDLETSGFQPGRARVISVAALAMDADGTITDSVVSLLNPGVDPGPTHVHGLTPEMLEGQPEFSDI
APDLIALLRGRTLVAHNVGFDYSFLAAEAELVGAELPTEAVMCTVELTRRLELDTPNLRLETLAAHWGVEQLKAHDAYDD
ARVLAEVLPKVLERARERGTWLPIRETTRKRWPNGRVTHDELRPLKTLASRQPCHWLNPGPYTAGQPLVQGMRVALSSEV
SRTHEELIEQIMQAGLAYADAVDEDTSLVICNQENPEQGKGFHARSLGVPLLDDRTFMTEVRRVVGGTDIEQFTVPSRAG
AQYSLF

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1786409, Length=169, Percent_Identity=28.9940828402367, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR023223
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36030; Mature: 35899

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIWGQTPDHSPKWVVVDLETSGFQPGRARVISVAALAMDADGTITDSVVSLLNPGVDPG
CCCCCCCCCCCCCEEEEEEECCCCCCCHHHHEEHHHHEECCCCCHHHHHHHHHCCCCCCC
PTHVHGLTPEMLEGQPEFSDIAPDLIALLRGRTLVAHNVGFDYSFLAAEAELVGAELPTE
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
AVMCTVELTRRLELDTPNLRLETLAAHWGVEQLKAHDAYDDARVLAEVLPKVLERARERG
HHEEHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
TWLPIRETTRKRWPNGRVTHDELRPLKTLASRQPCHWLNPGPYTAGQPLVQGMRVALSSE
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VSRTHEELIEQIMQAGLAYADAVDEDTSLVICNQENPEQGKGFHARSLGVPLLDDRTFMT
HHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCCCCCCEEHHHCCCCCCCCHHHHH
EVRRVVGGTDIEQFTVPSRAGAQYSLF
HHHHHHCCCCCHHEECCCCCCCCCCCC
>Mature Secondary Structure 
PIWGQTPDHSPKWVVVDLETSGFQPGRARVISVAALAMDADGTITDSVVSLLNPGVDPG
CCCCCCCCCCCCEEEEEEECCCCCCCHHHHEEHHHHEECCCCCHHHHHHHHHCCCCCCC
PTHVHGLTPEMLEGQPEFSDIAPDLIALLRGRTLVAHNVGFDYSFLAAEAELVGAELPTE
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
AVMCTVELTRRLELDTPNLRLETLAAHWGVEQLKAHDAYDDARVLAEVLPKVLERARERG
HHEEHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
TWLPIRETTRKRWPNGRVTHDELRPLKTLASRQPCHWLNPGPYTAGQPLVQGMRVALSSE
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VSRTHEELIEQIMQAGLAYADAVDEDTSLVICNQENPEQGKGFHARSLGVPLLDDRTFMT
HHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCCCCCCEEHHHCCCCCCCCHHHHH
EVRRVVGGTDIEQFTVPSRAGAQYSLF
HHHHHHCCCCCHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]