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The map label for this gene is gcvH2 [H]

Identifier: 169824160

GI number: 169824160

Start: 520592

End: 520972

Strand: Direct

Name: gcvH2 [H]

Synonym: FMG_0463

Alternate gene names: 169824160

Gene position: 520592-520972 (Clockwise)

Preceding gene: 169824159

Following gene: 169824161

Centisome position: 28.96

GC content: 33.86

Gene sequence:

>381_bases
ATGGCAGAAGTAGCAAAAGATTTATTGTACACAAAAGATCATGAATGGATTAAAGTAGACGGAGATGTTTATGAAATTGG
TCTTGCAGATTATGCACAAGACAGTTTAGGAGACATCGTATACGTTGAATTACCAGATGTAGATGATGAAATTGATGCAG
AAGACTCTGTAGCAAGTGTTGAATCTGTAAAAGCAGCATCAGAAGTTTATACTCCATTTGCTTGTACTATAGTAGAAGTT
AATGAAGAATTAGATGATGAACCAGGCAATATCAACAAAGCTCCATATGAAAGCTGGATTGCTAAGGTTAAATTCGAAGA
CTTCGATGAATCTAAACTAATGAATGCTGATGAATACGAAAAATTCTTAGGAGAATTATAA

Upstream 100 bases:

>100_bases
AGCATTAGGTGATGAAGTAGATGTTGTTATCAGAAATAAACCAGCTAAAGCAACAGTTGTTAAAAGAAAATTTTTGGATA
GAAAATAGGGGGAAAAGAAA

Downstream 100 bases:

>100_bases
TGTATCCATACATCTCAGTTACTGATCAAGAAATTGAGGAAATGCTAAAAACAATTGGCATTTCCTCTCTAGATGAACTA
TATTCAGACGTTCCTGAAAA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MAEVAKDLLYTKDHEWIKVDGDVYEIGLADYAQDSLGDIVYVELPDVDDEIDAEDSVASVESVKAASEVYTPFACTIVEV
NEELDDEPGNINKAPYESWIAKVKFEDFDESKLMNADEYEKFLGEL

Sequences:

>Translated_126_residues
MAEVAKDLLYTKDHEWIKVDGDVYEIGLADYAQDSLGDIVYVELPDVDDEIDAEDSVASVESVKAASEVYTPFACTIVEV
NEELDDEPGNINKAPYESWIAKVKFEDFDESKLMNADEYEKFLGEL
>Mature_125_residues
AEVAKDLLYTKDHEWIKVDGDVYEIGLADYAQDSLGDIVYVELPDVDDEIDAEDSVASVESVKAASEVYTPFACTIVEVN
EELDDEPGNINKAPYESWIAKVKFEDFDESKLMNADEYEKFLGEL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=37.9310344827586, Blast_Score=103, Evalue=5e-23,
Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=37.0689655172414, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=42.8571428571429, Blast_Score=100, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=47.4137931034483, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=44.5378151260504, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=43.3333333333333, Blast_Score=97, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=40.650406504065, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14182; Mature: 14050

Theoretical pI: Translated: 3.63; Mature: 3.63

Prosite motif: PS00018 EF_HAND_1 ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVAKDLLYTKDHEWIKVDGDVYEIGLADYAQDSLGDIVYVELPDVDDEIDAEDSVASV
CHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHH
ESVKAASEVYTPFACTIVEVNEELDDEPGNINKAPYESWIAKVKFEDFDESKLMNADEYE
HHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCHHHHH
KFLGEL
HHHCCC
>Mature Secondary Structure 
AEVAKDLLYTKDHEWIKVDGDVYEIGLADYAQDSLGDIVYVELPDVDDEIDAEDSVASV
HHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHH
ESVKAASEVYTPFACTIVEVNEELDDEPGNINKAPYESWIAKVKFEDFDESKLMNADEYE
HHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCHHHHH
KFLGEL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]