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The map label for this gene is mnmG

Identifier: 167626210

GI number: 167626210

Start: 5215529

End: 5217418

Strand: Reverse

Name: mnmG

Synonym: Shal_4305

Alternate gene names: 167626210

Gene position: 5217418-5215529 (Counterclockwise)

Preceding gene: 167626211

Following gene: 167626209

Centisome position: 99.82

GC content: 44.44

Gene sequence:

>1890_bases
ATGCAATTTCATGAACGGTTTGATGTAATCGTTGTTGGTGGTGGTCATGCTGGTACAGAAGCCGCTTTAGCTTCTGCTAG
AATGGGCTCCAAAACACTTTTACTGACCCATAACATAGATACTTTAGGACAGATGTCCTGTAATCCTGCTATCGGCGGCA
TAGGTAAAGGACACTTAGTCAAAGAAATTGATGCATTAGGTGGAGCAATGGCTGTTGCTACCGATTTTGCAGGAATTCAA
TTTCGTACATTAAATTCAAGTAAGGGGCCAGCTGTTCGTGCAACTCGCGCACAAGCAGACAGAGCACTTTATCGACACAA
AATCCAAGAAATTTTACAGCACCAAACGAATCTACGTATTTTCCAACAAGCGGTAGACGATCTTGTTGTTGAAAATAACA
AAGTGATTGGTGTTGTCACACAGATGGGCTTGGCCTTTGAAGCTCCAGCAGTTGTCTTAACTGCTGGGACATTCCTTGGT
GGAAAGATCCATATCGGACTCGAAAACTACAGCGGTGGTCGAGCTGGTGATCCACCGTCAATAGCCTTGGCTCATCGACT
AAGAGAATTACCGATCCGTGTTGGACGTTTAAAAACGGGGACACCTCCACGTATCGATGCCAATACCATTGATTTCTCTC
TAATGACAGAGCAAAAAGGTGATAATCCATTACCAGTGATGTCATTTATCGGCGATGTTAACGACCACCCTGAACAAGTT
TCTTGTTACATCACTCATACCAATGAGCGTACTCACGATATTATTCGTGGTGGTTTAGACAGAAGTCCAATGTATTCAGG
GATCATCGAAGGTATTGGTCCGCGTTACTGCCCTTCAATCGAAGACAAGATCAATCGCTTTGCAGACAAAACGTCGCATC
AGATCTTTATTGAGCCAGAGGGATTAAATACGACTGAGATCTATCCAAACGGTATTTCGACCAGTTTGCCTTTTGACGTT
CAGTTGAACTTGGTTCGCTCAATCAAAGGTATGGAAAATGCTGAGATCATGCGTCCAGGTTATGCGATTGAGTATGATTA
CTTTGATCCTCGCGATCTTAAAAATTCATTAGAAACAAAAGCCATTAGTGGCTTGTTCTTTGCTGGACAGATTAACGGTA
CTACCGGCTACGAAGAAGCTGGCGCACAAGGTTTGTTAGCGGGCATGAATGCTTCGCTGCAGGTTCAAGGTAAGGAAGCT
TGGGCTCCACGTCGTGATCAAGCGTATCTAGGTGTATTGGTAGACGACTTATCGACGTTAGGTACCAAAGAACCGTACCG
CATGTTTACCAGCCGTGCGGAATACCGTTTGTTACTACGTGAAGATAACGCCGATCTACGTTTAACCGAGAAAGGCCGCG
AACTTGGCTTAGTTGACGATAAACGTTGGGCACTGTTTAGTGAGAAGATGGAATCGATCGAAACTGAACTGCAACGTTTA
CGTGGTCAGTGGATCCACCCTAACTCACCGTTAGTTGAGGCGCTTAATCCGAATTTGAATACGCCGATCACGCGTGAAGC
GACATTCGAAGATCTACTTCGTCGTCCAGAAATGGATTATCCAAAGCTAATGTCTATCGATGGCTTCGGTCCAGGACTTG
AAGATCAACGTGCTGCAGAGCAAGTACAGATCCAAGTTAAGTATTCTGGTTATATTCAACGCCAGCAAGGTGAAATTGAT
AAAGCGATTCGCCATGAAACCACTTTGCTACCGCTAGATCTGGATTATCAAGAAGTACCAGGTTTGTCGAATGAGGTGAT
TGCTAAGATGAATGAGCATAAGCCTGAGACGATTGGCCAAGCTTCACGTATCTCTGGAATGACTCCTGCTGCAATCTCAA
TCTTGTTGGTCCATCTGAAAAAACGAGGTTTATTGAGAAAAAGCGCCTAG

Upstream 100 bases:

>100_bases
GATCAAAACAAGCTAAATAGATCGTTCACCTTAAGCAAAGCAAATGCTAAAATGATCCGCTCAAAAACAATTTATCCTTT
TAAGAATCGAAGGCTGTCGA

Downstream 100 bases:

>100_bases
TTCGTTACAGATTCGCGAGCTTTGCATGATAAAGGGAAGCTTTGAGGCTTCCTTTCATCTTTTCCGCGATTCATAATACT
GATTGATTTTTGATTCGCTT

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAVATDFAGIQ
FRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQTNLRIFQQAVDDLVVENNKVIGVVTQMGLAFEAPAVVLTAGTFLG
GKIHIGLENYSGGRAGDPPSIALAHRLRELPIRVGRLKTGTPPRIDANTIDFSLMTEQKGDNPLPVMSFIGDVNDHPEQV
SCYITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKINRFADKTSHQIFIEPEGLNTTEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKAISGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WAPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDKRWALFSEKMESIETELQRL
RGQWIHPNSPLVEALNPNLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPGLEDQRAAEQVQIQVKYSGYIQRQQGEID
KAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQASRISGMTPAAISILLVHLKKRGLLRKSA

Sequences:

>Translated_629_residues
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAVATDFAGIQ
FRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQTNLRIFQQAVDDLVVENNKVIGVVTQMGLAFEAPAVVLTAGTFLG
GKIHIGLENYSGGRAGDPPSIALAHRLRELPIRVGRLKTGTPPRIDANTIDFSLMTEQKGDNPLPVMSFIGDVNDHPEQV
SCYITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKINRFADKTSHQIFIEPEGLNTTEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKAISGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WAPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDKRWALFSEKMESIETELQRL
RGQWIHPNSPLVEALNPNLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPGLEDQRAAEQVQIQVKYSGYIQRQQGEID
KAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQASRISGMTPAAISILLVHLKKRGLLRKSA
>Mature_629_residues
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAVATDFAGIQ
FRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQTNLRIFQQAVDDLVVENNKVIGVVTQMGLAFEAPAVVLTAGTFLG
GKIHIGLENYSGGRAGDPPSIALAHRLRELPIRVGRLKTGTPPRIDANTIDFSLMTEQKGDNPLPVMSFIGDVNDHPEQV
SCYITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKINRFADKTSHQIFIEPEGLNTTEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKAISGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WAPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDKRWALFSEKMESIETELQRL
RGQWIHPNSPLVEALNPNLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPGLEDQRAAEQVQIQVKYSGYIQRQQGEID
KAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQASRISGMTPAAISILLVHLKKRGLLRKSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=632, Percent_Identity=46.0443037974684, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI19882217, Length=657, Percent_Identity=44.2922374429224, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI183227703, Length=672, Percent_Identity=43.3035714285714, Blast_Score=481, Evalue=1e-136,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=73.6089030206677, Blast_Score=976, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=639, Percent_Identity=40.6885758998435, Blast_Score=470, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=626, Percent_Identity=46.3258785942492, Blast_Score=502, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24658174, Length=638, Percent_Identity=45.2978056426332, Blast_Score=505, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_SHEHH (B0TQG5)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001676504.1
- ProteinModelPortal:   B0TQG5
- SMR:   B0TQG5
- GeneID:   5905501
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_4305
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   SHAL458817:SHAL_4305-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69455; Mature: 69455

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 125-125 BINDING 180-180 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAVATDFAGIQFRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQTNLRI
HHHHHHCCHHEEHCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQQAVDDLVVENNKVIGVVTQMGLAFEAPAVVLTAGTFLGGKIHIGLENYSGGRAGDPPS
HHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECEEECCEEEEEEECCCCCCCCCCCH
IALAHRLRELPIRVGRLKTGTPPRIDANTIDFSLMTEQKGDNPLPVMSFIGDVNDHPEQV
HHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCEE
SCYITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKINRFADKTSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLNTTEIYPNGISTSLPFDVQLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETK
CCCCCEECCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
AISGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEAWAPRRDQAYLGVLVDDLSTL
HHCCEEEEEEECCCCCCHHCCCCEEEECCCCEEEECCHHCCCCCCCCCEEHHHHHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDKRWALFSEKMESIETELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHH
RGQWIHPNSPLVEALNPNLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPGLEDQRAAE
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCC
QVQIQVKYSGYIQRQQGEIDKAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQ
EEEEEEEECCEEEECCCHHHHHHHCCCEEEEECCCHHHCCCCCHHHHHHHHCCCCHHHCC
ASRISGMTPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAVATDFAGIQFRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQTNLRI
HHHHHHCCHHEEHCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQQAVDDLVVENNKVIGVVTQMGLAFEAPAVVLTAGTFLGGKIHIGLENYSGGRAGDPPS
HHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECEEECCEEEEEEECCCCCCCCCCCH
IALAHRLRELPIRVGRLKTGTPPRIDANTIDFSLMTEQKGDNPLPVMSFIGDVNDHPEQV
HHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCEE
SCYITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKINRFADKTSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLNTTEIYPNGISTSLPFDVQLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETK
CCCCCEECCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
AISGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEAWAPRRDQAYLGVLVDDLSTL
HHCCEEEEEEECCCCCCHHCCCCEEEECCCCEEEECCHHCCCCCCCCCEEHHHHHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDKRWALFSEKMESIETELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHH
RGQWIHPNSPLVEALNPNLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPGLEDQRAAE
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCC
QVQIQVKYSGYIQRQQGEIDKAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQ
EEEEEEEECCEEEECCCHHHHHHHCCCEEEEECCCHHHCCCCCHHHHHHHHCCCCHHHCC
ASRISGMTPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA