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The map label for this gene is gidB

Identifier: 167626209

GI number: 167626209

Start: 5214786

End: 5215409

Strand: Reverse

Name: gidB

Synonym: Shal_4304

Alternate gene names: 167626209

Gene position: 5215409-5214786 (Counterclockwise)

Preceding gene: 167626210

Following gene: 167626208

Centisome position: 99.78

GC content: 45.03

Gene sequence:

>624_bases
GTGTTATCTGCCCAATTAAATGATTATTTGGCCGAAGTTGGCCTGACTGCTACCGAGCAGCAAAAGAAGCAGCTTGTAGA
CTTTGTCGGCATGCTTAACAAGTGGAATAAGGCATTTAACCTGACATCTGTACGTGATCCTGAGCAGATGTTGATCCGTC
ATATTATGGACAGTTTAGTGGTGTCGCCTCACCTTAAGGGATCGCGTTTTATAGACGTAGGCACCGGCCCTGGTTTACCT
GGGATCCCGCTGGCGATCTTAAATCCTGACAAAGAATTTGTACTGCTCGATAGTTTAGGTAAGCGGATCCGTTTTCAAAA
GCAGGTGCAGTTTGAGTTAGGTATCAATAATATTTCTTCAATAGAAAGCCGTGTTGAAGCTTACCAACCTGAAGAACTAT
TCGATGGTGTGTTAAGCCGTGCGTTCGCTTCTATTCAAGATATGCTGCACTGGTGCCACCACCTCCCCAAAGCTGATGGT
TGCTTCTATGCGCTCAAGGGGCAATTGAGTGAAGATGAGATGGCACAACTGCCTGCGGGTTTTGTGGTAACAGATATCTT
TGAGCTTAAAGTGCCTAAATTGGATGAACAGCGTCATCTGTTACGTGTCATTAAGCAAGATTGA

Upstream 100 bases:

>100_bases
CTTTGCATGATAAAGGGAAGCTTTGAGGCTTCCTTTCATCTTTTCCGCGATTCATAATACTGATTGATTTTTGATTCGCT
TTATTTTGAGGACACAAACT

Downstream 100 bases:

>100_bases
TTAATTTTTAATGCGGTTGTTACTTTTTGATGACCGTAGCTTATTTTATCTCTGTCGCGATAAAAATAGACTAGGTGAAG
CCAGTGGTTGCAGACAAAGC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLVVSPHLKGSRFIDVGTGPGLP
GIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISSIESRVEAYQPEELFDGVLSRAFASIQDMLHWCHHLPKADG
CFYALKGQLSEDEMAQLPAGFVVTDIFELKVPKLDEQRHLLRVIKQD

Sequences:

>Translated_207_residues
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLVVSPHLKGSRFIDVGTGPGLP
GIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISSIESRVEAYQPEELFDGVLSRAFASIQDMLHWCHHLPKADG
CFYALKGQLSEDEMAQLPAGFVVTDIFELKVPKLDEQRHLLRVIKQD
>Mature_207_residues
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLVVSPHLKGSRFIDVGTGPGLP
GIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISSIESRVEAYQPEELFDGVLSRAFASIQDMLHWCHHLPKADG
CFYALKGQLSEDEMAQLPAGFVVTDIFELKVPKLDEQRHLLRVIKQD

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=203, Percent_Identity=59.6059113300493, Blast_Score=260, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_SHEHH (B0TQG4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001676503.1
- ProteinModelPortal:   B0TQG4
- SMR:   B0TQG4
- GeneID:   5905500
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_4304
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   SHAL458817:SHAL_4304-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23399; Mature: 23399

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLV
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSPHLKGSRFIDVGTGPGLPGIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISS
CCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHH
IESRVEAYQPEELFDGVLSRAFASIQDMLHWCHHLPKADGCFYALKGQLSEDEMAQLPAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCC
FVVTDIFELKVPKLDEQRHLLRVIKQD
HHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLV
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSPHLKGSRFIDVGTGPGLPGIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISS
CCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHH
IESRVEAYQPEELFDGVLSRAFASIQDMLHWCHHLPKADGCFYALKGQLSEDEMAQLPAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCC
FVVTDIFELKVPKLDEQRHLLRVIKQD
HHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA