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The map label for this gene is ada [H]

Identifier: 167623472

GI number: 167623472

Start: 1862328

End: 1863830

Strand: Direct

Name: ada [H]

Synonym: Shal_1541

Alternate gene names: 167623472

Gene position: 1862328-1863830 (Clockwise)

Preceding gene: 167623470

Following gene: 167623473

Centisome position: 35.63

GC content: 46.11

Gene sequence:

>1503_bases
ATGGACTGTGGAATGCTAAATCACGACTGTAAGATTGAAAGAGAAGCATGCCGCAAGGCTAGATTAGCTCGAGATCCCAG
GTTTGATGGACAGTTTTTTATTGGGGTATTTACCACCAAAATTTACTGTAGGCCAATTTGCCCTGCGGTGAGTCCAAAAG
AAGAAAATGTGCGGTATTTTGATTCAGCTCTTCAAGCTGCAAATGCTGGCTTAAGGCCTTGTCTACGTTGCCGGCCAGAT
AGCGCTCCACTCTCTTATGCATGGAAGGGGACACAAACTACCTTAGAGCGGGCAAAGGTCATAATCGATGCTGGAGAACT
TTCTGGGGATGATGGGCTGAGCGTAGAGGCTCTGTCTGAGCGGTTAGGGATTAGCAGCCGTTACCTCAGAAAACTTTTTA
GCGAGAAAATGGGGGTCTCGGTTAAAGGTTATGTGCAATACCGACAACTCATGTTTGCAAAGCAATTATTGCATCAAACC
CAGCTGAGTATCACTCAAGTCGCATTTGCGGCGGGCTTTAACAGTATTCGACGCTTTAATGAAGTCTTTAAGCAAGCATT
GCAATTAACTCCCTCAGCGCTGAGAAAAGCGTCCACAAAAAGGCCACCATCTGACGAGTTGCAACAACAAGGACAAATTA
CATTGCGATTACAGCTTAGCTATCGTCCGCCACTTGCCTGGACGCAACAGCATCGCTTTTATGAGGTTCGTGCCGTAGAG
CAAATGGAGTGGCTTGAAGATCCACTTAGTTATGGTCGAAGTTTCACCTTAAATGGAGTTAGTGGTTACTTCAATGCCAA
GCTAGATGCAAAGGCGAATGTGTTCAATGTCGATATTGTGTTAGATAATGAGTCAGGCCTTTCCCAGCTTAGTTTCATTA
TTAGCAGCATTCGTAGGGTATTGGACTTAGATGCCAACGTGCTTGAGATCGACAGTGTGCTTGCATCGCTAGACCCTTTA
GGAAAACAGATGATCCCAGGGTTACGTTTACCTGGGGTATGGTCTGCATTTGAAGCAGGGTGCCGAGCTATATTAGGGCA
ACAGGTCAGTATTATACAGGCCTCTAAGCTACTGAATCAGTTAGTACTTGCCTACGGGGAGCAGCGCTTGATTGGCGCAA
AGCAAGTGACGCTATTTCCATTACCTGAGACGATAGCGAAAGCCGAATTAAACGAGCTAAAAATGCCTGGAGTCAGGAAG
AGTGCGCTCAATGCCCTAGGTCAGTTTGTCAGTGATAACCCAGAAGCTGAGGTTGAGCAATGGCTATCGATAAAAGGTAT
TGGACCATGGACTACCGCCTACGCCAAAATGCGTGGCTTGAGTTATCCCGATGTTTTACTGTGCGGCGATCTTGTGGTTA
AAAAACGCGTGCTAAGTCTTTATGCCGCAAAGGCGGATAAGTTAGCAACATCAAATGGCTATGATGAGCTAACTCAAGAG
CTTGCCAAACACGTTGCTCCATGGGGCAGTTATCTCACATTTCAGCTTTGGAACCTTTCATGA

Upstream 100 bases:

>100_bases
AAATAAACCCGAGGTATTTATTTCCAACTTATATTCAGTTCCGTTTTCCGCTAGTGATGAGCAGGCAGGTTGCGTAAGAT
TATAGTCATAATGAGTTTTT

Downstream 100 bases:

>100_bases
ATAAACCGTTACTGCCATTAGCTAAATTGAATTTTAAATCCGAGTTCGGCGCGCTCTCTGTGTGCGCTAATGCAGAGGGC
ATCACTCACGTTAACTTTAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MDCGMLNHDCKIEREACRKARLARDPRFDGQFFIGVFTTKIYCRPICPAVSPKEENVRYFDSALQAANAGLRPCLRCRPD
SAPLSYAWKGTQTTLERAKVIIDAGELSGDDGLSVEALSERLGISSRYLRKLFSEKMGVSVKGYVQYRQLMFAKQLLHQT
QLSITQVAFAAGFNSIRRFNEVFKQALQLTPSALRKASTKRPPSDELQQQGQITLRLQLSYRPPLAWTQQHRFYEVRAVE
QMEWLEDPLSYGRSFTLNGVSGYFNAKLDAKANVFNVDIVLDNESGLSQLSFIISSIRRVLDLDANVLEIDSVLASLDPL
GKQMIPGLRLPGVWSAFEAGCRAILGQQVSIIQASKLLNQLVLAYGEQRLIGAKQVTLFPLPETIAKAELNELKMPGVRK
SALNALGQFVSDNPEAEVEQWLSIKGIGPWTTAYAKMRGLSYPDVLLCGDLVVKKRVLSLYAAKADKLATSNGYDELTQE
LAKHVAPWGSYLTFQLWNLS

Sequences:

>Translated_500_residues
MDCGMLNHDCKIEREACRKARLARDPRFDGQFFIGVFTTKIYCRPICPAVSPKEENVRYFDSALQAANAGLRPCLRCRPD
SAPLSYAWKGTQTTLERAKVIIDAGELSGDDGLSVEALSERLGISSRYLRKLFSEKMGVSVKGYVQYRQLMFAKQLLHQT
QLSITQVAFAAGFNSIRRFNEVFKQALQLTPSALRKASTKRPPSDELQQQGQITLRLQLSYRPPLAWTQQHRFYEVRAVE
QMEWLEDPLSYGRSFTLNGVSGYFNAKLDAKANVFNVDIVLDNESGLSQLSFIISSIRRVLDLDANVLEIDSVLASLDPL
GKQMIPGLRLPGVWSAFEAGCRAILGQQVSIIQASKLLNQLVLAYGEQRLIGAKQVTLFPLPETIAKAELNELKMPGVRK
SALNALGQFVSDNPEAEVEQWLSIKGIGPWTTAYAKMRGLSYPDVLLCGDLVVKKRVLSLYAAKADKLATSNGYDELTQE
LAKHVAPWGSYLTFQLWNLS
>Mature_500_residues
MDCGMLNHDCKIEREACRKARLARDPRFDGQFFIGVFTTKIYCRPICPAVSPKEENVRYFDSALQAANAGLRPCLRCRPD
SAPLSYAWKGTQTTLERAKVIIDAGELSGDDGLSVEALSERLGISSRYLRKLFSEKMGVSVKGYVQYRQLMFAKQLLHQT
QLSITQVAFAAGFNSIRRFNEVFKQALQLTPSALRKASTKRPPSDELQQQGQITLRLQLSYRPPLAWTQQHRFYEVRAVE
QMEWLEDPLSYGRSFTLNGVSGYFNAKLDAKANVFNVDIVLDNESGLSQLSFIISSIRRVLDLDANVLEIDSVLASLDPL
GKQMIPGLRLPGVWSAFEAGCRAILGQQVSIIQASKLLNQLVLAYGEQRLIGAKQVTLFPLPETIAKAELNELKMPGVRK
SALNALGQFVSDNPEAEVEQWLSIKGIGPWTTAYAKMRGLSYPDVLLCGDLVVKKRVLSLYAAKADKLATSNGYDELTQE
LAKHVAPWGSYLTFQLWNLS

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=292, Percent_Identity=30.1369863013699, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788542, Length=174, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 55811; Mature: 55811

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCGMLNHDCKIEREACRKARLARDPRFDGQFFIGVFTTKIYCRPICPAVSPKEENVRYF
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHHEECCCCCCCCCCCCHHHHH
DSALQAANAGLRPCLRCRPDSAPLSYAWKGTQTTLERAKVIIDAGELSGDDGLSVEALSE
HHHHHHHCCCCCCHHHCCCCCCCCCEEECCHHHHHHHHHEEEECCCCCCCCCCHHHHHHH
RLGISSRYLRKLFSEKMGVSVKGYVQYRQLMFAKQLLHQTQLSITQVAFAAGFNSIRRFN
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVFKQALQLTPSALRKASTKRPPSDELQQQGQITLRLQLSYRPPLAWTQQHRFYEVRAVE
HHHHHHHHCCHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
QMEWLEDPLSYGRSFTLNGVSGYFNAKLDAKANVFNVDIVLDNESGLSQLSFIISSIRRV
HHHHHHHHHHCCCEEEECCCCCEEECEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHH
LDLDANVLEIDSVLASLDPLGKQMIPGLRLPGVWSAFEAGCRAILGQQVSIIQASKLLNQ
HHCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LVLAYGEQRLIGAKQVTLFPLPETIAKAELNELKMPGVRKSALNALGQFVSDNPEAEVEQ
HHHHHCCHHCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHH
WLSIKGIGPWTTAYAKMRGLSYPDVLLCGDLVVKKRVLSLYAAKADKLATSNGYDELTQE
HHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAKHVAPWGSYLTFQLWNLS
HHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MDCGMLNHDCKIEREACRKARLARDPRFDGQFFIGVFTTKIYCRPICPAVSPKEENVRYF
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHHEECCCCCCCCCCCCHHHHH
DSALQAANAGLRPCLRCRPDSAPLSYAWKGTQTTLERAKVIIDAGELSGDDGLSVEALSE
HHHHHHHCCCCCCHHHCCCCCCCCCEEECCHHHHHHHHHEEEECCCCCCCCCCHHHHHHH
RLGISSRYLRKLFSEKMGVSVKGYVQYRQLMFAKQLLHQTQLSITQVAFAAGFNSIRRFN
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVFKQALQLTPSALRKASTKRPPSDELQQQGQITLRLQLSYRPPLAWTQQHRFYEVRAVE
HHHHHHHHCCHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
QMEWLEDPLSYGRSFTLNGVSGYFNAKLDAKANVFNVDIVLDNESGLSQLSFIISSIRRV
HHHHHHHHHHCCCEEEECCCCCEEECEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHH
LDLDANVLEIDSVLASLDPLGKQMIPGLRLPGVWSAFEAGCRAILGQQVSIIQASKLLNQ
HHCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LVLAYGEQRLIGAKQVTLFPLPETIAKAELNELKMPGVRKSALNALGQFVSDNPEAEVEQ
HHHHHCCHHCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHH
WLSIKGIGPWTTAYAKMRGLSYPDVLLCGDLVVKKRVLSLYAAKADKLATSNGYDELTQE
HHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAKHVAPWGSYLTFQLWNLS
HHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]