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The map label for this gene is ogt [H]

Identifier: 167623473

GI number: 167623473

Start: 1863827

End: 1864390

Strand: Direct

Name: ogt [H]

Synonym: Shal_1542

Alternate gene names: 167623473

Gene position: 1863827-1864390 (Clockwise)

Preceding gene: 167623472

Following gene: 167623474

Centisome position: 35.66

GC content: 45.92

Gene sequence:

>564_bases
ATGAATAAACCGTTACTGCCATTAGCTAAATTGAATTTTAAATCCGAGTTCGGCGCGCTCTCTGTGTGCGCTAATGCAGA
GGGCATCACTCACGTTAACTTTATTCCACAGCAAGGGGATGAATGGTTATCAAGAGAAGTTGATACTAACCAAGCATTAA
TGGGCGCTGGCGCATGTGCAGAGAATGGCGGTTGCGATAGTTATCATCTCGCCAAACAACACCTTTTACAGGCTCAGCTT
GAGCTGACGGAGTACTTTGATGGAGCGCGTCGCCAATTTGAGGTAAAACTCGCCCCTAAAGGCACTGAGTTTCAATGTCA
GGTTTGGCATGCATTAAGTGAGCTTGAATATGGCGAAAGCTGCAGCTACGCAGATATTGCTAGTCGTATTAATCGTCCTA
AAGCGGTGAGAGCGGTGGGCACCGCAAATGGCGCCAATCCCATTGCGATTATTGTGCCTTGCCATAGAGTGATAGGTAAA
AATGGTACACTTACTGGCTATGCTTATGGCATCGACTTGAAGCAGAAATTACTTAAACTCGAAGGCTTAAACTCGAAGGG
ATAG

Upstream 100 bases:

>100_bases
AGTTAGCAACATCAAATGGCTATGATGAGCTAACTCAAGAGCTTGCCAAACACGTTGCTCCATGGGGCAGTTATCTCACA
TTTCAGCTTTGGAACCTTTC

Downstream 100 bases:

>100_bases
TCTAATTAGGAATTGAGAGAAGTTGAGATAAGTTGAGGGTGACTCGAGTGAGGGCTGGGGTTTGAAATCATCTGGTCAAT
ACCCGCTTTAAAATGTCAGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNKPLLPLAKLNFKSEFGALSVCANAEGITHVNFIPQQGDEWLSREVDTNQALMGAGACAENGGCDSYHLAKQHLLQAQL
ELTEYFDGARRQFEVKLAPKGTEFQCQVWHALSELEYGESCSYADIASRINRPKAVRAVGTANGANPIAIIVPCHRVIGK
NGTLTGYAYGIDLKQKLLKLEGLNSKG

Sequences:

>Translated_187_residues
MNKPLLPLAKLNFKSEFGALSVCANAEGITHVNFIPQQGDEWLSREVDTNQALMGAGACAENGGCDSYHLAKQHLLQAQL
ELTEYFDGARRQFEVKLAPKGTEFQCQVWHALSELEYGESCSYADIASRINRPKAVRAVGTANGANPIAIIVPCHRVIGK
NGTLTGYAYGIDLKQKLLKLEGLNSKG
>Mature_187_residues
MNKPLLPLAKLNFKSEFGALSVCANAEGITHVNFIPQQGDEWLSREVDTNQALMGAGACAENGGCDSYHLAKQHLLQAQL
ELTEYFDGARRQFEVKLAPKGTEFQCQVWHALSELEYGESCSYADIASRINRPKAVRAVGTANGANPIAIIVPCHRVIGK
NGTLTGYAYGIDLKQKLLKLEGLNSKG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=75, Percent_Identity=41.3333333333333, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=49.5145631067961, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788542, Length=87, Percent_Identity=50.5747126436782, Blast_Score=97, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=51.8518518518518, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=51.8518518518518, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=49.4117647058824, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137554, Length=84, Percent_Identity=47.6190476190476, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20334; Mature: 20334

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00374 MGMT ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPLLPLAKLNFKSEFGALSVCANAEGITHVNFIPQQGDEWLSREVDTNQALMGAGACA
CCCCCCCHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCHHHHCCCCCC
ENGGCDSYHLAKQHLLQAQLELTEYFDGARRQFEVKLAPKGTEFQCQVWHALSELEYGES
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHCCCCCC
CSYADIASRINRPKAVRAVGTANGANPIAIIVPCHRVIGKNGTLTGYAYGIDLKQKLLKL
CCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECCCHHHHHHHH
EGLNSKG
CCCCCCC
>Mature Secondary Structure
MNKPLLPLAKLNFKSEFGALSVCANAEGITHVNFIPQQGDEWLSREVDTNQALMGAGACA
CCCCCCCHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCHHHHCCCCCC
ENGGCDSYHLAKQHLLQAQLELTEYFDGARRQFEVKLAPKGTEFQCQVWHALSELEYGES
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHCCCCCC
CSYADIASRINRPKAVRAVGTANGANPIAIIVPCHRVIGKNGTLTGYAYGIDLKQKLLKL
CCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECCCHHHHHHHH
EGLNSKG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]