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The map label for this gene is gltX

Identifier: 167623470

GI number: 167623470

Start: 1856456

End: 1857865

Strand: Direct

Name: gltX

Synonym: Shal_1539

Alternate gene names: 167623470

Gene position: 1856456-1857865 (Clockwise)

Preceding gene: 167623468

Following gene: 167623472

Centisome position: 35.52

GC content: 41.99

Gene sequence:

>1410_bases
ATGACAATTAAGACGCGTTTTGCTCCAAGCCCTACTGGCTTTTTGCACGTTGGTGGTGCTCGTACAGCACTTTATTCATG
GTTATATGCACGCGCAAACCAAGGTGAGTTTGTTTTACGTATTGAAGATACCGATATTGAACGTTCAACTCCTGAAGCAT
GTGCCGCTATTTTAGAAGGCATGGAGTGGCTTAACCTTAACTGGGATGAAGGTCCATATTATCAAACCAAGCGTTTTGAC
CGTTACAACGAAATCATTGCACAGATGCTAGAGCAGGGGACTGCTTATAAGTGTTATTGTAGTCGTGAGCGTATTGAAAC
GATGCGTGAAGAGCAAGCTGCAAACGGTGAACAGCAAAAATATGATGGCTGTTGTCGTGATAAGGCTCCACGCGATACAG
ATGAACCATTTGTAGTGCGTTTCAAAAACCCTACAGAAGGTAGTGTTGTTTTTGATGATCATGTACGTGGCCGTATCGAA
TTTGCAAACAGTACATTAGATGATCTAATTATCGCACGTACGGAAGGTACGCCTACTTATAACTTCTGTGTGGTTGTTGA
CGATTGGGATATGGGGATTACTTGTGTTGTACGTGGTGAAGACCATATCAACAATACGCCTCGTCAAATAAACATCCTTA
AAGCACTTGGCGCACCAGTACCTGAATATGCTCATGTTGCTATGATCCTAGGTGATGATGGAGCTAAATTATCTAAGCGT
CATGGTGCAGTCGGTGTGATGCAGTACCGCGATGACGGTTTCTTGCCAGAGGCACTGCTTAACTACCTTGTTCGTTTAGG
CTGGTCTCATGGAGATCAAGAAGTGTTCTCAATTGAAGAGATGAAGCAGTTCTTTAGCCTTGATGATATCAATAAAGCAG
CTTCTGCTTTTAATACAGATAAACTGATTTGGTTGAACCAGCACTATATTAAAGAAATGGATCCAGAATATGTAGCTTCT
CATCTTGAGTGGCACATGGCTGATCAAAACATTGATACAAGCAATGGTCCAAAACTTTCTGAAGTGGTTTCAGCTTTATC
AGAACGTGCTAAGACATTAAAAGAGCTTGCTGCTTCTAGTCGCTATTTCTTCGAAGACTTCGCTGAGTTTGAAGAAACTG
CCGCTAAAAAACACCTTAGAGGTGTAGCACTTGAGCCACTAACTCTTATTCAGTCTAAGTTAGCTGCATTAAATGAATGG
ACTTTAGAAGCTATTCACCAGGCAATTGAAGATACAGCGAGTGAATTAGACGTTGGAATGGGTAAAGTCGGTATGCCATT
GCGCGTTGCTGTTACCGGCGCAGGTATGTCACCTGCTGTAGATTTAACCTTATTCTTGGTTGGAAAGGCTCGTTGCGAAC
AAAGAATCTCCAAAGCGATTGAATTTGTAGCAAATAGAGTAAATTCATAA

Upstream 100 bases:

>100_bases
GTAGATCTATTTTCCCTAGCCCTTTTGGCTGTTAGAATATGCGCCACATTCATTAGTACAGTCTATTATTTAGACTTTCT
ACATAGGTCAGTATCCCATT

Downstream 100 bases:

>100_bases
AAAACGGGTTGACTCTTTTGGGTGCGCTGCATAGAATGCGCCACGCAGTTGAGACACAGGCAACGAATAGTTCGCGGTGC
ACTCAAAAGTGTTTTAGTTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTIKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDIERSTPEACAAILEGMEWLNLNWDEGPYYQTKRFD
RYNEIIAQMLEQGTAYKCYCSRERIETMREEQAANGEQQKYDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIE
FANSTLDDLIIARTEGTPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVAMILGDDGAKLSKR
HGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEVFSIEEMKQFFSLDDINKAASAFNTDKLIWLNQHYIKEMDPEYVAS
HLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASSRYFFEDFAEFEETAAKKHLRGVALEPLTLIQSKLAALNEW
TLEAIHQAIEDTASELDVGMGKVGMPLRVAVTGAGMSPAVDLTLFLVGKARCEQRISKAIEFVANRVNS

Sequences:

>Translated_469_residues
MTIKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDIERSTPEACAAILEGMEWLNLNWDEGPYYQTKRFD
RYNEIIAQMLEQGTAYKCYCSRERIETMREEQAANGEQQKYDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIE
FANSTLDDLIIARTEGTPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVAMILGDDGAKLSKR
HGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEVFSIEEMKQFFSLDDINKAASAFNTDKLIWLNQHYIKEMDPEYVAS
HLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASSRYFFEDFAEFEETAAKKHLRGVALEPLTLIQSKLAALNEW
TLEAIHQAIEDTASELDVGMGKVGMPLRVAVTGAGMSPAVDLTLFLVGKARCEQRISKAIEFVANRVNS
>Mature_468_residues
TIKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDIERSTPEACAAILEGMEWLNLNWDEGPYYQTKRFDR
YNEIIAQMLEQGTAYKCYCSRERIETMREEQAANGEQQKYDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIEF
ANSTLDDLIIARTEGTPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVAMILGDDGAKLSKRH
GAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEVFSIEEMKQFFSLDDINKAASAFNTDKLIWLNQHYIKEMDPEYVASH
LEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASSRYFFEDFAEFEETAAKKHLRGVALEPLTLIQSKLAALNEWT
LEAIHQAIEDTASELDVGMGKVGMPLRVAVTGAGMSPAVDLTLFLVGKARCEQRISKAIEFVANRVNS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=29.6, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=71.4285714285714, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=240, Percent_Identity=40.8333333333333, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786895, Length=220, Percent_Identity=26.3636363636364, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=29.9376299376299, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17511007, Length=379, Percent_Identity=24.2744063324538, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=30.4081632653061, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321192, Length=231, Percent_Identity=29.8701298701299, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=120, Percent_Identity=30, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=28.8577154308617, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SHEHH (B0TNB9)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673764.1
- ProteinModelPortal:   B0TNB9
- SMR:   B0TNB9
- GeneID:   5903706
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1539
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   SHAL458817:SHAL_1539-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52805; Mature: 52674

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDIERSTPEACAAILEG
CCEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCC
MEWLNLNWDEGPYYQTKRFDRYNEIIAQMLEQGTAYKCYCSRERIETMREEQAANGEQQK
HHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCHHH
YDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIEFANSTLDDLIIARTEGTPTY
HCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVAMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEVFSIEEMKQFFSLDDINKAASAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCC
KLIWLNQHYIKEMDPEYVASHLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASS
EEEEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RYFFEDFAEFEETAAKKHLRGVALEPLTLIQSKLAALNEWTLEAIHQAIEDTASELDVGM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVGMPLRVAVTGAGMSPAVDLTLFLVGKARCEQRISKAIEFVANRVNS
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDIERSTPEACAAILEG
CEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCC
MEWLNLNWDEGPYYQTKRFDRYNEIIAQMLEQGTAYKCYCSRERIETMREEQAANGEQQK
HHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCHHH
YDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIEFANSTLDDLIIARTEGTPTY
HCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVAMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEVFSIEEMKQFFSLDDINKAASAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCC
KLIWLNQHYIKEMDPEYVASHLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASS
EEEEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RYFFEDFAEFEETAAKKHLRGVALEPLTLIQSKLAALNEWTLEAIHQAIEDTASELDVGM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVGMPLRVAVTGAGMSPAVDLTLFLVGKARCEQRISKAIEFVANRVNS
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA