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The map label for this gene is aceF [H]

Identifier: 167622417

GI number: 167622417

Start: 566248

End: 567915

Strand: Direct

Name: aceF [H]

Synonym: Shal_0477

Alternate gene names: 167622417

Gene position: 566248-567915 (Clockwise)

Preceding gene: 167622416

Following gene: 167622418

Centisome position: 10.83

GC content: 49.22

Gene sequence:

>1668_bases
ATGGCTGAATTAAAAGAAGTTTTGATTCCTGATATCGGTGGCGATGAAGTTCAGGTTATTGAGATCTGTGCTGCGGTCGG
CGACACGCTTGCCGCTGAAGAGTCAATTATCACGGTTGAAAGTGATAAGGCGACGATGGATATCCCTGCACCTTTTGCTG
GCGTATTGAGCGAACTTAAAGTTGCGGTAGGTGATACGGTTTCAGAAGGTACACTTATCGCCATGATGTCTGCAGCGGGG
GCACAAGCTCCTACTGAGGTAGAGTCTACACCAGCTGCCCCTGACGCCGTTCAGGCTCCTGTTGCCGCTCAGGCTCCAGC
ACCAGAAGAAGCGCCAGCGGCTTCGGTTGCAAGCACAACTCAGGTGATTGAAGTTAGCGTGCCAGATATTGGTGATGCAA
GTGATGTCGATGTGATTGAAGTCTTAGTTGCCGTGGGCGACAAGATTGATGCCGATACTGGCTTAATTACTCTTGAGACA
GATAAAGCGACCATGGAGGTGCCAGCACCTTCGGCGGGTATCGTTAAAGAACTTAAAGTTGCCGTTGGCGACAAGGTATC
GATGGGTTCACTGGTATTGATGCTTGAAGTTAGCGATGCTGCTCCTGCAGCACCGGTTGTGGCAGCTCCTGCAACAAGCA
CTCCAGCTCCTGTTGCGCCAGCACCCGTTGCTCAAGCGGCCTCGGCAAACAAGCCGCCTGTGCCGCATCATCCAAGTGCG
GGCAGTCAGCCTAAAACCGGTGCCGTACATGCTTCACCTGCAGTACGTCGTTTAGCACGTGAGTTTGGTGCAGATCTTAC
TTTGGTAAAAGGTACGGGTCGTAAAGGACGTATTCTTAAGGAAGACGTACAGGCATTTATCAAGTATGAGCTCAGTCGTC
CTAAAGCCTCTGCTGCAACAGCCGTTGCAGGTGGCGCAGGTGGATTGAGTGTGATTGCTGCACCAAAAGTCGATTTTGCT
AAGTTTGGTGAAGTTGAAGAAGTGCCACTCAGTCGTATTCAGAAGATCTCTGGTCCAAACTTACATCGCAACTGGGTAAC
CATCCCACATGTGACGCAGTTTGATGAAGCTGATATCACTGAGCTTGAAGCATTCCGCAAGGATCAAAATGCCGTCGCTG
CAAAGAAGAAAGCGGATTATAAGATCACGCCTTTAGTCTTTATGATGAAAGCGGTGGCTAAAACCTTGGCTGAATTCCCA
GTGTTTAATTCAAGCTTGAGTGCCGATGGTGAATCACTCATCCAGAAGAAATACTTCCATATTGGTGTTGCGGTTGATAC
GCCAAATGGATTGATGGTGCCAGTGGTACGTGATGTCGATAAGAAAGGCATCGTAGAGCTATCACGTGAGTTAACGGAGA
TCTCTGTTAAGGCGCGTGACGGCAAGCTGAAAGCGGCTGATATGCAGGGTAGCTGTTTTACTATTTCAAGTCTTGGTGGT
ATTGGCGGCACGGCGTTTACGCCAATCGTTAACTACCCTGACGTTGCCATCTTGGGGGTGTCTAAGTCTGAGATTAAGCC
TAAGTGGAATGGTAAAGAGTTCGAGCCTAAGTTGATGTTGCCGTTGTCGTTGTCATACGATCACCGTGTGATTGATGGCG
CGATGGCTGCACGATTCAGTGTCACGCTTTCCAGCATTCTAAGTGATATTCGCACACTTATTTTGTAA

Upstream 100 bases:

>100_bases
TCGTAACGAGCTACCTGTTGATGTGCTAACTAAAGCTATCAAAGAGTATGGTATCGACGTTGACAAGATCAACCCACAGT
ACGCGTAAAGGGGAAACAAA

Downstream 100 bases:

>100_bases
AGTTAAAGGCTACTCAGATTGAGTAGCCTTTTTTCTAGATTGCTTAATTTTCAACTATTAATAATCATCACTAAGATTAA
GCGCTACTAGGATTGTGATC

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MAELKEVLIPDIGGDEVQVIEICAAVGDTLAAEESIITVESDKATMDIPAPFAGVLSELKVAVGDTVSEGTLIAMMSAAG
AQAPTEVESTPAAPDAVQAPVAAQAPAPEEAPAASVASTTQVIEVSVPDIGDASDVDVIEVLVAVGDKIDADTGLITLET
DKATMEVPAPSAGIVKELKVAVGDKVSMGSLVLMLEVSDAAPAAPVVAAPATSTPAPVAPAPVAQAASANKPPVPHHPSA
GSQPKTGAVHASPAVRRLAREFGADLTLVKGTGRKGRILKEDVQAFIKYELSRPKASAATAVAGGAGGLSVIAAPKVDFA
KFGEVEEVPLSRIQKISGPNLHRNWVTIPHVTQFDEADITELEAFRKDQNAVAAKKKADYKITPLVFMMKAVAKTLAEFP
VFNSSLSADGESLIQKKYFHIGVAVDTPNGLMVPVVRDVDKKGIVELSRELTEISVKARDGKLKAADMQGSCFTISSLGG
IGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSSILSDIRTLIL

Sequences:

>Translated_555_residues
MAELKEVLIPDIGGDEVQVIEICAAVGDTLAAEESIITVESDKATMDIPAPFAGVLSELKVAVGDTVSEGTLIAMMSAAG
AQAPTEVESTPAAPDAVQAPVAAQAPAPEEAPAASVASTTQVIEVSVPDIGDASDVDVIEVLVAVGDKIDADTGLITLET
DKATMEVPAPSAGIVKELKVAVGDKVSMGSLVLMLEVSDAAPAAPVVAAPATSTPAPVAPAPVAQAASANKPPVPHHPSA
GSQPKTGAVHASPAVRRLAREFGADLTLVKGTGRKGRILKEDVQAFIKYELSRPKASAATAVAGGAGGLSVIAAPKVDFA
KFGEVEEVPLSRIQKISGPNLHRNWVTIPHVTQFDEADITELEAFRKDQNAVAAKKKADYKITPLVFMMKAVAKTLAEFP
VFNSSLSADGESLIQKKYFHIGVAVDTPNGLMVPVVRDVDKKGIVELSRELTEISVKARDGKLKAADMQGSCFTISSLGG
IGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSSILSDIRTLIL
>Mature_554_residues
AELKEVLIPDIGGDEVQVIEICAAVGDTLAAEESIITVESDKATMDIPAPFAGVLSELKVAVGDTVSEGTLIAMMSAAGA
QAPTEVESTPAAPDAVQAPVAAQAPAPEEAPAASVASTTQVIEVSVPDIGDASDVDVIEVLVAVGDKIDADTGLITLETD
KATMEVPAPSAGIVKELKVAVGDKVSMGSLVLMLEVSDAAPAAPVVAAPATSTPAPVAPAPVAQAASANKPPVPHHPSAG
SQPKTGAVHASPAVRRLAREFGADLTLVKGTGRKGRILKEDVQAFIKYELSRPKASAATAVAGGAGGLSVIAAPKVDFAK
FGEVEEVPLSRIQKISGPNLHRNWVTIPHVTQFDEADITELEAFRKDQNAVAAKKKADYKITPLVFMMKAVAKTLAEFPV
FNSSLSADGESLIQKKYFHIGVAVDTPNGLMVPVVRDVDKKGIVELSRELTEISVKARDGKLKAADMQGSCFTISSLGGI
GGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSSILSDIRTLIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=452, Percent_Identity=28.3185840707965, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.8045977011494, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI203098816, Length=487, Percent_Identity=26.694045174538, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.8017621145374, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI203098753, Length=437, Percent_Identity=27.4599542334096, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI260898739, Length=150, Percent_Identity=34.6666666666667, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786305, Length=550, Percent_Identity=57.4545454545455, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=31.1059907834101, Blast_Score=176, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=30.0469483568075, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=31.0502283105023, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=458, Percent_Identity=31.0043668122271, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=32.2085889570552, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=28.2110091743119, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324258, Length=434, Percent_Identity=27.1889400921659, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI18859875, Length=443, Percent_Identity=29.7968397291196, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=35.0467289719626, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=29.7872340425532, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=29.7872340425532, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57631; Mature: 57499

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKEVLIPDIGGDEVQVIEICAAVGDTLAAEESIITVESDKATMDIPAPFAGVLSELK
CCCCHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDTVSEGTLIAMMSAAGAQAPTEVESTPAAPDAVQAPVAAQAPAPEEAPAASVASTT
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCE
QVIEVSVPDIGDASDVDVIEVLVAVGDKIDADTGLITLETDKATMEVPAPSAGIVKELKV
EEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHH
AVGDKVSMGSLVLMLEVSDAAPAAPVVAAPATSTPAPVAPAPVAQAASANKPPVPHHPSA
HHCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
GSQPKTGAVHASPAVRRLAREFGADLTLVKGTGRKGRILKEDVQAFIKYELSRPKASAAT
CCCCCCCCEECCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
AVAGGAGGLSVIAAPKVDFAKFGEVEEVPLSRIQKISGPNLHRNWVTIPHVTQFDEADIT
EECCCCCCEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHH
ELEAFRKDQNAVAAKKKADYKITPLVFMMKAVAKTLAEFPVFNSSLSADGESLIQKKYFH
HHHHHHCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
IGVAVDTPNGLMVPVVRDVDKKGIVELSRELTEISVKARDGKLKAADMQGSCFTISSLGG
EEEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHEEEEECCCCEEEECCCCCEEEEECCCC
IGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFS
CCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
VTLSSILSDIRTLIL
HHHHHHHHHHHHHHC
>Mature Secondary Structure 
AELKEVLIPDIGGDEVQVIEICAAVGDTLAAEESIITVESDKATMDIPAPFAGVLSELK
CCCHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDTVSEGTLIAMMSAAGAQAPTEVESTPAAPDAVQAPVAAQAPAPEEAPAASVASTT
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCE
QVIEVSVPDIGDASDVDVIEVLVAVGDKIDADTGLITLETDKATMEVPAPSAGIVKELKV
EEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHH
AVGDKVSMGSLVLMLEVSDAAPAAPVVAAPATSTPAPVAPAPVAQAASANKPPVPHHPSA
HHCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
GSQPKTGAVHASPAVRRLAREFGADLTLVKGTGRKGRILKEDVQAFIKYELSRPKASAAT
CCCCCCCCEECCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
AVAGGAGGLSVIAAPKVDFAKFGEVEEVPLSRIQKISGPNLHRNWVTIPHVTQFDEADIT
EECCCCCCEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHH
ELEAFRKDQNAVAAKKKADYKITPLVFMMKAVAKTLAEFPVFNSSLSADGESLIQKKYFH
HHHHHHCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
IGVAVDTPNGLMVPVVRDVDKKGIVELSRELTEISVKARDGKLKAADMQGSCFTISSLGG
EEEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHEEEEECCCCEEEECCCCCEEEEECCCC
IGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFS
CCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
VTLSSILSDIRTLIL
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]