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The map label for this gene is lpdA [H]

Identifier: 167622418

GI number: 167622418

Start: 568136

End: 569563

Strand: Direct

Name: lpdA [H]

Synonym: Shal_0478

Alternate gene names: 167622418

Gene position: 568136-569563 (Clockwise)

Preceding gene: 167622417

Following gene: 167622419

Centisome position: 10.87

GC content: 46.36

Gene sequence:

>1428_bases
ATGAGTAACGAAATCAAAACTCAGGTAGTTGTACTAGGCGCTGGCCCTGCTGGTTATTCTGCTGCATTCCGTGCTGCGGA
TTTAGGCCTAGATACTGTAATCGTTGAGCGTTTCAGTACATTGGGTGGTGTTTGTCTAAACGTAGGTTGTATCCCTTCTA
AAGCCTTGCTTCATGTTTCTAAAGTGATTGAAGAAGCTAAAGCTGTTGCCGATCACGGTGTCGTTTTTGGTGAACCGAAG
ATCGATCTTGATAAATTACGTGGCTTTAAAGAAAAAGTTATCGGTCAGCTCACTAGCGGCTTGGGCGGAATGTCTAAGAT
GCGTAAAGTTGACGTGGTTAATGGTCTAGGCAAATTCACAGGCCCTAACACGCTAGAAGTTCAAGGTGAAGATGGCGTTA
AGGTCGTTCACTTTGAGCAGGCGATTATTGCAGCGGGTTCTCGCCCAATTCAATTGCCATTTATCCCACACGAAGACCCA
CGTGTATGGGATTCGACTGACGCGCTAGAGCTTAAAGAAGTTCCTGGTAAGTTACTTGTTATGGGTGGCGGTATCATCGG
CCTAGAAATGGGCACCGTGTACTCATCACTCGGTAGTGAGATTGACGTGGTTGAGATGTTTGACCAAGTTATTCCAGCAG
CCGATAAAGACGTTGTTCGCGTATACACTAAGAAGATCAAGAAGAAGTTCAACTTGATGCTTGAAACGAAAGTGACTGCA
GTTGAAGCCAAAGAAGACGGCATCTATGTCACGATGGAAGGCAAGAAAGCACCTGCTGAACCTGTTCGTTATGATGCAGT
TTTGGTTGCCATTGGCCGTATTCCAAATGGCAAAGGCTTAGACGCTGAAAAAGCGGGTGTGAAGATTGATGAGCGTGGTT
TCATCAATGTTGATAAGCAGATGCGCACAAACGTGCCGCATATCTTCGCTATCGGTGACATCGTTGGTCAGCCAATGCTA
GCGCACAAAGGTGTGCATGAAGGCCATGTTGCTGCTGAAGTGATTTCAGGCCTTAAGCATTTCTTCGACCCTAAGGTTAT
CCCATCAATCGCCTATACCGATCCTGAAGTCGCATGGGTTGGTCTAACGGAGAAAGAAGCAAAAGATCAAGGTGTTGCTT
ACGAAACCGCGACTTTCCCATGGGCTGCAAGTGGCCGCGCTATCGCTTCTGATTGTGCTGACGGTATGACGAAGCTCATT
TTCGATAAAGAGACTCACCGTGTTATTGGTGGCGCTATTGTTGGTGTTAATGGTGGCGAACTATTAGGTGAAATCGGTCT
AGCAATTGAAATGGGTTGCGATGCGGAAGATCTCGCTCTAACTATCCATGCTCATCCAACATTGCATGAGTCTGTTGGCC
TAGCGGCTGAGATTTACGAAGGTTCTATCACCGATTTGCCAAACCCAAAGGCAAAGAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
ATGCGGCCACCTTACAAAGGATTTGGCCACAGTTTATCGTTGAGTCGGTACTTATCGTTAAGTCGATGCCGCCAACGACA
TAAAAAGAATAGAGGAAAAC

Downstream 100 bases:

>100_bases
GCTGTTAAGATAAATAGCTTCCAATCCAATAAAGCGCTCTTTTGAGCGCTTTATTATTTTCAGCTGCTCGGATGAATGAT
TTTTTAGGTAATCAAAGGGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLDTVIVERFSTLGGVCLNVGCIPSKALLHVSKVIEEAKAVADHGVVFGEPK
IDLDKLRGFKEKVIGQLTSGLGGMSKMRKVDVVNGLGKFTGPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRIPNGKGLDAEKAGVKIDERGFINVDKQMRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWVGLTEKEAKDQGVAYETATFPWAASGRAIASDCADGMTKLI
FDKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK

Sequences:

>Translated_475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLDTVIVERFSTLGGVCLNVGCIPSKALLHVSKVIEEAKAVADHGVVFGEPK
IDLDKLRGFKEKVIGQLTSGLGGMSKMRKVDVVNGLGKFTGPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRIPNGKGLDAEKAGVKIDERGFINVDKQMRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWVGLTEKEAKDQGVAYETATFPWAASGRAIASDCADGMTKLI
FDKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK
>Mature_474_residues
SNEIKTQVVVLGAGPAGYSAAFRAADLGLDTVIVERFSTLGGVCLNVGCIPSKALLHVSKVIEEAKAVADHGVVFGEPKI
DLDKLRGFKEKVIGQLTSGLGGMSKMRKVDVVNGLGKFTGPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDPR
VWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTAV
EAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRIPNGKGLDAEKAGVKIDERGFINVDKQMRTNVPHIFAIGDIVGQPMLA
HKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWVGLTEKEAKDQGVAYETATFPWAASGRAIASDCADGMTKLIF
DKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=41.6299559471366, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=27.8145695364238, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI148277065, Length=432, Percent_Identity=28.9351851851852, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519430, Length=432, Percent_Identity=28.9351851851852, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519428, Length=432, Percent_Identity=28.9351851851852, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519426, Length=432, Percent_Identity=28.9351851851852, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI148277071, Length=432, Percent_Identity=28.9351851851852, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI291045266, Length=448, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=27.9475982532751, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI291045268, Length=442, Percent_Identity=27.3755656108597, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=86.9198312236287, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=473, Percent_Identity=28.1183932346723, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=27.9120879120879, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI1789915, Length=447, Percent_Identity=28.4116331096197, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=39.8678414096916, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.9411764705882, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=443, Percent_Identity=28.2167042889391, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=443, Percent_Identity=28.2167042889391, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=448, Percent_Identity=25.8928571428571, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=149, Percent_Identity=30.8724832214765, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=40.3508771929825, Blast_Score=308, Evalue=9e-85,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=27.9317697228145, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=453, Percent_Identity=28.6975717439294, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.9190371991247, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24640549, Length=461, Percent_Identity=29.0672451193059, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24640553, Length=461, Percent_Identity=29.0672451193059, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640551, Length=461, Percent_Identity=29.7180043383948, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17737741, Length=485, Percent_Identity=26.8041237113402, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50609; Mature: 50478

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLDTVIVERFSTLGGVCLNVGCIPSKALLHVS
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVADHGVVFGEPKIDLDKLRGFKEKVIGQLTSGLGGMSKMRKVDVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
GPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKEVPGKLLV
CCCEEEEECCCCEEEEEECHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
EECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRIPNGKGLDAEKAGVKIDERGFINVDKQ
EEECCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCEECCCCCEEECHH
MRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKDQGVAYETATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGVNGGE
ECCHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNEIKTQVVVLGAGPAGYSAAFRAADLGLDTVIVERFSTLGGVCLNVGCIPSKALLHVS
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVADHGVVFGEPKIDLDKLRGFKEKVIGQLTSGLGGMSKMRKVDVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
GPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKEVPGKLLV
CCCEEEEECCCCEEEEEECHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
EECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRIPNGKGLDAEKAGVKIDERGFINVDKQ
EEECCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCEECCCCCEEECHH
MRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKDQGVAYETATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGVNGGE
ECCHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]