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The map label for this gene is ampE [H]

Identifier: 167622414

GI number: 167622414

Start: 561542

End: 562393

Strand: Direct

Name: ampE [H]

Synonym: Shal_0474

Alternate gene names: 167622414

Gene position: 561542-562393 (Clockwise)

Preceding gene: 167622413

Following gene: 167622415

Centisome position: 10.74

GC content: 46.24

Gene sequence:

>852_bases
ATGGCTTTATTTGCGTTATTAGTCGCAATTTTGGTTGAGCGATTACGACTTTTACCTAATGCTTGGCAGTTTGATGCCTT
GCTGTCTAAGTATCATAAGCCGCTGGTTAATGAGCAGCAGAAGCAATCGTTATCTATGGTGACATTGGCTATTTTGCTCC
CGGCTGCAATAGTCTATCTCTTGTCGTGGCTGGTATCAGGCCTATTGTGGGGATCTATTAGCCTAGCCCTATGGGTCGCG
ATAGCTGTGTTGTGCTTTAGCCATAACGCTCAAAGGCAGGCGTTTAAAAGTTATATACAAGCTGCTTGCCGGGCCGATCC
ACAAGCCTGTTTTCATTACGCAAACGAGTTGGACTCGAGTCAATGTCTAGATTCGGTAAATGAAAAAGAGTTGGGGGAGA
AGGTCGGTCAAAGTGTGGCGTGGATTAATTACCGTTACTATGGTGCTATCGCGCTTTACCTCATTATATTGGGGCCTGTT
GGTGCCGTGCTTTATTGTACTGTGCGTTTCTATAGTGAGCAGAGCCGTAAGAGTGAGGTGTCGCTTCCAGTCGTGGATAG
CTTGCTCTTTGTTTTAGATTGGCTCCCAAGCAGACTGTTTTCATTTGGTTATGTGCTGAGTGGCCACTTCAGATTGGCTA
TCTCTACTTGGCTCCCCCTTGCGCTGAACCCTAAATCTTCAGCTCGTGACATGATTACTCATGTCGCCGTTGCTGCCGAG
ACATTGCCTGAATCCTCGTCTGCACCCATTTGTTTGCAGTCGACTATCGCATTACTGCAACTGAGTAAGCGTAACTTCAT
CTTACTCATTACTGTACTGTCGCTAATGACCATATTTGGCGTAGTGAGTTAG

Upstream 100 bases:

>100_bases
GTAAATTGGGCGCTATATCTGACAATGCACTATTGTAGTAAGCACCATGCAGTAAGCTGTAAGCATTCAGCTATAGCGAG
TTAATATTAAGGTGAAAATT

Downstream 100 bases:

>100_bases
TAGGTAAATTGCAGGGGCACAAGAGAAAATAATTGCTAATCAAAAAATAGTTTTACACCGTTCGGATGTTCGATTATTGT
GCGATTAATTTGTTTTTTGT

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MALFALLVAILVERLRLLPNAWQFDALLSKYHKPLVNEQQKQSLSMVTLAILLPAAIVYLLSWLVSGLLWGSISLALWVA
IAVLCFSHNAQRQAFKSYIQAACRADPQACFHYANELDSSQCLDSVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPV
GAVLYCTVRFYSEQSRKSEVSLPVVDSLLFVLDWLPSRLFSFGYVLSGHFRLAISTWLPLALNPKSSARDMITHVAVAAE
TLPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS

Sequences:

>Translated_283_residues
MALFALLVAILVERLRLLPNAWQFDALLSKYHKPLVNEQQKQSLSMVTLAILLPAAIVYLLSWLVSGLLWGSISLALWVA
IAVLCFSHNAQRQAFKSYIQAACRADPQACFHYANELDSSQCLDSVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPV
GAVLYCTVRFYSEQSRKSEVSLPVVDSLLFVLDWLPSRLFSFGYVLSGHFRLAISTWLPLALNPKSSARDMITHVAVAAE
TLPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS
>Mature_282_residues
ALFALLVAILVERLRLLPNAWQFDALLSKYHKPLVNEQQKQSLSMVTLAILLPAAIVYLLSWLVSGLLWGSISLALWVAI
AVLCFSHNAQRQAFKSYIQAACRADPQACFHYANELDSSQCLDSVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVG
AVLYCTVRFYSEQSRKSEVSLPVVDSLLFVLDWLPSRLFSFGYVLSGHFRLAISTWLPLALNPKSSARDMITHVAVAAET
LPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=295, Percent_Identity=26.4406779661017, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 31386; Mature: 31255

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFALLVAILVERLRLLPNAWQFDALLSKYHKPLVNEQQKQSLSMVTLAILLPAAIVYL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSWLVSGLLWGSISLALWVAIAVLCFSHNAQRQAFKSYIQAACRADPQACFHYANELDSS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHH
QCLDSVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVGAVLYCTVRFYSEQSRKSEV
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLPVVDSLLFVLDWLPSRLFSFGYVLSGHFRLAISTWLPLALNPKSSARDMITHVAVAAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHH
TLPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS
HCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFALLVAILVERLRLLPNAWQFDALLSKYHKPLVNEQQKQSLSMVTLAILLPAAIVYL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSWLVSGLLWGSISLALWVAIAVLCFSHNAQRQAFKSYIQAACRADPQACFHYANELDSS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHH
QCLDSVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVGAVLYCTVRFYSEQSRKSEV
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLPVVDSLLFVLDWLPSRLFSFGYVLSGHFRLAISTWLPLALNPKSSARDMITHVAVAAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHH
TLPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS
HCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]