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The map label for this gene is ampD [H]

Identifier: 167622413

GI number: 167622413

Start: 560903

End: 561445

Strand: Direct

Name: ampD [H]

Synonym: Shal_0473

Alternate gene names: 167622413

Gene position: 560903-561445 (Clockwise)

Preceding gene: 167622403

Following gene: 167622414

Centisome position: 10.73

GC content: 43.65

Gene sequence:

>543_bases
TTGTCACTGACTTTACTTGATGGCTGGGTGACTCAGGCTCGATTTTGTCCCTCTCCGCATTTTAATATAAGACCAGATAA
TGAAGTGAGTTTGTTGGTTATCCATAATATCAGTTTGCCTGCGGGGTGTTTCGGTGAGCCTTATATCGACCAGTTATTTC
AAGGTTGTTTAGATATTGAGGCCGACAAGAGCTTTAAGGCATTACAAGGCTTAGCCGTTTCTGCACATTTCTTGATCCGC
CGTGATGGCGAGCTGGTGCAGTATGTTTGTTGTGATGATAGGGCTTGGCACGCAGGCGTATCTGAATTTGAACGTCGTAG
CAATTGCAATGATTTTTCGATTGGTATTGAACTTGAAGGCACGGATAGCCTTACTTATCCAGATCTGCAGTATCAAAGCT
TGATTGAGCTGACTAAGAGTTTGATGATCCACTATCCTGAAATTACGTCGACACGAATTACAGGTCACAGTGATATTGCA
CCGGGGCGAAAAACAGATCCCGGTAATAGTTTTGATTGGCAGCGCTATAAAGCTGCGTTGTAA

Upstream 100 bases:

>100_bases
TGTTTGTATTTTTAGGATGATAAATCTCAAAAGCGTGACTATATTCATAGGGTTAAAACTGCTAAAGTAAGTTCAATATT
TTTGCTTTAGGAACGCTCTT

Downstream 100 bases:

>100_bases
ATTGGGCGCTATATCTGACAATGCACTATTGTAGTAAGCACCATGCAGTAAGCTGTAAGCATTCAGCTATAGCGAGTTAA
TATTAAGGTGAAAATTATGG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSLTLLDGWVTQARFCPSPHFNIRPDNEVSLLVIHNISLPAGCFGEPYIDQLFQGCLDIEADKSFKALQGLAVSAHFLIR
RDGELVQYVCCDDRAWHAGVSEFERRSNCNDFSIGIELEGTDSLTYPDLQYQSLIELTKSLMIHYPEITSTRITGHSDIA
PGRKTDPGNSFDWQRYKAAL

Sequences:

>Translated_180_residues
MSLTLLDGWVTQARFCPSPHFNIRPDNEVSLLVIHNISLPAGCFGEPYIDQLFQGCLDIEADKSFKALQGLAVSAHFLIR
RDGELVQYVCCDDRAWHAGVSEFERRSNCNDFSIGIELEGTDSLTYPDLQYQSLIELTKSLMIHYPEITSTRITGHSDIA
PGRKTDPGNSFDWQRYKAAL
>Mature_179_residues
SLTLLDGWVTQARFCPSPHFNIRPDNEVSLLVIHNISLPAGCFGEPYIDQLFQGCLDIEADKSFKALQGLAVSAHFLIRR
DGELVQYVCCDDRAWHAGVSEFERRSNCNDFSIGIELEGTDSLTYPDLQYQSLIELTKSLMIHYPEITSTRITGHSDIAP
GRKTDPGNSFDWQRYKAAL

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=176, Percent_Identity=58.5227272727273, Blast_Score=211, Evalue=2e-56,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=36.1538461538462, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20271; Mature: 20140

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTLLDGWVTQARFCPSPHFNIRPDNEVSLLVIHNISLPAGCFGEPYIDQLFQGCLDIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
ADKSFKALQGLAVSAHFLIRRDGELVQYVCCDDRAWHAGVSEFERRSNCNDFSIGIELEG
CCCCHHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECC
TDSLTYPDLQYQSLIELTKSLMIHYPEITSTRITGHSDIAPGRKTDPGNSFDWQRYKAAL
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SLTLLDGWVTQARFCPSPHFNIRPDNEVSLLVIHNISLPAGCFGEPYIDQLFQGCLDIE
EEEEECCHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
ADKSFKALQGLAVSAHFLIRRDGELVQYVCCDDRAWHAGVSEFERRSNCNDFSIGIELEG
CCCCHHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECC
TDSLTYPDLQYQSLIELTKSLMIHYPEITSTRITGHSDIAPGRKTDPGNSFDWQRYKAAL
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]