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The map label for this gene is pyrE [H]

Identifier: 167622363

GI number: 167622363

Start: 498083

End: 498724

Strand: Direct

Name: pyrE [H]

Synonym: Shal_0423

Alternate gene names: 167622363

Gene position: 498083-498724 (Clockwise)

Preceding gene: 167622362

Following gene: 167622369

Centisome position: 9.53

GC content: 47.82

Gene sequence:

>642_bases
GTGAAAGCCTATCAACGTGAGTTTATTGAATTTGCCCTAGAACGTCAGGTACTTCGATTTGGCGAATTTACGTTAAAGTC
AGGCCGCACTAGCCCTTATTTCTTTAACGCGGGCCTGTTTAACACGGGTCGTGATTTGGCGCGTCTAGGTCGTTTCTATG
CTGCAGCCTTGGTTGACTCTGGTATTGAGTATGACTTGCTATTTGGCCCTGCCTACAAAGGCATTCCAATTGCTACTACG
ACTGCGGTCGCCCTGTGTGATCACCATGATATCGATATGCCTTACTGCTTTAACCGCAAAGAGAAGAAAGATCACGGCGA
AGGTGGCAGCTTAGTGGGTAGCGAGCTTCAAGGCCGCGTAATGCTAGTCGATGATGTGATCACTGCGGGCACGGCTATTC
GTGAGTCTATGGAGATCATTGCAGCACATAAGGCATCACTGGCTGGTGTGTTGATTGCACTTGATCGCCAAGAGAAGGGC
AAGGGCGAGCTTTCTGCGATTCAAGAAGTTGAACGTGACTTTGGTTGTGACATCGTTTCAATCATCAAGCTTGAAGACTT
GATTAACTACTTGTCTGAAAAGTCAGGTATGGAAGCGCAGCTTGCATCTGTTAGCGCATACCGCGATCAGTACGGGATTT
AA

Upstream 100 bases:

>100_bases
GGCCCTACGGGGTCCTTTTTGTAAGTAAAAAAAATCAACTAATACTAAATGCTACTAAATAATACATAGCGTATTACCCC
TACATTTTTAAGGAGAGATT

Downstream 100 bases:

>100_bases
GGTTTTTAGGTCCTAGGTCCTAGGTTCTAGGTTCTAGGTAAAGCACCTTAACCTTGTAGGTCGGCATTTATGCCGTCAGT
TATTTTGATTTTGTCATTAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKAYQREFIEFALERQVLRFGEFTLKSGRTSPYFFNAGLFNTGRDLARLGRFYAAALVDSGIEYDLLFGPAYKGIPIATT
TAVALCDHHDIDMPYCFNRKEKKDHGEGGSLVGSELQGRVMLVDDVITAGTAIRESMEIIAAHKASLAGVLIALDRQEKG
KGELSAIQEVERDFGCDIVSIIKLEDLINYLSEKSGMEAQLASVSAYRDQYGI

Sequences:

>Translated_213_residues
MKAYQREFIEFALERQVLRFGEFTLKSGRTSPYFFNAGLFNTGRDLARLGRFYAAALVDSGIEYDLLFGPAYKGIPIATT
TAVALCDHHDIDMPYCFNRKEKKDHGEGGSLVGSELQGRVMLVDDVITAGTAIRESMEIIAAHKASLAGVLIALDRQEKG
KGELSAIQEVERDFGCDIVSIIKLEDLINYLSEKSGMEAQLASVSAYRDQYGI
>Mature_213_residues
MKAYQREFIEFALERQVLRFGEFTLKSGRTSPYFFNAGLFNTGRDLARLGRFYAAALVDSGIEYDLLFGPAYKGIPIATT
TAVALCDHHDIDMPYCFNRKEKKDHGEGGSLVGSELQGRVMLVDDVITAGTAIRESMEIIAAHKASLAGVLIALDRQEKG
KGELSAIQEVERDFGCDIVSIIKLEDLINYLSEKSGMEAQLASVSAYRDQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=187, Percent_Identity=26.7379679144385, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=79.3427230046948, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17508631, Length=195, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=191, Percent_Identity=24.6073298429319, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=47.7272727272727, Blast_Score=201, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=43.4389140271493, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23531; Mature: 23531

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYQREFIEFALERQVLRFGEFTLKSGRTSPYFFNAGLFNTGRDLARLGRFYAAALVDS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIEYDLLFGPAYKGIPIATTTAVALCDHHDIDMPYCFNRKEKKDHGEGGSLVGSELQGRV
CCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMEIIAAHKASLAGVLIALDRQEKGKGELSAIQEVERDFGCDIVS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHH
IIKLEDLINYLSEKSGMEAQLASVSAYRDQYGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAYQREFIEFALERQVLRFGEFTLKSGRTSPYFFNAGLFNTGRDLARLGRFYAAALVDS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIEYDLLFGPAYKGIPIATTTAVALCDHHDIDMPYCFNRKEKKDHGEGGSLVGSELQGRV
CCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMEIIAAHKASLAGVLIALDRQEKGKGELSAIQEVERDFGCDIVS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHH
IIKLEDLINYLSEKSGMEAQLASVSAYRDQYGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]