Click here to switch to the map view.

The map label for this gene is mdtL [H]

Identifier: 167622253

GI number: 167622253

Start: 370609

End: 371811

Strand: Direct

Name: mdtL [H]

Synonym: Shal_0312

Alternate gene names: 167622253

Gene position: 370609-371811 (Clockwise)

Preceding gene: 167622250

Following gene: 167622254

Centisome position: 7.09

GC content: 49.79

Gene sequence:

>1203_bases
ATGCGTCGCAGTTTGCTACCTATTTTATTACCAATGGTGATCCTAAGCCCATTGGCTATCGATATTTACCTTCCCTCCAT
GCCTGTCATGGCGGCTGAGTTTTCGGTGTCTGCCAGTGAGATCCAATCGACACTGGTGCTTTTCCTGTTCGCCATGGGTA
CAGGCCAGTTATTAATTGGCCCTCTTGCTGATCGTTATGGTCGCCGCCCCGTTGCCATTGGCGGTATCATTTTTTATATG
ATTAGCAGTGTATTAGCTGCTATTGCGGTAGAGTTTCACTGGCTGCAAATCGCCCGAGTGATGCAGGGCCTAGCGGCGTG
TTCAACCTCGATAGTGGTATTTAGTGCCGTTAGAGACTGCTTCTCCCCGAAAGAGGGCGCACGCTACTACAGCTATCTAA
ACGGCATGATCTGTATTATTCCGGCCTTAGCACCGACGCTAGGCGGCATGTTAGCACTGCAGTTTGGTTGGCGATCTAAC
TTCATCTTTATGGCGCTGTATGGCCTAGTTATTTTGGCTTTGGTGAGCCTGCGTTTACCTGAAACTCGTCCACAAAATAC
CGTGAGTGATGGCCCGCTATATCGCTGGGCACGTTATAAGCCTGTGATCACCGAGCCGCACTTCCTGTTTTACGCCATCT
CTTGTATGGCGGGTATGGCAGCGATTCTTTGTTATGTCTCTTACGCGCCAGTCTGGCTTATCGGACACCTAGGTGTGTCG
GAGCTCACTTTCAGTGGTCTGTTTGGTTTAAATGCCGTGGTAAATATCATTGCCTGTTTTACCGCGCCCTTAGTGATTAA
GAAGTTAGGCAACCGACCAACCGTTATTTTGGCCTTGGTATTGATGCTGGTATCGGCGGTGACTCAGGTCGTTGTGCAGC
TAGTTGGGCCGCAAACAGGCTTGATGGCAGCCTATAGTTATATGCTACCTATGATGCTGCTATGTATCGGTTTTGCCCTA
TTACTTGGCCCTGCAACCAGCATGGCGTTATCAGCCTTTGGTGAAAGAGCGGGTACAGCAACGGCAATGCTTGGATTCAT
TCAGATGAGTGGCGCCTCATTGCTGACGGCACTTGTGCAGCAAACGAGTCTACCTGCCCCATATGCAGTAGCGCTTTTGA
TGGGCACAGGAACCATAGCGTTACTCGCTATGATGGCGATGCCAAAGTTTGATCACTGGCATCAAGAGCAGCACGCTTAT
TAA

Upstream 100 bases:

>100_bases
AGATATTCCTCAATGGAAATTCGTTATGGAAATAATATCATTTCTGTTCATACCATAGTTTGATTACTATAGCGCTGTTT
TCCTTATTCTGAGAAAGATC

Downstream 100 bases:

>100_bases
AGGTTTGCTTGCCCCATATTGATTCACCCTAAGCCTGGATAGATTAACCACCTATGCGGGCTTTTTTTACACTAAATTTA
TTGCGATTGTAATGTCGAGT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRRSLLPILLPMVILSPLAIDIYLPSMPVMAAEFSVSASEIQSTLVLFLFAMGTGQLLIGPLADRYGRRPVAIGGIIFYM
ISSVLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDCFSPKEGARYYSYLNGMICIIPALAPTLGGMLALQFGWRSN
FIFMALYGLVILALVSLRLPETRPQNTVSDGPLYRWARYKPVITEPHFLFYAISCMAGMAAILCYVSYAPVWLIGHLGVS
ELTFSGLFGLNAVVNIIACFTAPLVIKKLGNRPTVILALVLMLVSAVTQVVVQLVGPQTGLMAAYSYMLPMMLLCIGFAL
LLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALVQQTSLPAPYAVALLMGTGTIALLAMMAMPKFDHWHQEQHAY

Sequences:

>Translated_400_residues
MRRSLLPILLPMVILSPLAIDIYLPSMPVMAAEFSVSASEIQSTLVLFLFAMGTGQLLIGPLADRYGRRPVAIGGIIFYM
ISSVLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDCFSPKEGARYYSYLNGMICIIPALAPTLGGMLALQFGWRSN
FIFMALYGLVILALVSLRLPETRPQNTVSDGPLYRWARYKPVITEPHFLFYAISCMAGMAAILCYVSYAPVWLIGHLGVS
ELTFSGLFGLNAVVNIIACFTAPLVIKKLGNRPTVILALVLMLVSAVTQVVVQLVGPQTGLMAAYSYMLPMMLLCIGFAL
LLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALVQQTSLPAPYAVALLMGTGTIALLAMMAMPKFDHWHQEQHAY
>Mature_400_residues
MRRSLLPILLPMVILSPLAIDIYLPSMPVMAAEFSVSASEIQSTLVLFLFAMGTGQLLIGPLADRYGRRPVAIGGIIFYM
ISSVLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDCFSPKEGARYYSYLNGMICIIPALAPTLGGMLALQFGWRSN
FIFMALYGLVILALVSLRLPETRPQNTVSDGPLYRWARYKPVITEPHFLFYAISCMAGMAAILCYVSYAPVWLIGHLGVS
ELTFSGLFGLNAVVNIIACFTAPLVIKKLGNRPTVILALVLMLVSAVTQVVVQLVGPQTGLMAAYSYMLPMMLLCIGFAL
LLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALVQQTSLPAPYAVALLMGTGTIALLAMMAMPKFDHWHQEQHAY

Specific function: Confers resistance to chloramphenicol [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=394, Percent_Identity=30.9644670050761, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082312, Length=345, Percent_Identity=28.4057971014493, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788509, Length=373, Percent_Identity=26.2734584450402, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994889, Length=190, Percent_Identity=30.5263157894737, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790794, Length=248, Percent_Identity=26.2096774193548, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787065, Length=334, Percent_Identity=22.7544910179641, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=168, Percent_Identity=25.5952380952381, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322071, Length=170, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43166; Mature: 43166

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSLLPILLPMVILSPLAIDIYLPSMPVMAAEFSVSASEIQSTLVLFLFAMGTGQLLIG
CCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
PLADRYGRRPVAIGGIIFYMISSVLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSPKEGARYYSYLNGMICIIPALAPTLGGMLALQFGWRSNFIFMALYGLVILALVSLRLP
CCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHCCC
ETRPQNTVSDGPLYRWARYKPVITEPHFLFYAISCMAGMAAILCYVSYAPVWLIGHLGVS
CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELTFSGLFGLNAVVNIIACFTAPLVIKKLGNRPTVILALVLMLVSAVTQVVVQLVGPQTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LMAAYSYMLPMMLLCIGFALLLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALVQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHH
QTSLPAPYAVALLMGTGTIALLAMMAMPKFDHWHQEQHAY
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
>Mature Secondary Structure
MRRSLLPILLPMVILSPLAIDIYLPSMPVMAAEFSVSASEIQSTLVLFLFAMGTGQLLIG
CCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
PLADRYGRRPVAIGGIIFYMISSVLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSPKEGARYYSYLNGMICIIPALAPTLGGMLALQFGWRSNFIFMALYGLVILALVSLRLP
CCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHCCC
ETRPQNTVSDGPLYRWARYKPVITEPHFLFYAISCMAGMAAILCYVSYAPVWLIGHLGVS
CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELTFSGLFGLNAVVNIIACFTAPLVIKKLGNRPTVILALVLMLVSAVTQVVVQLVGPQTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LMAAYSYMLPMMLLCIGFALLLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALVQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHH
QTSLPAPYAVALLMGTGTIALLAMMAMPKFDHWHQEQHAY
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA