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The map label for this gene is nrfD [H]

Identifier: 167622244

GI number: 167622244

Start: 362537

End: 363478

Strand: Direct

Name: nrfD [H]

Synonym: Shal_0303

Alternate gene names: 167622244

Gene position: 362537-363478 (Clockwise)

Preceding gene: 167622243

Following gene: 167622245

Centisome position: 6.94

GC content: 45.44

Gene sequence:

>942_bases
ATGAGTCCATTTCATTTTGACAGCCTAATTTGGCATTGGCCGATTGCCATCTACCTATTTCTAGCGGGTGTATCCGCTGG
AGCAATCTGTTTCGCAGTATTGCTAAAACACTTTAAATTGGGACGTGATGCCTATAAATCGGGCTTTGTTCAGGCCGCAT
GTCTTATAGCACCGTTGGCTGTATTTGCAGGCCTTGGCATTTTAGTTATCGATTTAACTAAGCCGTTTGATTTTTGGAAA
ATCCTACTCTTCTACAACCCAACTTCTGTGATGTCCGTTGGTGTCGCTGTACTGATGGTCTATCAGGTAGCCCTGTTCGC
TTGGTTAGCTTGTGTCTTTAAAGAGCCTGCGCTTTCCTGGTGTAGCGGTCGCTGGCCAGTTGTTGAGAAGTTATTTAATG
CAATCGCTAAGCATGAAGCAACCATTACTGGCTTCTTGGTGATGCTTGCCTTGTCGTTAGGTGCGTATACCGGCTTCCTA
TTATCGGCATTAACGACCTATCCAATGCTAAATAATCCGGTATTGCCTTTGCTGTTTTTAGTCTCTGGTCTTTCATCGGG
AGCGGCGGGCACTCTACTTGGCGGCGTATTGCTCAAAGGTAATCCTGATGGTAAGGAAGTGCACTTTATCCACAATATTG
AGATCCCGATGATTTTGGTGGAAATTGTGCTGATCTTCACCTTCTTTGCCGGCTTGATTTTAACCGGAGGGCAGAGCCAA
GTGGCAGCATTTACTGCAATTGGAGAGGGCTTCTGGGGCTGGATTTTCTGGGCGGGTATCGTTGTTGTTGGTTTGAGTAT
GCCACTGGCCTTTAACCTATTTATGAAAGCATCTTCTAAGCGCAAGTTCTCTTATGTGGCTATTACAGCTAGCTGCAGTT
TAATCGGCGTGCTGTTACTGCGTAATTTCATTCTTTATACCGGCCAAATGACGGTTGCTTAA

Upstream 100 bases:

>100_bases
TACAAGTGCTTAAATCAACCCCTAGCTACCGCAGCAAAACCGATCTCGGGACTAGACCTAAGGTGTTCCGTATCGCGTCA
CAAAGCGGGGAGATAAAGTT

Downstream 100 bases:

>100_bases
TTATTTACTGAGGGTTTGGATATGCTTAAAGTTTTAGGGTTAACAGTTTTATTGAGCTTTTCTGGTGCTGCAATGGCGAC
TTCAGTTGCTGATTCGCATA

Product: polysulfide reductase NrfD

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSPFHFDSLIWHWPIAIYLFLAGVSAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLAVFAGLGILVIDLTKPFDFWK
ILLFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSWCSGRWPVVEKLFNAIAKHEATITGFLVMLALSLGAYTGFL
LSALTTYPMLNNPVLPLLFLVSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFAGLILTGGQSQ
VAAFTAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLLRNFILYTGQMTVA

Sequences:

>Translated_313_residues
MSPFHFDSLIWHWPIAIYLFLAGVSAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLAVFAGLGILVIDLTKPFDFWK
ILLFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSWCSGRWPVVEKLFNAIAKHEATITGFLVMLALSLGAYTGFL
LSALTTYPMLNNPVLPLLFLVSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFAGLILTGGQSQ
VAAFTAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLLRNFILYTGQMTVA
>Mature_312_residues
SPFHFDSLIWHWPIAIYLFLAGVSAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLAVFAGLGILVIDLTKPFDFWKI
LLFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSWCSGRWPVVEKLFNAIAKHEATITGFLVMLALSLGAYTGFLL
SALTTYPMLNNPVLPLLFLVSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFAGLILTGGQSQV
AAFTAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLLRNFILYTGQMTVA

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=319, Percent_Identity=44.8275862068966, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 33947; Mature: 33816

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFHFDSLIWHWPIAIYLFLAGVSAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLA
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFAGLGILVIDLTKPFDFWKILLFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSW
HHHCCCEEEEEECCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
CSGRWPVVEKLFNAIAKHEATITGFLVMLALSLGAYTGFLLSALTTYPMLNNPVLPLLFL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFAGLILTGGQSQ
HHCCCCCCHHHHHHCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
VAAFTAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHH
RNFILYTGQMTVA
HHHHHHCCCEECC
>Mature Secondary Structure 
SPFHFDSLIWHWPIAIYLFLAGVSAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLA
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFAGLGILVIDLTKPFDFWKILLFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSW
HHHCCCEEEEEECCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
CSGRWPVVEKLFNAIAKHEATITGFLVMLALSLGAYTGFLLSALTTYPMLNNPVLPLLFL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFAGLILTGGQSQ
HHCCCCCCHHHHHHCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
VAAFTAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHH
RNFILYTGQMTVA
HHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]