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The map label for this gene is fadH [C]

Identifier: 167622129

GI number: 167622129

Start: 221091

End: 222191

Strand: Direct

Name: fadH [C]

Synonym: Shal_0188

Alternate gene names: 167622129

Gene position: 221091-222191 (Clockwise)

Preceding gene: 167622122

Following gene: 167622130

Centisome position: 4.23

GC content: 48.59

Gene sequence:

>1101_bases
TTGAGCACTTTGTTTTCACCCGCCCGGATTGGCAAGATGACACTGAAAAACCGCTTTGTGAGAAGCGCGACTTGGGAAAA
TATGGCGACGGAAGATGGCCATATGACCGACAAGTTATATTCGGTGTATGAAGAGCTGGCTAAAGGCGAGGTGGGATTGA
TTGTCACGGGCTATGCCAATATTGTCGCCGAAGAGAAGCCCAATGCTGGCATGATGGGTATGTATAACGACTCGTTTATC
GCTGAATACCAGAAGCTGACCGAGTTAGTGCATAAGTATGATTCGAAAATCGTTATGCAGCTGGCGTATGGCGGCACTAA
GACCACCTACAATGTGGGTGAGCGAGTGATCTTCGCCCCGAGTGCAGTGGCAGAGCGCGGCACCCAAACACTGGGTAAGC
CAATGACCAAAACTGAGATAGACTATATCGCCTCAGCATTTGCACTGGCGAGCCGCAGAGCCCAAGAGTCCGGCTTTGAT
GGCGTTGAGATCCATGCGGCGCACACTTACCTGATTAACCAGTTCTTGAGTCCCTACTATAATCAGCGTGAAGATGAATA
CGGCGGCAGCCTTGAGAACCGTATGCGTTTCTTGCTGGATATTTACACCGAGATCAGAAAGCGAGTTGGCGAGAGTTATC
CTATTTTAGTCAAGTTAACCGCATCGGAGTTTTTCGACGGTGGCTTAACTTTTGATGAAACTCGTCTTATCTGTAAGAAG
CTAGAGTCGGTAGGTGTTGATGCCATTATAATTTCTGGCAACATTCATGGTAACGCCTGTACGGTAGTCGGTGAGTCGTT
TGACGGTCACGCTATTCAACAGGAAGGCTACTTCCGTGAATATGGCGCCGTTATAAGCCAAGAGGTAAATCTACCAATTA
TTACCGTTGGCGGACTTGCCGATTTCTGTGCCATCGAAGAGATCGCGGCGACCACCAATATTCAGTTCTTTGCGCTTTCA
AGACCTTTGCTTGCCGAGCCACAGCTCATAAAGCGTTGGTCAGATGGCCGTAGAGCGGAAGTAGATTGTGAGCGCTGCTC
AAAATGCCGCACTAAACGTGGTAATTTCTGTGTGGTGTATAAAAAGCGTAACGCCGCTTAA

Upstream 100 bases:

>100_bases
CATGGCTGTTGTGTTTTTGATCTTTGGCAAGGCTGGTGGATAGCCGACATACCAATCTGCAACAGCGATTGGTATAAATT
GATTTTTAAGAGGATTGCAC

Downstream 100 bases:

>100_bases
GCTTATGATTGCATGGTTGGCACGATTACTATCGTGCCAACCTCTTTATCTTTTTCCTAATTTTTACCCTAATGCTCCCC
ATCGTAGCCGCTTCGTTTTC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSTLFSPARIGKMTLKNRFVRSATWENMATEDGHMTDKLYSVYEELAKGEVGLIVTGYANIVAEEKPNAGMMGMYNDSFI
AEYQKLTELVHKYDSKIVMQLAYGGTKTTYNVGERVIFAPSAVAERGTQTLGKPMTKTEIDYIASAFALASRRAQESGFD
GVEIHAAHTYLINQFLSPYYNQREDEYGGSLENRMRFLLDIYTEIRKRVGESYPILVKLTASEFFDGGLTFDETRLICKK
LESVGVDAIIISGNIHGNACTVVGESFDGHAIQQEGYFREYGAVISQEVNLPIITVGGLADFCAIEEIAATTNIQFFALS
RPLLAEPQLIKRWSDGRRAEVDCERCSKCRTKRGNFCVVYKKRNAA

Sequences:

>Translated_366_residues
MSTLFSPARIGKMTLKNRFVRSATWENMATEDGHMTDKLYSVYEELAKGEVGLIVTGYANIVAEEKPNAGMMGMYNDSFI
AEYQKLTELVHKYDSKIVMQLAYGGTKTTYNVGERVIFAPSAVAERGTQTLGKPMTKTEIDYIASAFALASRRAQESGFD
GVEIHAAHTYLINQFLSPYYNQREDEYGGSLENRMRFLLDIYTEIRKRVGESYPILVKLTASEFFDGGLTFDETRLICKK
LESVGVDAIIISGNIHGNACTVVGESFDGHAIQQEGYFREYGAVISQEVNLPIITVGGLADFCAIEEIAATTNIQFFALS
RPLLAEPQLIKRWSDGRRAEVDCERCSKCRTKRGNFCVVYKKRNAA
>Mature_365_residues
STLFSPARIGKMTLKNRFVRSATWENMATEDGHMTDKLYSVYEELAKGEVGLIVTGYANIVAEEKPNAGMMGMYNDSFIA
EYQKLTELVHKYDSKIVMQLAYGGTKTTYNVGERVIFAPSAVAERGTQTLGKPMTKTEIDYIASAFALASRRAQESGFDG
VEIHAAHTYLINQFLSPYYNQREDEYGGSLENRMRFLLDIYTEIRKRVGESYPILVKLTASEFFDGGLTFDETRLICKKL
ESVGVDAIIISGNIHGNACTVVGESFDGHAIQQEGYFREYGAVISQEVNLPIITVGGLADFCAIEEIAATTNIQFFALSR
PLLAEPQLIKRWSDGRRAEVDCERCSKCRTKRGNFCVVYKKRNAA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=352, Percent_Identity=29.2613636363636, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787939, Length=215, Percent_Identity=33.0232558139535, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17540738, Length=353, Percent_Identity=30.028328611898, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17559802, Length=348, Percent_Identity=31.6091954022989, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17565138, Length=336, Percent_Identity=29.4642857142857, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI72001454, Length=349, Percent_Identity=30.3724928366762, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17559804, Length=352, Percent_Identity=29.8295454545455, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17564188, Length=349, Percent_Identity=29.2263610315186, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17566914, Length=288, Percent_Identity=29.5138888888889, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17566916, Length=351, Percent_Identity=26.2108262108262, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=223, Percent_Identity=27.8026905829596, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=224, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40887; Mature: 40756

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLFSPARIGKMTLKNRFVRSATWENMATEDGHMTDKLYSVYEELAKGEVGLIVTGYAN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHH
IVAEEKPNAGMMGMYNDSFIAEYQKLTELVHKYDSKIVMQLAYGGTKTTYNVGERVIFAP
EEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCEEEECC
SAVAERGTQTLGKPMTKTEIDYIASAFALASRRAQESGFDGVEIHAAHTYLINQFLSPYY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
NQREDEYGGSLENRMRFLLDIYTEIRKRVGESYPILVKLTASEFFDGGLTFDETRLICKK
CCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHH
LESVGVDAIIISGNIHGNACTVVGESFDGHAIQQEGYFREYGAVISQEVNLPIITVGGLA
HHHCCCCEEEEECCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCEEEECCCH
DFCAIEEIAATTNIQFFALSRPLLAEPQLIKRWSDGRRAEVDCERCSKCRTKRGNFCVVY
HHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEE
KKRNAA
ECCCCC
>Mature Secondary Structure 
STLFSPARIGKMTLKNRFVRSATWENMATEDGHMTDKLYSVYEELAKGEVGLIVTGYAN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHH
IVAEEKPNAGMMGMYNDSFIAEYQKLTELVHKYDSKIVMQLAYGGTKTTYNVGERVIFAP
EEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCEEEECC
SAVAERGTQTLGKPMTKTEIDYIASAFALASRRAQESGFDGVEIHAAHTYLINQFLSPYY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
NQREDEYGGSLENRMRFLLDIYTEIRKRVGESYPILVKLTASEFFDGGLTFDETRLICKK
CCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHH
LESVGVDAIIISGNIHGNACTVVGESFDGHAIQQEGYFREYGAVISQEVNLPIITVGGLA
HHHCCCCEEEEECCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCEEEECCCH
DFCAIEEIAATTNIQFFALSRPLLAEPQLIKRWSDGRRAEVDCERCSKCRTKRGNFCVVY
HHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEE
KKRNAA
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]