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The map label for this gene is mleN [H]

Identifier: 167622019

GI number: 167622019

Start: 88218

End: 89630

Strand: Reverse

Name: mleN [H]

Synonym: Shal_0078

Alternate gene names: 167622019

Gene position: 89630-88218 (Counterclockwise)

Preceding gene: 167622020

Following gene: 167622018

Centisome position: 1.71

GC content: 50.39

Gene sequence:

>1413_bases
ATGTCTCAGTCAACAAAGCTTGAGTTTCATATAGGAAAAATCGGTGGAATGGTTCCACTGTTGACCATGGTCGGTCTGAT
GTTACTGCTTACCTTTAATGGTGTTGGTGGCCCTAAAGCCGCATGGGCACCTGGCTTTATCGCCATTATTTTAGGTATGT
TCCTAGTTAAGAGTAAACGTGACTATTTTCAGTCGATTTTAGACGGTATTGCTGATAAAACTGGTGTTGTGGTGATCACA
GCCTGGATCTTCGCCGCCGTACTCGGCTCGTTAATGAAGGCCGGTGGCCTAGTCGACGGTATTCTTTGGTTTGGTATGTC
GACCGGTATTCAGGGCAGCATGTTCCTTATTATCGTGTTCATGTCTACTGCCATCTTCGCCATGGGTACTGGCTCGTCAA
ACGGTGCCAACTTAGCCCTTGCTCCTATCCTATTCCCAGCCGGTGTTTACCTAGGCGCAGATCCAGCTTTTGTTGCTCTT
GCTCTACTGTCTGGTGCCGTATTTGGCGATAACATTGCGCCAATTTCCGATACTACCATCGTAGGCTGTACCACACAGGG
AGCCGACTTTGGCGCCACGGTTCGCCAACGTATGCCGCTATCACTGACTGCCGGAGCTATAGCACTCGTCGCCTTGGTAT
TCTTTGGTCAAGGTGATGGCCTAGTCGAAGCGGCCACCCAAAGCGGTGATAGCGCCCGCCCAGAAAGCCTACTAATGTTG
CTCTCTGTTGCTGCCGTTGTGTTCTTCGCCCTAAAAGGTCGCCACCTGTTAGAAGCCCTAAGCTACGGTATCCTTGTTGC
AGCCGTTGTTGGACTCGGCATCGGTGCCTTCGCGATTTCGGATCTATTCACCGTTCCATCTCAGCGCGGCGTCTCTAACG
GTATCATCGAAGACGCTATTGCCGGTGTAACTGGTGCGGTCGTATTCGTAATCCTAATTTTGGGCGTTATTAAGATCTTC
ATGAATAGCGGCATCATGGATGACATTCTCAAGTTCATCACAGAACGCGTTTCAAATAGTACCCGTAGCACCGAGCTCTT
GATGTTCTACTCAACATCAGTGTCGTCACTATTGGTGAGCTCGAATGCACCGTCTCAGCTACTCGTTGGCCCGACTCTAG
TCAAGCCACTAGGTGAGAAGAACAACATTACCCCTGAGCGTCGCGCCAACCTAATGAGTGCCTCTGTGTGTTCAATCTTC
TACATGATGCCTTGGTGTCTTGCGGTGATGGTCTGGTACTCGGCAATCGAAACCTCAGCTATCTCTTCTGGCTTAGCGGT
TCCGAGTGCATCGATCAGCTTTATGGCACCTTACCCATGGGCCCTACTGGGTGTAATGATGTTCTCTATCATCACGGGTT
GGAAGCGCTACGGCAAACAGGCTCAACCCCAAGCTCAAGTACAGCCAGCCTAA

Upstream 100 bases:

>100_bases
CATGCCTGAGAGTTTTCTCAGGCATTTTGGCCAAACTTTATATTCCTCGTCCCAACAGGCTACCCCTGCAGCGATGACGA
AGGAATAGGTAGAATCTATT

Downstream 100 bases:

>100_bases
TTGCAACTAATACAATAAACGAATAACAATAACTGTGGCGGCGTAGTCCGTCACAGATAAAAACAGGAAATAGAAATGAC
TAAAGCACTTAACACTGAAT

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKRDYFQSILDGIADKTGVVVIT
AWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVFMSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVAL
ALLSGAVFGDNIAPISDTTIVGCTTQGADFGATVRQRMPLSLTAGAIALVALVFFGQGDGLVEAATQSGDSARPESLLML
LSVAAVVFFALKGRHLLEALSYGILVAAVVGLGIGAFAISDLFTVPSQRGVSNGIIEDAIAGVTGAVVFVILILGVIKIF
MNSGIMDDILKFITERVSNSTRSTELLMFYSTSVSSLLVSSNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIF
YMMPWCLAVMVWYSAIETSAISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGKQAQPQAQVQPA

Sequences:

>Translated_470_residues
MSQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKRDYFQSILDGIADKTGVVVIT
AWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVFMSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVAL
ALLSGAVFGDNIAPISDTTIVGCTTQGADFGATVRQRMPLSLTAGAIALVALVFFGQGDGLVEAATQSGDSARPESLLML
LSVAAVVFFALKGRHLLEALSYGILVAAVVGLGIGAFAISDLFTVPSQRGVSNGIIEDAIAGVTGAVVFVILILGVIKIF
MNSGIMDDILKFITERVSNSTRSTELLMFYSTSVSSLLVSSNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIF
YMMPWCLAVMVWYSAIETSAISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGKQAQPQAQVQPA
>Mature_469_residues
SQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKRDYFQSILDGIADKTGVVVITA
WIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVFMSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVALA
LLSGAVFGDNIAPISDTTIVGCTTQGADFGATVRQRMPLSLTAGAIALVALVFFGQGDGLVEAATQSGDSARPESLLMLL
SVAAVVFFALKGRHLLEALSYGILVAAVVGLGIGAFAISDLFTVPSQRGVSNGIIEDAIAGVTGAVVFVILILGVIKIFM
NSGIMDDILKFITERVSNSTRSTELLMFYSTSVSSLLVSSNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIFY
MMPWCLAVMVWYSAIETSAISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGKQAQPQAQVQPA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 49128; Mature: 48997

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKR
CCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DYFQSILDGIADKTGVVVITAWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
MSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVALALLSGAVFGDNIAPISDTTI
HHHHHHHCCCCCCCCCCEEEHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEE
VGCTTQGADFGATVRQRMPLSLTAGAIALVALVFFGQGDGLVEAATQSGDSARPESLLML
EEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHH
LSVAAVVFFALKGRHLLEALSYGILVAAVVGLGIGAFAISDLFTVPSQRGVSNGIIEDAI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AGVTGAVVFVILILGVIKIFMNSGIMDDILKFITERVSNSTRSTELLMFYSTSVSSLLVS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHC
SNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIFYMMPWCLAVMVWYSAIETSA
CCCCCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGKQAQPQAQVQPA
HHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKR
CCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DYFQSILDGIADKTGVVVITAWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
MSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVALALLSGAVFGDNIAPISDTTI
HHHHHHHCCCCCCCCCCEEEHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEE
VGCTTQGADFGATVRQRMPLSLTAGAIALVALVFFGQGDGLVEAATQSGDSARPESLLML
EEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHH
LSVAAVVFFALKGRHLLEALSYGILVAAVVGLGIGAFAISDLFTVPSQRGVSNGIIEDAI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AGVTGAVVFVILILGVIKIFMNSGIMDDILKFITERVSNSTRSTELLMFYSTSVSSLLVS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHC
SNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIFYMMPWCLAVMVWYSAIETSA
CCCCCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGKQAQPQAQVQPA
HHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]