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The map label for this gene is pdhB [H]

Identifier: 162147505

GI number: 162147505

Start: 1744280

End: 1745308

Strand: Reverse

Name: pdhB [H]

Synonym: GDI_1721

Alternate gene names: 162147505

Gene position: 1745308-1744280 (Counterclockwise)

Preceding gene: 162147506

Following gene: 162147504

Centisome position: 44.25

GC content: 67.06

Gene sequence:

>1029_bases
ATGAGCAAGAAGAGCTATCGGCAGGCCATCAACGAGGCCCTGCGACTGGAAATGCGGCGCGACCCGCGCGTGATCCTGAT
GGGCGAGGACGTCGCCGGCGGACATGGCGGATCGTCGGGCGTCACCGACGCCTGGGGCGGCGTGCTGGGCGTCACCAAGG
GCCTGTTGAGCGAATTCGGCGAGGATCGCGTCCTGGACACCCCGATCACGGAAGCATCCTATATCGGCGCCGCCGCCGGG
GCCGCCGCGACCGGCCTACGCCCCGTCGCCGAGCTGATGTTCGTCGATTTCGTGGGCTGCTGCCTGGACCAGATCATGAA
CCAGGCCGCCAAGTTCCGCTACATGTTCGGCGGCAAGGCCCGCACCCCGCTGGTCATCCGCGCCATGTTCGGCGCCGGCT
TCAACGCCGCGGCCCAGCACAGCCAGGCGCTGTACCCGCTGTTCACCCACATTCCCGGGCTGAAGGTGGTCGTCCCGTCC
TCGCCCTACGAGGCCAAGGGCCTGCTGATCGAGGCGATCCGCGACGACGATCCGGTGATCTTCCTTGAACACAAGGTCAT
GTATGACGACGAGGAAGAGGTGCCCGACGAAGCCTATACCATCCCGTTCGGCGAGGCCAACCTGACGCGTGAGGGCGACG
ACCTGACGATCGTGGCGTTCGGCCGCATGGTGAAGCTGGCGAACGAGGCCGCCGACCGGCTGCAAAAGCAGGGCATCGGC
TGCACCGTCATCGATCCGCGCACCACCTCGCCGCTGGATGCCGAGACGATCCTGGACAGCGTGACCGAGACCGGCCGGCT
GGTGATCGTCGATGAATCCAGCCCGCGCTGCAACATGGCCGCCGACATCTCCGCCCTGGTGGCCGAACAGGCGTTCGACG
CGCTGAAGGCCCCGATCCGGCGGGTGATGCCACCCCACACGCCGGTGCCGTTCGCATCGGTGCTGGAAAGCCTGTACCTG
CCCGACGTGGCGAAGATCGAAGCGGCTGCCCGTGCCGTGATGACCCATCGCATCCGAGAGGTCGCCTGA

Upstream 100 bases:

>100_bases
GGCCCCGCTGCCCGAACCCGAGGACCTGCTGGCCGATGTCTATGTCCGTTACTGATCCCGCCCGCATCTCCCGGTTCCTG
TCAGCCTGAAAGACATAAAA

Downstream 100 bases:

>100_bases
ACCATGACTGATTCCATTACGCCCATCACGATGCCCAAGTTCGGCCTGGCGATGACCGAGGGCAAGCTGGCCGGCTGGAT
GGTCCGCCCCGGTACCAGCG

Product: pyruvate dehydrogenase E1 component subunit beta

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSKKSYRQAINEALRLEMRRDPRVILMGEDVAGGHGGSSGVTDAWGGVLGVTKGLLSEFGEDRVLDTPITEASYIGAAAG
AAATGLRPVAELMFVDFVGCCLDQIMNQAAKFRYMFGGKARTPLVIRAMFGAGFNAAAQHSQALYPLFTHIPGLKVVVPS
SPYEAKGLLIEAIRDDDPVIFLEHKVMYDDEEEVPDEAYTIPFGEANLTREGDDLTIVAFGRMVKLANEAADRLQKQGIG
CTVIDPRTTSPLDAETILDSVTETGRLVIVDESSPRCNMAADISALVAEQAFDALKAPIRRVMPPHTPVPFASVLESLYL
PDVAKIEAAARAVMTHRIREVA

Sequences:

>Translated_342_residues
MSKKSYRQAINEALRLEMRRDPRVILMGEDVAGGHGGSSGVTDAWGGVLGVTKGLLSEFGEDRVLDTPITEASYIGAAAG
AAATGLRPVAELMFVDFVGCCLDQIMNQAAKFRYMFGGKARTPLVIRAMFGAGFNAAAQHSQALYPLFTHIPGLKVVVPS
SPYEAKGLLIEAIRDDDPVIFLEHKVMYDDEEEVPDEAYTIPFGEANLTREGDDLTIVAFGRMVKLANEAADRLQKQGIG
CTVIDPRTTSPLDAETILDSVTETGRLVIVDESSPRCNMAADISALVAEQAFDALKAPIRRVMPPHTPVPFASVLESLYL
PDVAKIEAAARAVMTHRIREVA
>Mature_341_residues
SKKSYRQAINEALRLEMRRDPRVILMGEDVAGGHGGSSGVTDAWGGVLGVTKGLLSEFGEDRVLDTPITEASYIGAAAGA
AATGLRPVAELMFVDFVGCCLDQIMNQAAKFRYMFGGKARTPLVIRAMFGAGFNAAAQHSQALYPLFTHIPGLKVVVPSS
PYEAKGLLIEAIRDDDPVIFLEHKVMYDDEEEVPDEAYTIPFGEANLTREGDDLTIVAFGRMVKLANEAADRLQKQGIGC
TVIDPRTTSPLDAETILDSVTETGRLVIVDESSPRCNMAADISALVAEQAFDALKAPIRRVMPPHTPVPFASVLESLYLP
DVAKIEAAARAVMTHRIREVA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=336, Percent_Identity=40.4761904761905, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI4557353, Length=338, Percent_Identity=39.9408284023669, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI34101272, Length=338, Percent_Identity=39.9408284023669, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI291084858, Length=336, Percent_Identity=38.6904761904762, Blast_Score=223, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17538422, Length=335, Percent_Identity=41.1940298507463, Blast_Score=247, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI17506935, Length=339, Percent_Identity=39.5280235988201, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319698, Length=335, Percent_Identity=42.089552238806, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI21358145, Length=343, Percent_Identity=39.6501457725948, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24650940, Length=343, Percent_Identity=39.6501457725948, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI160714828, Length=331, Percent_Identity=36.2537764350453, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI160714832, Length=331, Percent_Identity=36.2537764350453, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24650943, Length=100, Percent_Identity=41, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650945, Length=100, Percent_Identity=41, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36906; Mature: 36775

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKSYRQAINEALRLEMRRDPRVILMGEDVAGGHGGSSGVTDAWGGVLGVTKGLLSEFG
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EDRVLDTPITEASYIGAAAGAAATGLRPVAELMFVDFVGCCLDQIMNQAAKFRYMFGGKA
CCCEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCC
RTPLVIRAMFGAGFNAAAQHSQALYPLFTHIPGLKVVVPSSPYEAKGLLIEAIRDDDPVI
CCCEEEEEHHCCCCCHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCEE
FLEHKVMYDDEEEVPDEAYTIPFGEANLTREGDDLTIVAFGRMVKLANEAADRLQKQGIG
EEEEEEECCCHHHCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
CTVIDPRTTSPLDAETILDSVTETGRLVIVDESSPRCNMAADISALVAEQAFDALKAPIR
EEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RVMPPHTPVPFASVLESLYLPDVAKIEAAARAVMTHRIREVA
HHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKSYRQAINEALRLEMRRDPRVILMGEDVAGGHGGSSGVTDAWGGVLGVTKGLLSEFG
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EDRVLDTPITEASYIGAAAGAAATGLRPVAELMFVDFVGCCLDQIMNQAAKFRYMFGGKA
CCCEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCC
RTPLVIRAMFGAGFNAAAQHSQALYPLFTHIPGLKVVVPSSPYEAKGLLIEAIRDDDPVI
CCCEEEEEHHCCCCCHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCEE
FLEHKVMYDDEEEVPDEAYTIPFGEANLTREGDDLTIVAFGRMVKLANEAADRLQKQGIG
EEEEEEECCCHHHCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
CTVIDPRTTSPLDAETILDSVTETGRLVIVDESSPRCNMAADISALVAEQAFDALKAPIR
EEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RVMPPHTPVPFASVLESLYLPDVAKIEAAARAVMTHRIREVA
HHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]