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The map label for this gene is pdhA [H]

Identifier: 162147506

GI number: 162147506

Start: 1745354

End: 1746319

Strand: Reverse

Name: pdhA [H]

Synonym: GDI_1722

Alternate gene names: 162147506

Gene position: 1746319-1745354 (Counterclockwise)

Preceding gene: 162147508

Following gene: 162147505

Centisome position: 44.28

GC content: 67.81

Gene sequence:

>966_bases
ATGCAGATCGCGCGGGAAACGTTGCTTCGCTCCTATCGCGCCATGCGGACGATCCGTGATTTCGAGGAACGGCTGCATGT
CGAATTCGCGACGGGCGAAATCCCCGGCTTCGTCCACCTCTATTGCGGCGAGGAAGCGTCAGGCGTCGGCGTCTGTGCCA
ACCTGACCGATACCGACACCATCTCCAGCACCCATCGTGGCCATGGCCACTGCATCGCCAAGGGGGTCGAGGTCGCGGGC
ATGATGGCCGAGATCTACGGCCGTCGCACCGGCGTGTGCCGGGGCAAGGGCGGGTCGATGCACATCGCCGACCTGTCGCG
CGGCATGCTGGGCGCGAACGGCATCGTGGGCGGCGGCCCGCCGCTGATGTGCGGCGCCGCCCTGGCACACAAGACGCTGA
AGGATGGCGGCGTCGCGGTCGCGTTCTACGGCGACGGCGCGTCGAACGAGGGCTCGACGCTGGAAAGCCTCAACCTCGCG
TCGGTCTGGCGCCTGCCGGTGGTCTTCGTGCTGGAGGATAACGGCTACGGCGAGGCCACGGCGTCGTCCTACGCCTGCGC
CGGATCGCAGAAGGCCCGGGCCGAAGGGTTCGGCATGCCCTACATGGAATGTGACGGGTCGGATTTCTTCGCGGTGCACC
AGGCGGCGGCCGAAGCCATCGCCCATGCCCGCGCCGGAAAGGGCCCCGTCATGCTGCATGTCCATCTGGCGCGCTGGTAC
GGGCATTTCGAAGGCGATGCCATGACCTATCGCGCGTCCGGCGAAGTCGCGGCGGAACGGCGCGACCGTGACTGCCTGGC
GCTGTTCCGCACCCGCGTGACCGAGGCCGGCCTGCTGGAGCATTCCGACCTGGACGACATCGACGCCTCGGTCCGTGACG
AGATCGAAAGCGCGGTCGTCGCCGCGAAGCAGGCCCCGCTGCCCGAACCCGAGGACCTGCTGGCCGATGTCTATGTCCGT
TACTGA

Upstream 100 bases:

>100_bases
ATTCGTATAAGAGAATTTCTTGCTGAAAAGGCGGATCGGCGTTCCCGTGGGATCACGGATTTCGGTTCCGATATCCCATG
AATAACAAGGAAGATTTCCA

Downstream 100 bases:

>100_bases
TCCCGCCCGCATCTCCCGGTTCCTGTCAGCCTGAAAGACATAAAAATGAGCAAGAAGAGCTATCGGCAGGCCATCAACGA
GGCCCTGCGACTGGAAATGC

Product: pyruvate dehydrogenase E1 component subunit alpha

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQIARETLLRSYRAMRTIRDFEERLHVEFATGEIPGFVHLYCGEEASGVGVCANLTDTDTISSTHRGHGHCIAKGVEVAG
MMAEIYGRRTGVCRGKGGSMHIADLSRGMLGANGIVGGGPPLMCGAALAHKTLKDGGVAVAFYGDGASNEGSTLESLNLA
SVWRLPVVFVLEDNGYGEATASSYACAGSQKARAEGFGMPYMECDGSDFFAVHQAAAEAIAHARAGKGPVMLHVHLARWY
GHFEGDAMTYRASGEVAAERRDRDCLALFRTRVTEAGLLEHSDLDDIDASVRDEIESAVVAAKQAPLPEPEDLLADVYVR
Y

Sequences:

>Translated_321_residues
MQIARETLLRSYRAMRTIRDFEERLHVEFATGEIPGFVHLYCGEEASGVGVCANLTDTDTISSTHRGHGHCIAKGVEVAG
MMAEIYGRRTGVCRGKGGSMHIADLSRGMLGANGIVGGGPPLMCGAALAHKTLKDGGVAVAFYGDGASNEGSTLESLNLA
SVWRLPVVFVLEDNGYGEATASSYACAGSQKARAEGFGMPYMECDGSDFFAVHQAAAEAIAHARAGKGPVMLHVHLARWY
GHFEGDAMTYRASGEVAAERRDRDCLALFRTRVTEAGLLEHSDLDDIDASVRDEIESAVVAAKQAPLPEPEDLLADVYVR
Y
>Mature_321_residues
MQIARETLLRSYRAMRTIRDFEERLHVEFATGEIPGFVHLYCGEEASGVGVCANLTDTDTISSTHRGHGHCIAKGVEVAG
MMAEIYGRRTGVCRGKGGSMHIADLSRGMLGANGIVGGGPPLMCGAALAHKTLKDGGVAVAFYGDGASNEGSTLESLNLA
SVWRLPVVFVLEDNGYGEATASSYACAGSQKARAEGFGMPYMECDGSDFFAVHQAAAEAIAHARAGKGPVMLHVHLARWY
GHFEGDAMTYRASGEVAAERRDRDCLALFRTRVTEAGLLEHSDLDDIDASVRDEIESAVVAAKQAPLPEPEDLLADVYVR
Y

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=317, Percent_Identity=35.6466876971609, Blast_Score=204, Evalue=8e-53,
Organism=Homo sapiens, GI4505685, Length=317, Percent_Identity=36.2776025236593, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI291084742, Length=317, Percent_Identity=36.2776025236593, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI291084744, Length=324, Percent_Identity=35.4938271604938, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI291084757, Length=317, Percent_Identity=31.8611987381703, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI258645172, Length=318, Percent_Identity=26.4150943396226, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI11386135, Length=319, Percent_Identity=26.3322884012539, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17536047, Length=317, Percent_Identity=37.8548895899054, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32564172, Length=317, Percent_Identity=37.8548895899054, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI86563355, Length=316, Percent_Identity=27.2151898734177, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI86563357, Length=316, Percent_Identity=27.2151898734177, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=36.9565217391304, Blast_Score=205, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24639748, Length=321, Percent_Identity=36.7601246105919, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24639740, Length=319, Percent_Identity=36.9905956112853, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24639744, Length=319, Percent_Identity=36.9905956112853, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28571106, Length=319, Percent_Identity=36.9905956112853, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24639746, Length=305, Percent_Identity=38.0327868852459, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21355903, Length=322, Percent_Identity=22.9813664596273, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 34301; Mature: 34301

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIARETLLRSYRAMRTIRDFEERLHVEFATGEIPGFVHLYCGEEASGVGVCANLTDTDT
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCC
ISSTHRGHGHCIAKGVEVAGMMAEIYGRRTGVCRGKGGSMHIADLSRGMLGANGIVGGGP
CCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHCCCCCCCCCCCCCC
PLMCGAALAHKTLKDGGVAVAFYGDGASNEGSTLESLNLASVWRLPVVFVLEDNGYGEAT
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCHHHEECCCEEEEEECCCCCCCC
ASSYACAGSQKARAEGFGMPYMECDGSDFFAVHQAAAEAIAHARAGKGPVMLHVHLARWY
CCCEECCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHC
GHFEGDAMTYRASGEVAAERRDRDCLALFRTRVTEAGLLEHSDLDDIDASVRDEIESAVV
CCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AAKQAPLPEPEDLLADVYVRY
HHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQIARETLLRSYRAMRTIRDFEERLHVEFATGEIPGFVHLYCGEEASGVGVCANLTDTDT
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCC
ISSTHRGHGHCIAKGVEVAGMMAEIYGRRTGVCRGKGGSMHIADLSRGMLGANGIVGGGP
CCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHCCCCCCCCCCCCCC
PLMCGAALAHKTLKDGGVAVAFYGDGASNEGSTLESLNLASVWRLPVVFVLEDNGYGEAT
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCHHHEECCCEEEEEECCCCCCCC
ASSYACAGSQKARAEGFGMPYMECDGSDFFAVHQAAAEAIAHARAGKGPVMLHVHLARWY
CCCEECCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHC
GHFEGDAMTYRASGEVAAERRDRDCLALFRTRVTEAGLLEHSDLDDIDASVRDEIESAVV
CCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AAKQAPLPEPEDLLADVYVRY
HHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]