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The map label for this gene is dmlR [H]

Identifier: 161870774

GI number: 161870774

Start: 1899369

End: 1900187

Strand: Direct

Name: dmlR [H]

Synonym: NMCC_1856

Alternate gene names: 161870774

Gene position: 1899369-1900187 (Clockwise)

Preceding gene: 161870769

Following gene: 161870775

Centisome position: 88.2

GC content: 59.22

Gene sequence:

>819_bases
ATGGCAAGCAAACACGTCAGCCACTTGGAAAACACCGTCCAAGCCAAACTCCTGCACCGCAACAGCCGCAACCTCAGCCT
GACCGAAGCCGGGGAAGAATACTACCGGCAATGCAGTTACGCGCTCGACACGCTCGACGATGCCGCGCAAAAAGCCGCCG
GGGGGACGGAAAAACCGCAGGGGCTGCTGCGCGTAACGATGCCACTGTGGTTTGCCGGCAGCCAGATATGCAACTGGCTG
GCGGAATACCGCGAACGTTATCCCGAAGTGACATTAGAACTGATTTTGGACAACCGCCACGTCGATTTGATTGCCGAAGG
CGTGGATTTGGCGTTGCGCGTTTCCCAAACCCTGTCCCCGTCGCTGATTGCGCGCCCGCTGGCGGAAATCGAATTTGCCC
TGCTCGCCTCGCCCGATTTCCTGAGGCGCAACGGCGTACCGGAAACGCCGGAAGAAGTGGCGGGGCTGCCCGCCGTCCTG
CCGACCTACACCAACCAGCAGAAACTCGACCTCACCCGCAAATCGGACGGCAAAAAATACCGGCTTGAACTGACCCCCGT
CATCCGTACCGACAACACGCTGATGATGCGCGAAATGATTAAGGCGGGCGCGTGCATCGGTTATCAGCCGCTTTGGGCGG
CGGAACACGATTTGCGCTGCGGCTCGCTGGTGAGGCTGCTGCCCGAATATACCGTCCCGACCGACCGCCTGAATGCCGTT
TATGCGGACAGGGCGTTCTTAAGCGCGAAAGTCCGCAGCTTCATCGATTTTCTGAACGAAAAAATCGCCAGCAGGAAAGG
CTGCCGAAATGCCGTCTGA

Upstream 100 bases:

>100_bases
AGAAAATATGGACACCCTGTTCAGCCTCAAGGTTTTCCGCCAAGTCGTCCAAAGCGGCGGCTTCACCCGCGCCGCCGACG
CGCTCGGCATCTCCACCGCA

Downstream 100 bases:

>100_bases
AACCGCCCGCCCCCTTTATGCGGACACGTGCGCCGCACACACGCTCGTCTGGTTCCGCCAAAACCTCCGCATCCGCGACA
ACGCCGCCTTATGCGCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MASKHVSHLENTVQAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWFAGSQICNWL
AEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAEIEFALLASPDFLRRNGVPETPEEVAGLPAVL
PTYTNQQKLDLTRKSDGKKYRLELTPVIRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAV
YADRAFLSAKVRSFIDFLNEKIASRKGCRNAV

Sequences:

>Translated_272_residues
MASKHVSHLENTVQAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWFAGSQICNWL
AEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAEIEFALLASPDFLRRNGVPETPEEVAGLPAVL
PTYTNQQKLDLTRKSDGKKYRLELTPVIRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAV
YADRAFLSAKVRSFIDFLNEKIASRKGCRNAV
>Mature_271_residues
ASKHVSHLENTVQAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWFAGSQICNWLA
EYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAEIEFALLASPDFLRRNGVPETPEEVAGLPAVLP
TYTNQQKLDLTRKSDGKKYRLELTPVIRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVY
ADRAFLSAKVRSFIDFLNEKIASRKGCRNAV

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=28.8973384030418, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786401, Length=269, Percent_Identity=30.8550185873606, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1789639, Length=258, Percent_Identity=28.2945736434109, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789440, Length=267, Percent_Identity=23.5955056179775, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693193, Length=258, Percent_Identity=27.1317829457364, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787128, Length=264, Percent_Identity=23.1060606060606, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787589, Length=265, Percent_Identity=24.1509433962264, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30558; Mature: 30427

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKHVSHLENTVQAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQ
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLLRVTMPLWFAGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSP
CCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHCCHHHEEHHHHHCCH
SLIARPLAEIEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKY
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHEECCCCCCCCEE
RLELTPVIRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAV
EEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
YADRAFLSAKVRSFIDFLNEKIASRKGCRNAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASKHVSHLENTVQAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQ
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLLRVTMPLWFAGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSP
CCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHCCHHHEEHHHHHCCH
SLIARPLAEIEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKY
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHEECCCCCCCCEE
RLELTPVIRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAV
EEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
YADRAFLSAKVRSFIDFLNEKIASRKGCRNAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]