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The map label for this gene is pilG [H]

Identifier: 161870737

GI number: 161870737

Start: 1856654

End: 1857886

Strand: Direct

Name: pilG [H]

Synonym: NMCC_1810

Alternate gene names: 161870737

Gene position: 1856654-1857886 (Clockwise)

Preceding gene: 161870733

Following gene: 161870738

Centisome position: 86.22

GC content: 52.72

Gene sequence:

>1233_bases
ATGGCTAAAAACGGAGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCCGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCCGCGTTTACCGAAGAAGAGGCGCGCAAAAAACTGGCAAAACGCGGCATCCGCCCGT
TGCAGATTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATCACCGTTTTCACCCGCCAGCTGTCC
ACGATGATTAAAGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGAGGCGAAGTGGAACAGGGCAGCTCGTTGAGCCGCGCATTCTCAAACCACCCAAAATATTTCG
ACCGCTTCTACTGCAATCTGGTTGCGGCGGGCGAAACGGGCGGCGTATTGGAAAGCCTGCTGGACAAATTGGCAATTTAC
AAAGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCACTGACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAAGTTTACGCCAATATGGGCGCGGAGCTTCCCGCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTGGGCTTTGCCATA
TACGGCTTCCTTAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCATACTGCTGCGTATGCCGATTTT
CGGAGACATTGTCCGCAAAGGGACGATTGCCCGCTGGGGCAGGACGACGGCGACGCTGATTGCGGCAGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGTTTGTCTATGACTTCGGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGTTGCAGATGTCTTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTACGAAGACGAGGTGGACAATGCGGTCGGCA
GGCTGTCCGCTATGATGGAGCCGATCATTATCGTGATTTTAGGCTTGGTCATCGGCACGCTTCTGGTCGCCATGTATCTG
CCGCTGTTTAATTTGGGCAACGTGGTCGCCTGA

Upstream 100 bases:

>100_bases
GGCGGGATGTCGGCGGTTTTCATCTTACCGGGAACACGCCGGTACTGTTTTTTAACGATGTTTCTTACATTTTATTCCAA
TTACTTTACGGGGCTAGAAT

Downstream 100 bases:

>100_bases
TTTGCCGCACAGATCCGGCGCGGATTCGCTCTGCGCCGGTTTGTTTTTGTTTTGAATACATTGAGAACAAAATATGTCTG
ATTTGTCTGTATTGTCGCCG

Product: type IV pilus assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVA

Sequences:

>Translated_410_residues
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVA
>Mature_409_residues
AKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLST
MIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIYK
EKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAIY
GFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVI
QGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYLP
LFNLGNVVA

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=385, Percent_Identity=31.6883116883117, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786295, Length=367, Percent_Identity=30.5177111716621, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45274; Mature: 45143

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVK
CCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]